Journals / Hacettepe Journal of Biology and Chemistry / 2009 / Cilt: 37 - Sayı: 4

Affinity to some plant growth regulators on human erythrocytes Cytosolic Carbonic anhydrase I and II

Pages
281–288
Publication Date
2022-10-17
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conversely, the enzyme activity was increased by gibberellic acid and kinetin.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The purpose of this study was to investigate the in vitro effects of four commonly used plant growth regulators (PGRs): indole-3-acetic acid, indole-3-butyric acid, gibberellic acid and kinetin on human carbonic anhydrase I and II.
📊
Key Findings & Data:Carbonic anhydrase I and II from human erythrocytes was purified by Sepharose-4B-L-tyrosine-1-sulfonamide affinity gel. $IC _{50}$ values of the chemicals that caused inhibition were determined by means of activity percentage diagrams.
💡
Conclusion & Impact:Conversely, the enzyme activity was increased by gibberellic acid and kinetin.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The purpose of this study was to investigate the in vitro effects of four commonly used plant growth regulators (PGRs): indole-3-acetic acid, indole-3-butyric acid, gibberellic acid and kinetin on human carbonic anhydrase I and II. Carbonic anhydrase I and II from human erythrocytes was purified by Sepharose-4B-L-tyrosine-1-sulfonamide affinity gel. $IC _{50}$ values of the chemicals that caused inhibition were determined by means of activity percentage diagrams. The plant growth regulators used in this study effected the CA activity from human to various degrees. The concentrations of indole-3-acetic acid, indole-3-butyric acid and kinetin that inhibited 50% of the hCA I were 78.13 μM, 54.48 μM and 62.89 μM respectively. The concentrations of indole-3-acetic acid and indole-3-butyric acid that inhibited 50% of the hCA II were 75.47 μM and 38.05 μM, respectively. Conversely, the enzyme activity was increased by gibberellic acid and kinetin.

🔖

Cite This Article

ARSLAN, O., BAYAZTAŞ, S. (2022). Affinity to some plant growth regulators on human erythrocytes Cytosolic Carbonic anhydrase I and II. Hacettepe Journal of Biology and Chemistry, Cilt: 37 - Sayı: 4, 281–288. https://ajindex.com/articles/529304

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (2)

  1. Supuran, C. T., Scozzafava, A., Conway, J. Carbonic Anhydrase-Its Inhibitors and Activators; CRC Press:
  2. Boca Raton, New York, London, p 1., 2004 2. Thiry, A., Dogne, J.M., Masereel, B., Supuran, C.T. Targeting tumor-associated carbonic anhydrase IX in cancer therapy. Trends Pharmacol. Sci., 27, 566, 2006. 3. McKierman, J.M., Buttyan, R., Stifelman, M.D., Kutz, A.G., Chen, M.W., Olsson, C.A., Sawczuk, I.S. Expression of tumor-associated gene MN: a potential biomarker of human renal cell carcinoma. Cancer Res. 57, 2362, 1997. 4. Scozzafava, A., Mastrolorenzo, A., Supuran, C.T. Carbonic anhydrase inhibitors and activators and their use in therapy. Expert Opin. Ther. Pat., 16, 1627, 2006. 5. Supuran, C.T., Scozzafava, A., Casini, A. Carbonic anhydrase inhibitors. Med. Res. Rev. 23, 146, 2003. 6. Smith, K.S., Ferry, J. G. Prokaryotic carbonic anhydrases. FEMS Microbiol. Rev. 24, 335, 2000. 7. Thiry, A. et al. Targeting tumor-associated carbonic anhydrase IX in cancer therapy. Trends Pharmacol. Sci. 27, 566, 2006. 8. Stams, T., Christianson, D.W. in The Carbonic Anhydrases — New Horizons (eds Chegwidden, W. R., Carter, N. D. & Edwards, Y. H.), 159, 2000. 9. Pastorekova, S. et al. Carbonic anhydrases: current state of the art, therapeutic applications and future prospects. J. Enzyme Inhib. Med. Chem., 19, 199, 2004. 10. Nishimori, I. et al. Carbonic anhydrase inhibitors. Cloning, characterization and inhibition studies of the cytosolic isozyme III with sulfonamides. Bioorg. Med. Chem. 15, 7229, 2007. 11. Vullo, D. et al. Carbonic anhydrase inhibitors. Inhibition of mitochondrial isozyme V with aromatic and heterocyclic sulfonamides. J. Med. Chem., 47, 1272, 2004. 12. Nishimori, I. et al. Carbonic anhydrase inhibitors. The mitochondrial isozyme VB as a new target for sulfonamide and sulfamate inhibitors. J. Med. Chem. 48, 7860, 2005. 13. Nishimori, I. et al. Carbonic anhydrase inhibitors. DNA cloning, characterization and inhibition studies of the human secretory isoform VI, a new target for sulfonamide and sulfamate inhibitors. J. Med. Chem. 50, 381, 2007. 14. Vullo, D. et al. Carbonic anhydrase inhibitors. Inhibition of the human cytosolic isozyme VII with aromatic and heterocyclic sulfonamides. Bioorg. Med. Chem. Lett., 15, 971, 2005. 15. Nishimori, I. in Carbonic Anhydrase — Its Inhibitors and Activators (eds Supuran, C. T., Scozzafava, A. & Conway J.), 25, 2004. 16. Vullo, D., et al. Carbonic anhydrase inhibitors. Inhibition of the tumor-associated isozyme