Journals / Hacettepe Journal of Biology and Chemistry / 2018 / Cilt: 46 - Sayı: 1

Trichoderma citrinoviride: A Potent Biopriming Agent for the Alleviation of Salt Stress in Maize

Trichoderma citrinoviride: Mısırda Tuz Stresinin Azaltılmasında Kullanılabilecek Potansiyel Bir Ön Muamele Ajanı

Pages
101–111
Publication Date
2022-10-17
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Our ongoing research is directed toward developing powdered fungal biopreperations to assay multiple stress tolerances in agriculture for agroeconomically important cereals such as maize.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The rapid increase in global population and industrial pollution pose severe environmental threats to agriculture that are exacerbated by salt stress.
📊
Key Findings & Data:Genetic lineages of fungi were identified using well-known fungal ITS (internal transcribed spacer) barcodes that revealed similarity to the Trichoderma citrinoviride (T11C) species.
💡
Conclusion & Impact:Our ongoing research is directed toward developing powdered fungal biopreperations to assay multiple stress tolerances in agriculture for agroeconomically important cereals such as maize.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The rapid increase in global population and industrial pollution pose severe environmental threats to agriculture that are exacerbated by salt stress. Molecular characterization of new fungal isolates and assessment of their impact on agriculture might be an eco-friendly approach to modulating salt tolerance. Herein, fungal seed biopriming was conducted on salt (NaCl) stressed maize in a dose-dependent manner. Genetic lineages of fungi were identified using well-known fungal ITS (internal transcribed spacer) barcodes that revealed similarity to the Trichoderma citrinoviride (T11C) species. Fv/Fm, ETR and qP were recorded as close to optimum in bioprimed maize plants after application of salt stress. NPQ (nonphotochemical quenching) decreased slightly in respective groups. Higher photosynthetic pigment contents were also detected. T11C seed biopriming decreased the lipid oxidation remarkably under salt stress. SOD, GPX, GR and CAT activities were not found to be significantly induced in the roots or leaves of T11C after biopriming. However, higher RWC (relative water content), soluble protein and proline were measured in bioprimed test groups treated with high salt stress, demonstrating increased osmoregulatory capacity. Our ongoing research is directed toward developing powdered fungal biopreperations to assay multiple stress tolerances in agriculture for agroeconomically important cereals such as maize.

Özet

Küresel nüfus artışı ve endüstriyel kirlilik, tuz stresi ile birleştiğinde, tarımsal şartları ağırlaştıran çevresel tehditlere dönüşmektedir. Yeni fungal izolatların moleküler karakterizasyonu ve bunların tarım üzerindeki etkilerinin araştırılması, tuz stresi toleransının kontrolünde çevre dostu bir yaklaşım olabilir. Bu bağlamda mevcut çalışmada, doza bağımlı tuz (NaCl) stresi uygulanan mısır tohumlarına fungal ön muamele gerçekleştirilmiştir. Fungusun genetik bağlantıları iyi bilinen ITS barkodları kullanılarak tanımlanmış ve Trichoderma citrinoviride (T11C) türüne benzerlik gösterdiği ortaya çıkarılmıştır. Fungal uygulamanın yapıldığı stres altındaki mısır bitkilerinde, maksimum kuantum verimi (Fv/Fm), elektron transfer oranı (ETO), ve fotokimyasal kullanılmanın (qP) optimuma yakın olduğu, bununla birlikte NPQ (fotokimyasal olmayan floresans sönmesi) değerlerinin ise; ilgili gruplarda azaldığı belirlenmiştir. Ayrıca, fotosentetik pigment içeriklerinin yüksek olduğu kaydedilmiştir. T11C fungal muamelesinin, tuz stresi altındaki bitkilerde lipid oksidasyonunu düşürdüğü tespit edilmekle birlikte, mısır kök ve yapraklarında SOD, GPX, GR ve CAT aktivitelerini belirgin şekilde arttırmadığı gözlenmiştir. Yüksek tuz stresi uygulanan test gruplarında, yüksek RWC (göreceli su içeriği), çözünebilir protein ve prolinin tespiti, fungal ön muamelenin, bitkilerde artan bir osmoregülasyon kapasitesini tetiklediğini kanıtlamıştır. Devam eden çalışmalar, mısır gibi ekonomik açıdan önemli tahıllarda çoklu stres toleranslarını test etmek adına, tarımsal uygulamalarda kullanılabilecek toz halindeki mantar biyopreparatlarının geliştirilmesine yöneliktir.

