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Journals / Hacettepe Journal of Biology and Chemistry / 2018 / Cilt: 46 - Sayı: 4

Impedimetric Nanobiosensor for the Detection of Sequence-Selective DNA Hybridization

Dizi Seçimli DNA Hibridizasyonu için İmpedimetrik Nanobiyosensör

Merve MUTİ
Arzum ERDEM
Mihrican MUTİ
Pages
495–503
Publication Date
2022-10-17
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The detection limit was calculated as 0.86 µg/mL.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:5-amino-2-mercapto-1,3,4-thidiazole (AMT) enriched gold nanoparticles (AuNPs) modified impedimetric sensors were developed for the electrochemical monitoring of sequence-selective DNA hybridization related to Hepatitis B virus (HBV).
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Key Findings & Data:AMT-AuNP-PGEs presented more repeatable impedimetric responses and provided a suitable surface for more DNA binding onto AMT-AuNPs-PGE surface.
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Conclusion & Impact:The detection limit was calculated as 0.86 µg/mL.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

5-amino-2-mercapto-1,3,4-thidiazole (AMT) enriched gold nanoparticles (AuNPs) modified impedimetric sensors were developed for the electrochemical monitoring of sequence-selective DNA hybridization related to Hepatitis B virus (HBV). AMT-AuNP-PGEs presented more repeatable impedimetric responses and provided a suitable surface for more DNA binding onto AMT-AuNPs-PGE surface. The selectivity of DNA biosensor modified with AMT-AuNP was investigated in the presence of target DNA, or the other DNA sequences; e.g, noncomplementary (NC), or mismatch (MM) DNA sequences. The detection limit was calculated as 0.86 µg/mL.

Özet

Altın nanopartiküller (AuNPs) ile zenginleştirilmiş 5-amino-2-mercapto-1,3,4-thidiazole (AMT) modifiye impedimetrik sensörler, Hepatit B virüsüne (HBV) ilişkin dizi seçimli DNA hibridizasyonunun elektrokimyasal olarak izlenmesi için geliştirilmiştir. AMT-AuNP-PGE ler daha fazla miktarda DNA’nın AMT-AuNPs-PGE yüzeyine bağlanması için uygun bir yüzey sağlamış ve daha tekrarlanabilir impedimetrik sinyaller vermiştir. AMT-AuNP ile modifiye edilmiş DNA biyosensörlerin seçimliliği, hedef DNA, veya diğer DNA dizileri; rastgele dizi (NC), mutasyonlu dizi (MM) varlığında test edilmiştir. Tayin sınırı 86 µg/mL olarak hesaplanmıştır.

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Cite This Article

MUTİ, M., ERDEM, A., MUTİ, M. (2022). Impedimetric Nanobiosensor for the Detection of Sequence-Selective DNA Hybridization. Hacettepe Journal of Biology and Chemistry, Cilt: 46 - Sayı: 4, 495–503. https://ajindex.com/articles/529060

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