AI Summary & Key Takeaways
AI InsightThe commercial AgNPs also displayed lower antifungal activity than their green equivalents synthesized in this research, and even a low (~50 μg/mL) concentration of synthesized AgNPs was found to be effective in suppressing the growth of Fusarium tricinctum and Colletotrichum sp.
📌 3 Key Takeaways
Abstract
The aim of this study was to synthesize silver nanoparticles (AgNPs) using leaf, root, and stem extracts of Phaseolus vulgaris L. (Yunus-90) and elucidate their antifungal activities. In this regard, the prepared AgNPs have been characterized by using UV-vis, FT-IR, TEM, SEM, and DLS techniques. Then, the antifungal activity of both synthesized and commercially purchased AgNPs was investigated via (i) agar well diffusion, (ii) fungal colony morphotype diversity, (iii) inhibition of hyphae and (iv) minimum inhibition concentration (MIC) analyses against Colletotrichum sp., Fusarium oxysporum, Fusarium acuminatum, Fusarium tricinctum, Fusarium graminearum, Fusarium incarnatum, Rhizoctonia solani, Sclerotinia sclerotiorum, and Alternaria alternata. The AgNPs derived from the leaf extract displayed significantly higher levels of antifungal activity relative to the AgNPs prepared from the root and stem extracts. The commercial AgNPs also displayed lower antifungal activity than their green equivalents synthesized in this research, and even a low (~50 μg/mL) concentration of synthesized AgNPs was found to be effective in suppressing the growth of Fusarium tricinctum and Colletotrichum sp.
Özet
Bu çalışmanın amacı, Phaseolus vulgaris L.’nin (Yunus-90) yaprak, kök ve gövde özütlerini kullanarak gümüşnanopartiküllerini (AgNPs) sentezlemek ve antifungal aktivitelerini ölçmektir. Bu bağlamda, hazırlanan AgNP’ler UV- vis, FT-IR, TEM, SEM ve DLS teknikleri kullanılarak karakterize edilmiştir. Ardından hem sentezlenen hem de ticari olaraksatın alınan AgNP’lerin Colletotrichum sp., Fusarium oxysporum, Fusarium acuminatum, Fusarium tricinctum, Fusariumgraminearum, Fusarium incarnatum, Rhizoctonia solani, Sclerotinia sclerotiorum ve Alternaria alternata’ya karşı antifungalaktivitesi; (i) agar kuyu difüzyonu, (ii) mantar kolonisinin morfolojik çeşitliliği, (iii) hif inhibisyonu ve (iv) minimum inhibisyonkonsantrasyonu (MIC) analizleri ile araştırılmıştır. Yaprak özütlerinden türetilen AgNP’ler, kök ve gövde özütlerindenhazırlanan AgNP’lere göre önemli ölçüde yüksek antifungal aktivite sergilemiştir. Ticari AgNP’ler ayrıca bu araştırmadasentezlenen yeşil eşdeğerlerinden daha düşük antifungal aktivite göstermiştir. Ayrıca, sentezlenen AgNP’lerin düşük (~ 50 μg/ mL) konsantrasyonunun bile Fusarium tricinctum ve Colletotrichum sp’nin gelişimini baskılamada etkili olduğu bulunmuştur.