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Journals / Hacettepe Journal of Biology and Chemistry / 2020 / Cilt: 48 - Sayı: 1

A HILIC Stationary Phase Functionalized with Glutathione by Thiol-Ene Chemistry on Monodisperse-Porous Polymer Microparticles

Monodispers-Gözenekli Polimer Mikropartiküller Üzerinde Tiyol-En Kimyası Kullanılarak Glutatyon ile Fonksiyonelleştirilmiş HILIC Sabit Fazı

Çiğdem KİP
Pages
21–31
Publication Date
2022-10-17
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results showed the usability of tailored poly (TMSPM-co-EDMA)microparticles as a stationary phase and thiol-ene chemistry in the manufacture of a chromatographic stationary phasewith high efficiency in hydrophilic liquid chromatography applications.

📌 3 Key Takeaways

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Objective & Hypothesis:Monodisperse-porous microparticles functionalized with glutathione by thiol-ene chemistry were synthesized as a newstationary phase for hydrophilic interaction chromatography.
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Key Findings & Data:Monodisperse-porous poly(3-trimethoxysilylpropylmethacrylate-co-ethylene dimethacrylate), poly(TMSPM-co-EDMA) microspheres (6 μm in size) were obtained with different seed latex/monomer ratios and diluent compositions by multistage microsuspension copolymerization.
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Conclusion & Impact:The results showed the usability of tailored poly (TMSPM-co-EDMA)microparticles as a stationary phase and thiol-ene chemistry in the manufacture of a chromatographic stationary phasewith high efficiency in hydrophilic liquid chromatography applications.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Monodisperse-porous microparticles functionalized with glutathione by thiol-ene chemistry were synthesized as a newstationary phase for hydrophilic interaction chromatography. Monodisperse-porous poly(3-trimethoxysilylpropylmethacrylate-co-ethylene dimethacrylate), poly(TMSPM-co-EDMA) microspheres (6 μm in size) were obtained with different seed latex/monomer ratios and diluent compositions by multistage microsuspension copolymerization. The zwitterionic chromatographic ligand containing thiol moiety (i.e. glutathione) were covalently attached onto the TMSPM attachedpoly(TMSPM-co-EDMA) microparticles. The selected starting material allowed the direct attachment of zwitterionic ligandonto the support material using thiol-ene chemistry. The derivatized microparticles were slurry packed into the microborecolumns with 2 mm i.d. and used as stationary phase for the separation of nucleosides in hydrophilic interaction chromatography with the plate numbers up to 54.000 plates/m. The results showed the usability of tailored poly (TMSPM-co-EDMA)microparticles as a stationary phase and thiol-ene chemistry in the manufacture of a chromatographic stationary phasewith high efficiency in hydrophilic liquid chromatography applications.

Özet

Tiyol-en kimyası kullanılarak glutatyon ile fonksiyonelleştirilmiş eşboyutlu-gözenekli mikropartiküller, hidrofilik etkileşim kromatografisi için yeni bir sabit faz olarak sentezlenmiştir. Monodispers-gözenekli poli (3-trimetoksisililpropilmetakrilatco-etilendimetakrilat), poli(TMSPM-co-EDMA) mikropartiküller (boyut olarak 6 mikron), farklı çıkış lateksi/monomer ve diluent bileşimleri ile çok basamaklı mikrosüspansiyon kopolimerizasyonu ile elde edilmiştir. Tiyol ucu içeren zwitterionik kromatografik ligand (glutatyon), TMSPM bağlı poli (TMSPM-co-EDMA) mikropartiküller üzerine kovalent olarak bağlanmıştır. Seçilen başlangıç materyali, zwitteriyonik kromatografik ligandın, tiyolen kimyası kullanılarak destek materyali üzerine doğrudan bağlanmasına olanak sağlamıştır. Türevlendirilmiş mikropartiküller, 2 mm iç çapa sahip mikrobor kolonlar içerisine dolgulanmış ve hidrofilik etkileşim kromatografisinde 54000 plaka/m plaka sayısı ile nükleositlerin ayrılması için sabit faz olarak kullanılmıştır. Sonuçlar, yüksek verimlilikle hidrofilik sıvı kromatografisi uygulamaları için kromatografik sabit fazın üretiminde poli(TMSPM-co-EDMA) mikropartiküllerin ve tiyol-en kimyasının kullanılabileceğini göstermiştir.

