Journals / Eurasian Journal of Veterinary Sciences / 2022 / Cilt: 38 - Sayı: 1

Occurrence and antibiotic susceptibility of Vibrio spp., Aeromonas spp. and Listeria spp. in seafoods

Deniz ürünlerinde Vibrio spp., Aeromonas spp. ve Listeria spp.'nin varlığı ve antibiyotik duyarlılıkları

Pages
7–16
Publication Date
2022-10-18
DOI
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Conclusion: As a result, it is thought that fish and shrimp consumed in Turkey may be contaminated with pathogenic Vibrio and Aeromonas species, and antibiotic-resistant isolates may pose a risk to public health.

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Objective & Hypothesis:Aim: In this study, it was aimed to determine the presence of Vibrio spp., Aeromonas spp., and Listeria spp. in finned fish and shrimps consumed in Turkey and the antibiotic resistance profile of the isolates using disc diffusion method.
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Key Findings & Data:Materials and Methods: In the research, 300 seafoods obtained at different times were used as material.
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Conclusion & Impact:Conclusion: As a result, it is thought that fish and shrimp consumed in Turkey may be contaminated with pathogenic Vibrio and Aeromonas species, and antibiotic-resistant isolates may pose a risk to public health.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: In this study, it was aimed to determine the presence of Vibrio spp., Aeromonas spp., and Listeria spp. in finned fish and shrimps consumed in Turkey and the antibiotic resistance profile of the isolates using disc diffusion method. Materials and Methods: In the research, 300 seafoods obtained at different times were used as material. Following isolation by classical cultural method, classical PCR was performed to confirm the isolates at genus level and to identify at species level for pathogenic species. Results: Finfish and shrimp samples were contaminated with Vibrio spp., Aeromonas spp. and Listeria spp. with the rate of 19.4% (33/170), 14.7% (25/170), 4.1% (7/170) and 13.8% (18/130), 13.1% (17/130), 6.2% (8/130), respectively. Twenty-nine (9.7%) and 9 (3.0%) of the Vibrio spp. isolates were identified as V. parahaemolyticus and V. cholerae, respectively. Thirty (10%) of the Aeromonas spp. isolates were detected as A. hydrophila. L. monocytogenes and V. vulnificus was not detected in any of samples. Antibiotic resistance profile of the V. parahaemolyticus, V. cholerae, A. hydrophila and Listeria spp. isolates was streptomycin (71.4%), teicoplanin (71.4%), ampicillin (87.5%), teicoplanin (75%); streptomycin (80%), ampicillin (75%), cefixime (75%), penicillin (75%), sulfamethoxazole/trimethoprim (100%), tetracycline (75%); erythromycin (93.8%), vancomycin (81.2%), amoxacillin/clavulanic acid (78.6%), ampicillin (85.7%), cephalothin (92.9%), penicillin G (92.9%); and cephalothin (37.5%-42.9%), erythromycin (37.5%-42.9%), penicillin G (37.5%-42.9%), tetracycline (37.5%-42.9%), respectively. Conclusion: As a result, it is thought that fish and shrimp consumed in Turkey may be contaminated with pathogenic Vibrio and Aeromonas species, and antibiotic-resistant isolates may pose a risk to public health.

Özet

Amaç: Bu çalışmada, Türkiye’de tüketilen yüzgeçli balıklarda ve karideslerde Vibrio spp., Aeromonas spp. ve Listeria spp. varlığının ve elde edilen izolatların antibiyotik direnç profilinin disk difüzyon yöntemi kullanılarak belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Araştırmada, farklı zamanlarda toplanan 300 adet deniz ürünü materyal olarak kullanılmıştır. Klasik kültürel yöntemle izolasyonun ardından, izolatları cins düzeyinde doğrulamak ve patojen türleri tanımlamak için klasik PCR yapılmıştır. Bulgular: Yüzgeçli balık ve karides örnekleri Vibrio spp., Aeromonas spp. ve Listeria spp. ile sırasıyla %19,4 (33/170), %14,7 (25/170), %4,1 (7/170) and %13,8 (18/130), %13,1 (17/130), %6,2 (8/130) oranında kontamine bulunmuştur. Vibrio spp. izolatlarının 29 (%9,7)'u ve 9 (%3,0)’u sırasıyla V. parahaemolyticus ve V. cholerae olarak tanımlanmıştır. Aeromonas spp. izolatlarının 30 (%10)'u A. hydrophila olarak tespit edilmiştir. Örneklerin hiçbirinde L. monocytogenes ve V. vulnificus saptanmamıştır. V. parahaemolyticus, V. cholerae, A. hydrophila ve Listeria spp. izolatlarının antibiyotik direnç profili sırası ile streptomisin (%71,4), teikoplanin (%71,4), ampisilin (%87,5), teikoplanin (%75); streptomisin (%80), ampisilin (%75), sefiksim (%75), penisilin (%75), sulfametoksazol/trimetoprim (%100), tetrasiklin (%75); eritromisin (%93,8), vankomisin (%81,2), amoksasilin/ klavulanik asit (%78,6), ampisilin (%85,7), sefalotin (%92,9), penisilin G (%92,9); ve sefalotin (%37,5-%42,9), eritromisin (%37,5-%42,9), penisilin G (%37,5-%42,9), tetrasiklin (%37,5-%42,9) olarak tespit edilmiştir. Öneri: Sonuç olarak, Türkiye'de tüketilen balık ve karideslerin patojen Vibrio ve Aeromonas türleri ile kontamine olabilecekleri ve antibiyotik dirençli izolatların halk sağlığı açısından risk oluşturabileceği düşünülmektedir.

