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Journals / Hacettepe Journal of Biology and Chemistry / 2011 / Cilt: 39 - Sayı: 2

Cu2+-takılı magnetit nanopartikül gömülü süpermakrogözenekli monolitik kompozit kriyojellerin DNA adsorpsiyon uygulaması

Application of cu2+-attached magnetite nanoparticles embedded supermacroporous monolithic composite cryogels for DNA adsorption

Şeyda CEYLAN
Tülden KALBURCU
Mehmet GEDİKLİ
Mehmet ODABAŞI
Pages
163–172
Publication Date
2022-10-17
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The maximum amount of DNA adsorption was 19.97 mg/g polymer, at pH 8.0.

📌 3 Key Takeaways

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Objective & Hypothesis:Cryogels are known as a novel generation of stationary phases in the separation science, and they have been succesfully used as efficient adsorbents for separation and purification of biomolecules, such as proteins, enzymes, DNA, cell organelles and viruses in downstream processes.
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Key Findings & Data:In this study, DNA adsorption performance of Cu2+-attached magnetite (Fe3O4) nanoparticles (Cu2+-AMNPs) embedded supermacroporous monolithic composite cryogels were investigated.
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Conclusion & Impact:The maximum amount of DNA adsorption was 19.97 mg/g polymer, at pH 8.0.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Cryogels are known as a novel generation of stationary phases in the separation science, and they have been succesfully used as efficient adsorbents for separation and purification of biomolecules, such as proteins, enzymes, DNA, cell organelles and viruses in downstream processes. In this study, DNA adsorption performance of Cu2+-attached magnetite (Fe3O4) nanoparticles (Cu2+-AMNPs) embedded supermacroporous monolithic composite cryogels were investigated. Firstly, Magnetite nanoparticles (NPs) coated with 3-aminopropyltriethoxysilane (APTES) were prepared by silanization reaction. Then, Cu2+ ions were attached to silanized magnetite NPs through amine groups. After that, Cu2+-AMNPs were embedded into the cryogels for DNA adsorption studies. The prepared cryogel was characterized by scanning electron microscopy (SEM), elemental analysis, FTIR and swelling. SEM analysis indicates that the cryogel have a heteroporous structure with interconnected pores of 10-50 μm size, which ascribed to the porogens effect of frozen water crystals. The maximum amount of DNA adsorption was 19.97 mg/g polymer, at pH 8.0.

Özet

Kriyojeller ayırma biliminde yeni nesil sabit faz olarak değerlendirilmektedirler. Kriyojeller, altakım işlemlerinde protein, enzim, DNA, hücre organelleri ve virüsler gibi biyomoleküllerin ayrılması ve saflaştırılmasında etkin adsorbentler olarak kullanılmaktadır. Bu çalışmada, Cu2+-takılı magnetit (Fe3O4) nanopartikül (Cu2+-AMNP) gömülü süpermakrogözenekli monolitik kompozit kriyojellerin DNA adsorpsiyon performansı incelenmiştir. Öncelikle, 3-aminopropyltriethoxysilane (APTES) ile kaplanmış magnetit NP’ler silanizasyon reaksiyonu ile hazırlanmıştır. Daha sonra, silanlanmış magnetit NP’lere, amin grupları üzerinden Cu2+ iyonları takılmıştır. Bundan sonra, Cu2+-AMNP’ler DNA adsorpsiyon çalışmaları için süpermakrogözenekli monolitik kompozit kriyojellere gömülmüştür. Hazırlanan kriyojel taramalı elektron mikroskobu (SEM), elementel analiz, FTIR ve şişme testi ile karakterize edilmiştir. SEM analizleri kriyojellerin, donmuş su kristallerinin porojen etkisi ile oluşan birbirine bağlı 10-50 μm boyutunda gözenekleri ile heterogözenekli bir yapıya sahip olduğunu göstermiştir. Maksimum DNA adsorpsiyon miktarı pH 8’de 19.97 mg/g polymer olarak bulunmuştur.

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Cite This Article

CEYLAN, Ş., KALBURCU, T., GEDİKLİ, M., ODABAŞI, M. (2022). Cu2+-takılı magnetit nanopartikül gömülü süpermakrogözenekli monolitik kompozit kriyojellerin DNA adsorpsiyon uygulaması. Hacettepe Journal of Biology and Chemistry, Cilt: 39 - Sayı: 2, 163–172. https://ajindex.com/articles/529229

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