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Cite This Article

KİP, Ç. (2022). A HILIC Stationary Phase Functionalized with Glutathione by Thiol-Ene Chemistry on Monodisperse-Porous Polymer Microparticles. Hacettepe Journal of Biology and Chemistry, Cilt: 48 - Sayı: 1, 21–31. https://ajindex.com/articles/528987

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References (12)

  1. A.J. Alpert, Hydrophilic-Interaction chromatography for the separation of peptides, nucleic-Acids and other polar compounds, J. Chromatogr., 499 (1990) 177-196
  2. T. Ikegami, K. Tomomatsu, H. Takubo, K. Horie, N. Tanaka, Separation efficiencies in hydrophilic interaction chromatography, J. Chromatogr. A, 1184 (2008) 474-503
  3. Y. Guo, S. Gaiki, Retention behavior of small polar compounds on polar stationary phases in hydrophilic interaction chromatography, J. Chromatogr. A, 1074 (2005) 71-80 4. A.R. Oyler, B.L. Armstrong, J.Y. Cha, M.X. Zhou, Q. Yang, R.I. Robinson, R. Dunphy, D.J. Burinsky, Hydrophilic-Interaction Chromatography on Amino-Silica Phases Complements Reversed-Phase High Performance Liquid Chromatography and Capillary Electrophoresis for Peptide Analysis, J. Chromatogr. A., 724 (1996) 378-383
  4. T.J. Yoshida, Peptide separation by Hydrophilic-Interaction Chromatography: a review, Biochem Biophys Meth., 60 (2004) 265–280
  5. Z.G. Hao, C. Lu, B.M. Xiao, N.D. Wenig, B. Parker, M. Knapp, C. Ho, Separation of amino acids, peptides and corresponding Amadori compounds on a silica column at elevated temperature, J. Chromatogr. A., 1147 (2007) 165-171
  6. T. Ikegami, K. Tomomatsu, H. Takubo, K. Horie, N. Tanaka, Separation efficiencies in hydrophilic interaction chromatography, J. Chromatogr. A., 1184 (2008) 474-503
  7. S. Cubbon, T. Bradbury, J. Wilson, J. Thomas-Oates, Hydrophilic interaction chromatography for mass spectrometric metabonomic studies of urine, Anal Chem., 79 (2007) 8911-8918
  8. B. Buszewski, S. Noga, Hydrophilic interaction liquid chromatography (HILIC)—a powerful separation technique, Anal. Bioanal. Chem., 402 (2012) 231-247
  9. B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts, P. Walter, Molecular biology of the cell, New York: Garland Science, 2002
  10. N. Kochanowski, F. Blanchard, R. Cacan, F. Chirat, E. Guedon, A. Marc, J.L. Goergen, Intracellular nucleotide and nucleotide sugar contents of cultured CHO cells determined by a fast, sensitive, and high-resolution ion-pair RP-HPLC, Anal. Biochem., 348 (2006) 243-251
  11. P. Yeung, L. Ding, W.L. Casley, HPLC assay with UV detection for determination of RBC purine nucleotide concentrations and application for biomarker study in vivo, J. Pharm. Biomed. Anal., 47 (2008) 377-382
  12. X. Lv, W. Tan, Y. Chen, Y. Chen, M. Ma, B. Chen, S. Yao, Facile “one-pot” synthesis of poly(methacrylic acid)-based hybridmonolith via thiol-ene click reaction for hydrophilic interactionchrom