🔖

Cite This Article

PEHLİVAN, N., DURMUŞ, N., KARAOĞLU, S. A., YEŞİLYURT, A. M. (2022). Trichoderma citrinoviride: A Potent Biopriming Agent for the Alleviation of Salt Stress in Maize. Hacettepe Journal of Biology and Chemistry, Cilt: 46 - Sayı: 1, 101–111. https://ajindex.com/articles/529048

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (12)

  1. F. Moradi, A.M. Ismail, Responses of photosynthesis, chlorophyll fluorescence and ROS-scavenging systems to salt stress during seedling and reproductive stages in rice, Ann Bot., 99 (2007) 1161-1173.
  2. K. Maxwell, GN. Johnson, Chlorophyll fluorescence-a practical guide, J. Exp. Bot, 51 (2000) 659-668.
  3. S.E. Ahmed, M.H. Nawata, Y. Domae, T. Sakuratani, Alterations in photosynthesis and some antioxidant enzymatic activities of mungbean subjected to waterlogging, Plant Sci., 163 (2002) 117-123.
  4. A.F. Lodeyro, M. Giró, H.O. Poli, G. Bettucci, A. Cortadi, A.M Ferri, N. Carrillo, Suppression of reactive oxygen species accumulation in chloroplasts prevents leaf damage but not growth arrest in salt-stressedtobacco plants. PLoS One, 11 (2016). http://doi.org/10.1371/ journal.pone.0159588 N. Pehlivan, A.M. Yesilyurt, N. Durmus, S.A. Karaoglu, Trichoderma lixii ID11D seed biopriming mitigates dose dependent salt toxicity in maize, Acta Physiol. Plant., (2017) 10.1007/s1738-017-2375-z. R. Mittler, Oxidative stress, antioxidants and stress tolerance, Trends in Plant Sci., 7 (2002) 405-410.
  5. R.K. Behera, PC. Mishra, N.K. Choudhary, High irradiance and water stress induced alterations in pigment composition and chloroplast activities of primary wheat leaves, J. Plant Physiol., 159 (2002) 967-973.
  6. M. Eisendle, H. Oberegger, R. Buttinger, P. Illmer, H. Haas Biosynthesis and uptake of siderophores is controlled by the PacC-mediated ambient-pH Regulatory system in Aspergillus nidulans, Eukaryot. Cell, 3 (2004) 561-563.
  7. R. Hermosa, L. Botella, M. Montero-Barrientos, A. Alonso-Ramirez, V. Arbona, A. Gomez-Cadenas, C. Nicolas, Biotechnological applications of the gene transfer from the beneficial fungus Trichoderma harzianum to plants, Plant Signal. Behav., 6 (2011) 1235-1236.
  8. G.E. Harman, Myths and dogmas of biocontrolchanges in perceptions derived from research on Trichoderma harzianum T-22, Plant Disease, 84 (2000) 377-393.
  9. T. Benitez, A.M. Rincon, M.C. Limon, A.C. Codon, Biocontrol mechanisms of Trichoderma strains, Int. Microbiol., 7 (2004) 249-260.
  10. C.H. Foyer, B. Halliwell, Presence of glutathione and glutathione reductase in chloroplast: a proposed role in ascorbic acid metabolism, Planta, 133 (1976) 21-25.
  11. H. Aebi, Catalase. In: Bergmeyer H, editor. Methods of Enzymatic Analysis. 3rd ed. Weinheim, Germany: Verlag Chemie, (1983) 273-286.
  12. H. Urbanek, E. Kuzniak-Gebarowska, K. Herka, Elicitation of defense responses in bean leaves by Botrytis cinerea polygalacturanase, Acta. Ph