SavedAdd your journal

Journals / Hacettepe Journal of Biology and Chemistry / 2011 / Cilt: 39 - Sayı: 2

Bazı organik bileşikler ve amino asitlerin glukoz 6-fosfat dehidrogenaz aktivatör özellikleri

Glucose 6-phosphate dehydrogenase activatory properties of some organic compounds and amino acids

Zafer Ömer ÖZDEMİR
Murat ŞENTÜRK
Deniz EKİNCİ
Pages
157–162
Publication Date
2022-10-17
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

For this purpose, initially erythrocyte glucose 6-phosphate dehydrogenase was managed to be purified 812-fold in a yield of 40.6% by using 2’,5’-ADP Sepharose 4B affinity gel. 4’-(Methylsulfonyl)acetophenone, 4-(methylsulfonyl)phenylacetic acid, tyrosine and phenylalanine exhibited activatory effects on the enzyme. % Activity values for these compounds were determined by plotting activity percentage versus [Activator].

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The aim of this study was to assess activatory potentials of 4’-(Methylsulfonyl)- acetophenone, 4-(methylsulfonyl) phenylacetic acid, tyrosine and phenylalanine on human erythrocyte glucose 6-phosphate dehydrogenase.
📊
Key Findings & Data:The aim of this study was to assess activatory potentials of 4’-(Methylsulfonyl)- acetophenone, 4-(methylsulfonyl) phenylacetic acid, tyrosine and phenylalanine on human erythrocyte glucose 6-phosphate dehydrogenase.
💡
Conclusion & Impact:For this purpose, initially erythrocyte glucose 6-phosphate dehydrogenase was managed to be purified 812-fold in a yield of 40.6% by using 2’,5’-ADP Sepharose 4B affinity gel. 4’-(Methylsulfonyl)acetophenone, 4-(methylsulfonyl)phenylacetic acid, tyrosine and phenylalanine exhibited activatory effects on the enzyme. % Activity values for these compounds were determined by plotting activity percentage versus [Activator].
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to assess activatory potentials of 4’-(Methylsulfonyl)- acetophenone, 4-(methylsulfonyl) phenylacetic acid, tyrosine and phenylalanine on human erythrocyte glucose 6-phosphate dehydrogenase. For this purpose, initially erythrocyte glucose 6-phosphate dehydrogenase was managed to be purified 812-fold in a yield of 40.6% by using 2’,5’-ADP Sepharose 4B affinity gel. 4’-(Methylsulfonyl)acetophenone, 4-(methylsulfonyl)phenylacetic acid, tyrosine and phenylalanine exhibited activatory effects on the enzyme. % Activity values for these compounds were determined by plotting activity percentage versus [Activator].

Özet

Bu çalışmanın amacı 4-(metilsülfonil)-asetofenon, 4-(metilsülfonil) fenilasetik asit, tirozin ve fenilalaninin insan eritrosit glukoz 6-fosfat dehidrogenaz üzerindeki aktivatör potansiyelini değerlendirmektir. Bu amaçla, öncelikle eritrosit glukoz 6-fosfat dehidrogenaz 2’,5’-ADP Sepharose 4B afinite jeli kullanılarak 812-kat, %40.6 verimle elde edildi. 4-(Metilsülfonil) fenilasetik asit, tirozin ve fenilalaninin enzim üzerinde aktivatör etkileri gösterdi. Bu bileşikler için % aktivite-[Aktivatör] grafiği kullanılarak % aktivite değerleri belirlendi.

🔖

Cite This Article

ÖZDEMİR, Z. Ö., ŞENTÜRK, M., EKİNCİ, D. (2022). Bazı organik bileşikler ve amino asitlerin glukoz 6-fosfat dehidrogenaz aktivatör özellikleri. Hacettepe Journal of Biology and Chemistry, Cilt: 39 - Sayı: 2, 157–162. https://ajindex.com/articles/529243

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (18)

  1. W. Kuo, J. Lin, T.H. Tang, Human glucose-6-phosphate dehydrogenase (G6PD) gene transforms NIH 3T3 cells and induces tumors in nude mice. Int. J. Cancer, 85 (2000) 857
  2. D.S. Shereve, H.R. Levy, On the molecular weight of human glucose 6- phosphate dehydrogenase. Biochem. Biophy. Com, 78 (1977) 1369
  3. A.L. Lehninger, D.L. Nelson, M.M. Cox, Principles of Biochemistry, 2nd Ed. New York: Worth Publishers Inc, (1993) 436
  4. I. Ozmen, O.I. Kufrevioglu, M. Gul, Investigation of effects of some antibiotics on activities of glucose 6-phosphate dehydrogenase and antioxidant enzymes in erythrocytes. Drug Chem. Toxicol, 28 (2005) 433
  5. M.I. Kanji, M.L. Toews, W.R. Carpar, A kinetic study of glucose-6-phosphate dehydrogenase. J. Biol. Chem, 251 (1976) 2258
  6. D. Bradley, FDA approval provides new option against breast cancer. Pharm. Sci. Technology, 2 (1999) 471
  7. C. Jacquin, X. Nasica, D. Guillard, O. Aujoulat, C. Stoeckel, Intravitreal treatment of endophthalmitis traitement intravitreen des endophtalmies. Médecine et Maladies Infectieuses, 31 (2001) 493
  8. A. Mehta, P.J. Mason, T.J. Vulliamy, Glucose-6- phosphate dehydrogenase deficiency. Best Pract. Res. Clin. Haematol, 13 (2000) 21
  9. N. Basaran, Medical Genetic. Istanbul: Gunes & Nobel Medical Press, (1983) 327
  10. M. Gul, F.Z. Kutay, S. Temocin, O. Hanninen, Cellular and clinical implications of glutathione. Indian J. Exp. Biol, 28 (2000) 625
  11. B. Heinzt, T.A. Bock, H. Kierdorf, N. Maurin, Haemolytic crisis after acetaminophen in glucose-6-phosphate dehydrogenase deficiency. Klin. Wochenschr, 67 (1989) 1068
  12. T. Meloni, G. Forteleoni, F. Ena, G.F. Meloni, Glucose- 6-phosphate dehydrogenase deficiency and bacterial infections in northern Sardinia. J. Pediatr, 118 (1991) 909
  13. A. Szeinberg, C. Sheba, N. Hirsborn, E. Bodonyi, Studies on erthrocytes in cases with past history of favism and drug-induced acute hemolytic anemia. Blood, 12 (1957) 603
  14. T. Honjo, A. Watanabe, Clinical experience on sulbactam/cefoperazone in the pediatric field. Jpn. J. Antibiot, 37 (1984) 32
  15. P.J. Dalgarno, D. Shewan, Illicit use of ketamine in Scotland. J. Psychoactive Drugs. 28 (1996) 191
  16. H.N. Wenk, M.N. Nannenga, C.N. Honda, Effect of morphine sulphate eye drops on hyperalgesia in the rat cornea. Pain, 105 (2003) 455
  17. S.O. Kayaalp, Rasyonel Tedavi Yönünden Tıbbi Farmakoloji. Ankara, Hacettepe-Tas Yayincilik, 2002
  18. P. Ninfali, T. Orsenigo, L. Barociani, S. Rapa, Rapid purification of glucose