Human placental cell culturing on collagen and fibronectin coated surfaces and fibroblast feeder-layer

Abstract

In this study, systems were established and tested for growing human placenta! trophoblasts on various surfaces. For this purpose, in the first step, trophoblastic cell were isolated from the first trimester human placentas. Trophoblastic cells were cultured on collagen and fibronectin coated surfaces and 3T3 fibroblast feeder-layer. To prepare collagen coated surfaces, six-well polystirene (PS) dishes weri modified by using collagen-glutaraldehyde (GTA) mixture. Fibronectin coated surfaces were prepared with the same method. Fibroblast feeder-layer was prepared by using 3T3 mouse fibroblasts. Syncytia formation capacity of trophoblastic cells were investigated on these surfaces and the results were compared with the conventional techniques. The results showed that trophoblasts adhered neither to the normal nor to the modified surfaces. But adhering and growing were observed on the fibroblast feeder-layer.