Journals / Gazi Üniversitesi Diş Hekimliği Fakültesi Dergisi / 2008 / Cilt: 25 - Sayı: 3

The effect of different polymerization modes on the cytotoxicity of ormocer based resin composite, in vitro

Farklı polimerizasyon fiekillerinin ormoser esaslı rezin restoratif materyalin sitotoksisitesi üzerine etkisi, in vitro

Pages
31–37
Publication Date
2022-10-17
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As the light intensity increases, the cytotoxic effects of the material were decreased.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The aim of this study was to evaluate the effects of three different light exposure modes on in vitro toxicity of ormocer-based resin composite (Admira, Voco).
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Key Findings & Data:Material and Method: The specimens were cured with halogen light curing unit in teflon moulds of 2 mm depth and 10 mm diameter.
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Conclusion & Impact:As the light intensity increases, the cytotoxic effects of the material were decreased.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The aim of this study was to evaluate the effects of three different light exposure modes on in vitro toxicity of ormocer-based resin composite (Admira, Voco). Material and Method: The specimens were cured with halogen light curing unit in teflon moulds of 2 mm depth and 10 mm diameter. The modes adjusted were conventional light curing, ramp curing and boost. The glass slides in the same dimentions were used as negative control. Polymerized specimens and negative controls were immersed into Dulbeccos’ Modified Eagle’s Medium (DMEM F12) for 24 and 48 hours and then the extracts were collected. Afterwards L 929 fibroblast-like cells were incubated for 24, 48 and 72 h in 100 μl of these extracts. Cellular activity was assessed with MTT test. One way analyses of variance (ANOVA) and paired samples t-test were used for statistical analyses. Result: There were no statistically significant difference between the test groups and the negative control group after 24, 48 and 72 h with both 24 and 48 h extracts (p0.05). Conclusion: Either polymerized with one of the three polymerization techniques ormocer based composite resin Admira have some cytotoxic effects on L 929 mouse fibroblast cell cultures. As the light intensity increases, the cytotoxic effects of the material were decreased.

Özet

Amaç: Ormoser esaslıbir rezin restoratif materyalin (Admira, Voco) polimerizasyonunda halojen ışık kaynağının üç farklıayarda (geleneksel, boost, ramp) kullanılmasının, materyalin sitotoksisitesi üzerine etkilerinin in vitro olarak incelenmesidir. Gereç ve Yöntem: 10 mm çapında ve 2 mm derinliğinde teşon kalıplar içerisinde hazırlanan kompozit rezin örnekler halojen ışık kaynağının geleneksel, boost veya ramp ayarlarıkullanılarak polimerize edildi. Aynıbüyüklüklerdeki disk şeklinde kesilmiş cam parçalarınegatif kontrol grubu olarak kullanıldı. Polimerize edilmiş örnekler ve negatif kontrol grubu örnekleri Dulbeccos’ Modified Eagles Medium (DMEM F12) içerisinde 24 ve 48 saat bekletildikten sonra ekstreleri toplandı. L 929 fibroblast benzeri hücreler 24, 48 ve 72 saat süresince bu ekstrelerin 100 μl’sinde inkübe edildi. Hücresel aktivite, MTT testi kullanılarak saptandı. ‹statistiksel analizde tek yönlü varyans analizi (ANOVA) ve eşleştirilmiş t-testi kullanıldı. Bulgular: 24 ve 48 saatlik ekstrelerin her ikisinin de 24, 48 ve 72 saatlik inkübasyonlarından sonra negatif kontrol gözlerinin MTT ortalamalarıve deney gruplarının tümünün (geleneksel, ramp ve boost) MTT ortalamalarıarasında istatistiksel olarak anlamlıfark gözlendi (p0.05). Sonuç: Ormoser esaslıkompozit rezin Admira, incelenen her üç polimerizasyon şekliyle de L 929 fare fibroblast hücreleri üzerinde farklıderecelerde sitotoksik etki meydana getirmektedir. Işık yoğunluğunun artmasısitotoksik etkinin azalmasına neden olmaktadır.

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Cite This Article

BALA, O., ARISU, D. H., TÜRKÖZ, E., YILMAZ, Ş. (2022). The effect of different polymerization modes on the cytotoxicity of ormocer based resin composite, in vitro. Gazi Üniversitesi Diş Hekimliği Fakültesi Dergisi, Cilt: 25 - Sayı: 3, 31–37. https://ajindex.com/articles/521556

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