Logarithm of retention factor corresponding to pure water, log $k _w$, which is the most commonly employed chromatographic hydrophobicity parameter determined by using chromatographic data.
📌 3 Key Takeaways
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Objective & Hypothesis:In this work, the proportion of organic modifier of the hydro-organic mobile phase was optimized in order to separate twelve compounds namely sulfaguanidine, sulfanilamide, sulfadiazine, sulfathiazole, sulfamethazine, sulfamerazine, sulfamethoxypyridazine, sulfamonomethoxyne, sulfachloropyridazine, sulfadoxine, sulfafurazole and dapson using Symmetry Shield RP-8 Column.
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Key Findings & Data:Mobile phase was optimized by establishing relationships between retention parameters and Reichardt’s $R^T _N$ scale of solvent polarity.
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Conclusion & Impact:Logarithm of retention factor corresponding to pure water, log $k _w$, which is the most commonly employed chromatographic hydrophobicity parameter determined by using chromatographic data.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters
Abstract
In this work, the proportion of organic modifier of the hydro-organic mobile phase was optimized in order to separate twelve compounds namely sulfaguanidine, sulfanilamide, sulfadiazine, sulfathiazole, sulfamethazine, sulfamerazine, sulfamethoxypyridazine, sulfamonomethoxyne, sulfachloropyridazine, sulfadoxine, sulfafurazole and dapson using Symmetry Shield RP-8 Column. Mobile phase was optimized by establishing relationships between retention parameters and Reichardt’s $R^T _N$ scale of solvent polarity. In addition, selectivity (α) and resolution ($R _S$) values were used in order to establish a general model relating elution behavior of substances with the composition of mobile phase. Logarithm of retention factor corresponding to pure water, log $k _w$, which is the most commonly employed chromatographic hydrophobicity parameter determined by using chromatographic data.
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Cite This Article
ŞANLI, N., ŞANLI, S., GÖKMEŞE, F., DEMİRALAY, E. Ç., GÖKMEŞE, E. (2022). Optimization of separation of several sulfonamides in acetonitrile-water binary mixtures by using symmetry shield RP-8 column. Hacettepe Journal of Biology and Chemistry, Cilt: 37 - Sayı: 2, 103–110. https://ajindex.com/articles/529306
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