Journals / Eurasian Journal of Veterinary Sciences / 2018 / Cilt: 34 - Sayı: 3

Phenotypic and molecular identification of extended-spectrum beta-lactamases in Escherichia coli in healthy broilers at the west region of Iran

İran’ın batı bölgesinde sağlıklı broylerlerdeki Escherichia coli’lerde geniş spektrumlu beta laktamazların fenotipik ve moleküler identifikasyonu

Pages
178–184
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Consistentmonitoring and proper use of antibiotics in the region’s poultryindustry should be applied.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: The presence of fecal carriers of extended-spectrum beta-lactamase (ESBL) genes in poultry is worrisome because itis likely to spread to a human being.
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Key Findings & Data:This study was proposedto estimate the frequency of ESBLs carriers in Escherichia coliisolated from healthy broiler’s gut in west of Iran.Materials and Methods: A total of 300 fecal samples were collectedfrom apparently healthy broiler.
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Conclusion & Impact:Consistentmonitoring and proper use of antibiotics in the region’s poultryindustry should be applied.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: The presence of fecal carriers of extended-spectrum beta-lactamase (ESBL) genes in poultry is worrisome because itis likely to spread to a human being. This study was proposedto estimate the frequency of ESBLs carriers in Escherichia coliisolated from healthy broiler’s gut in west of Iran.Materials and Methods: A total of 300 fecal samples were collectedfrom apparently healthy broiler. E. coli strains were isolated,confirmed molecularly and studied using disk combinationmethod, antimicrobial susceptibility test and ESBL-detectionpolymerase chain reactions (PCR).Results: Two hundred and eighty five strains of E. coli wereisolated biochemically and all were confirmed in a speciesspecificPCR reaction. Antimicrobial susceptibility test on 156ESBL-producing E. coli identified by phenotypic method revealedthat 100 % of them were resistant to at least one antibiotic,while all were sensitive to imipenem. The prevalence of blaTEM,blaCTX-M, and blaSHV genes was 52.63%, 38.59%, and 30.17%respectively. The simultaneous existence of several ESBL geneswas identified in 52.61% of the isolates.Conclusions: The vast prevalence of ESBL carriers with multipleresistance in poultry is a threat to public health. Consistentmonitoring and proper use of antibiotics in the region’s poultryindustry should be applied.

Özet

Amaç: İnsana yayılma olasılığı yüksek olduğu için kümes hayvanlarında genişlemiş spektrumlu beta-laktamaz (GSBL) genlerinin fekal taşıyıcılarının varlığı endişe verici bir durumdur. Bu çalışma, İran'ın batısında sağlıklı broyler bağırsağından izole edilen Escherichia coli'deki ESBL taşıyıcılarının sıklığını tahmin etmek için yapılmıştır. Gereç ve Yöntem: Görünüş olarak sağlıklı broilerden toplam 300 dışkı örneği toplandı. E. coli suşları izole edildi, moleküler olarak doğrulandı ve disk kombinasyon yöntemi, antimikrobiyal duyarlılık testi ve ESBL-saptama polimeraz zincir reaksiyonları (PZR) kullanılarak incelendi. Bulgular: 285 E. coli suşu, biyokimyasal olarak izole edildi ve hepsi, türe özgü bir PZR reaksiyonuyla doğrulandı. Fenotipik yöntemle saptanan 156 ESBL üreten E. coli üzerinde antimikrobiyal duyarlılık testi, bunların % 100'ünün en az bir antibiyotiğe dirençli olduğunu, bunların hepsinin de imipeneme duyarlı olduğunu ortaya koymuştur. BlaTEM, blaCTX-M ve blaSHV genlerinin prevalansı sırasıyla% 52.63,% 38.59 ve% 30.17 idi. Birkaç ESBL geninin eşzamanlı varlığı, izolatların % 52.61'inde tespit edilmiştir. Öneri: Kümes hayvanlarında çoklu dirençli ESBL taşıyıcılarının yaygınlığı, halk sağlığına yönelik bir tehdittir. Bölgedeki kümes hayvanları endüstrisinde tutarlı izleme ve uygun antibiyotik kullanımı uygulanmalıdır.

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Cite This Article

AHMADI, E., SHEIKHI, S. (2022). Phenotypic and molecular identification of extended-spectrum beta-lactamases in Escherichia coli in healthy broilers at the west region of Iran. Eurasian Journal of Veterinary Sciences, Cilt: 34 - Sayı: 3, 178–184. https://ajindex.com/articles/509899

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