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Journals / Hacettepe Journal of Biology and Chemistry / 2022 / Cilt: 50 - Sayı: 1

CHEMICAL CHAPERONE EFFECTS ON ARGININE-VASOPRESSIN RECEPTOR 2 MUTANTS

Tuğçe KARADUMAN
Emel SAĞLAR ÖZER
Beril ERDEM TUNÇDEMİR
Hatice MERGEN
Dilara ŞAHİN
Merve ÖZCAN TÜRKMEN
Pages
37–43
Publication Date
2022-10-17
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

We may conclude that such chaperone treatment studies are valuable for development of the therapeutic strategies.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Improper folding of the mutant proteins may finally cause several conformational diseases such as Nephrogenic Diabetes Insipidus (NDI).
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Key Findings & Data:In recent years, as a therapeutic strategy, chaperone treatment for such diseases is among current issues.
💡
Conclusion & Impact:We may conclude that such chaperone treatment studies are valuable for development of the therapeutic strategies.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Chaperone · AVPR2 · NDI

Abstract

Improper folding of the mutant proteins may finally cause several conformational diseases such as Nephrogenic Diabetes Insipidus (NDI). In recent years, as a therapeutic strategy, chaperone treatment for such diseases is among current issues. In our study, we aimed to analyze the effect of several chemical chaperones on mutant V2 receptors which cause NDI. V2R mutant constructs were introduced into the pLV2R. Mutants were transiently expressed in COS-7 cells. After MTT analyses, cell surface ELISA experiment was performed for understanding the rescue potential of the chaperones of the mutated proteins. As a result, we analyzed that rescue potential of a chemical chaperone depends on both chemical compound and the mutation type. We may conclude that such chaperone treatment studies are valuable for development of the therapeutic strategies.

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Cite This Article

KARADUMAN, T., ÖZER, E. S., TUNÇDEMİR, B. E., MERGEN, H., ŞAHİN, D., TÜRKMEN, M. Ö. (2022). CHEMICAL CHAPERONE EFFECTS ON ARGININE-VASOPRESSIN RECEPTOR 2 MUTANTS. Hacettepe Journal of Biology and Chemistry, Cilt: 50 - Sayı: 1, 37–43. https://ajindex.com/articles/528901

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