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Journals / Hacettepe Journal of Biology and Chemistry / 2018 / Cilt: 46 - Sayı: 1

Radiation Synthesis of Molecularly Imprinted Hydroxyethylmethacrylate-based Matrices for Glucose Recognition

Glikoz Tanıması için Hidroksietilmetakrilat Esaslı Moleküler Baskılı Matrislerin Radyasyon ile Sentezi

Olgun GÜVEN
Meshude AKBULUT SÖYLEMEZ
Pages
53–60
Publication Date
2022-10-17
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The average radii of cavities were found as 0.254 and 0.279 nm for freeze-dried imprinted polymers prepared by using PEG(200)DA after swollen in water and acetonitrile/water mixture (1/5 by volume), respectively.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, 2-hydroxyethyl methacrylate (HEMA) was used as functional monomer and diethylene glycol diacrylate (DEGDA) and polyethylene glycol (200) diacrylate (PEG(200)DA) were used as crosslinking agents to imprint D(+)glucose.
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Key Findings & Data:D(+)glucose imprinted polymers were prepared in the presence of dimethyl sulfoxide (DMSO) /isopropyl alcohol (IPA) (3/1, v/v) at room temperature, in the air by radiation-induced polymerization/ crosslinking.
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Conclusion & Impact:The average radii of cavities were found as 0.254 and 0.279 nm for freeze-dried imprinted polymers prepared by using PEG(200)DA after swollen in water and acetonitrile/water mixture (1/5 by volume), respectively.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, 2-hydroxyethyl methacrylate (HEMA) was used as functional monomer and diethylene glycol diacrylate (DEGDA) and polyethylene glycol (200) diacrylate (PEG(200)DA) were used as crosslinking agents to imprint D(+)glucose. D(+)glucose imprinted polymers were prepared in the presence of dimethyl sulfoxide (DMSO) /isopropyl alcohol (IPA) (3/1, v/v) at room temperature, in the air by radiation-induced polymerization/ crosslinking. The control polymers were synthesized by the same procedure in the absence of D(+)glucose. In order to evaluate the recognition and separation properties of the imprinted system high performance liquid chromatography (HPLC) experiments were carried out where β (-) lactose, D(+)glucose and glycerol were used as analytes. To increase the affinity of the template to the stationary phase polarity of the mobile phase was decreased by the addition of acetonitrile into water. Optimum composition of acetonitrile/water (1/5 v/v) was determined according to the swelling experiments. The sizes of the cavities in the polymeric networks were determined by positron annihilation lifetime spectroscopy (PALS). The average radii of cavities were found as 0.254 and 0.279 nm for freeze-dried imprinted polymers prepared by using PEG(200)DA after swollen in water and acetonitrile/water mixture (1/5 by volume), respectively.

Özet

Bu çalışmada D (+) Glikoz baskılamak amacıyla fonksiyonel monomer olarak 2-hidroksietil metakrilat (HEMA), çapraz bağlayıcı olarak dietilenglikol diakrilat (DEGDA) ve polietilenglikol (200) diakrilat (PEG(200)DA) kullanılmıştır. D (+) Glikoz baskılı polimerler dimetil sülfoksit (DMSO)/izopropil alkol (İPA) (hacimce, 3/1) varlığında oda sıcaklığında, havada radyasyonla başlatılan polimerizasyon/çapraz bağlanma ile hazırlanmıştır. Kontrol polimerleri D (+) glikoz yokluğunda aynı reçete ile sentezlenmiştir. Baskılı sistemin tanıma ve ayırma özelliklerini değerlendirmek amacıyla β (-) laktoz, D (+) glikoz and gliserolün analit olarak kullanıldığı yüksek performanslı sıvı kromatografisi (HPLC) deneyleri yapılmıştır. Hedef molekülün durgun faza olan ilgisini arttırmak amacıyla suya asetonitril eklenerek hareketli fazın polaritesi azaltılmıştır. Asetonitril/su (1/5, v/v) için optimum bileşim şişme deneylerine göre belirlenmiştir. Polimerik ağ yapı içindeki boşlukların boyutları positron yok olma ömrü spektroskopisi (PALS) ile hesaplanmıştır. PEG(200)DA kullanılarak hazırlanan suda ve asetonitril/su (hacimce, 1/5) karışımında şişirildikten sonra dondurularak kurutulmuş baskılı polimerlerin ortalama yarıçapları sırasıyla 0.254 ve 0.279 nm olarak hesaplanmıştır.

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Cite This Article

GÜVEN, O., SÖYLEMEZ, M. A. (2022). Radiation Synthesis of Molecularly Imprinted Hydroxyethylmethacrylate-based Matrices for Glucose Recognition. Hacettepe Journal of Biology and Chemistry, Cilt: 46 - Sayı: 1, 53–60. https://ajindex.com/articles/529074

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