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Cite This Article

KAHRAMAN, H. A., TELLİ, N., DOĞRUER, Y., TELLİ, A. E., TURKAL, G. (2022). Occurrence and antibiotic susceptibility of Vibrio spp., Aeromonas spp. and Listeria spp. in seafoods. Eurasian Journal of Veterinary Sciences, Cilt: 38 - Sayı: 1, 7–16. https://ajindex.com/articles/509742

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References (9)

  1. Abdollahzadeh E, Ojagh SM, Hosseini H, Irajian G, et al., 2016. Prevalence and molecular characterization of Listeria spp. and Listeria monocytogenes isolated from fish, shrimp, and cooked ready-to-eat (RTE) aquatic products in Iran. LWTFood Sci Technol, 73, 205-211.
  2. Ahmed HA, El Bayomi RM, Hussein MA, Khedr MH, et al., 2018. Molecular characterization, antibiotic resistance pattern and biofilm formation of Vibrio parahaemolyticus and Vibrio cholerae isolated from crustaceans and humans. Int J Food Microbiol, 274, 31-37.
  3. Andrews WH, Hammack TS, 2001. United States Food and Drug Administration (USFDA) Bacteriological Analytical Manual. Silver Spring, Md, USA. Food Sampling and Preparation of Sample Homogenate, Chapter 1. Avşar C, Berber İ, Yıldırım AK, 2016. Isolation and characterization of Vibrio spp. from anchovy and garfish in the Sinop province. Turk Hij Tecr Biyol Derg, 73(2), 121- 130. Azwai SM, Alfallani EA, Abolghait SK, Garbaj AM, et al., 2016. Isolation and molecular identification of Vibrio spp. by sequencing of 16S rDNA from seafood, meat and meat products in Libya. Open Vet J, 6(1), 36-43.
  4. Baker-Austin C, Oliver JD, Alam M, Ali A, et al., 2018. Vibrio spp. infections. Nat Rev Dis Primers, 4(1), 1-19.
  5. Bulduklu P, Özer S, 2007. An investigation of motil aeromonads and mesophilic aerobic bacteria count in rainbow trout (Oncorhynchus mykiss Walbaum, 1792) marketed in Mersin. EgeJFAS, 24(1), 97-102.
  6. Chen S, Ge B, 2010. Development of a toxR-based loopmediated isothermal amplification assay for detecting Vibrio parahaemolyticus. BMC Microbiol, 10, 41. Clinical and Laboratory Standards Institute (CLSI), 2016. Performance Standards for Antimicrobial Susceptibility Testing, CLSI Supplement M1000S. 26th ed, Wayne PA. Cocolin L, Rantsiou K, Iacumin L, Cantoni C, et al., 2002. Direct identification in food samples of Listeria spp. and Listeria monocytogenes by molecular methods. Appl Environ Microbiol, 68(12), 6273-6282.
  7. Deng Y, Xu L, Chen H, Liu S, et al., 2020. Prevalence, virulence genes, and antimicrobial resistance of Vibrio species isolated from diseased marine fish in South China. Sci Rep, 10(1), 1-8.
  8. Doğruer Y, Koç U, 2017. The prevalence of the motile Aeromonas species on the shrimps and squids consumed in Antalya. MJAVL, 7(1), 40‐46. Dutta C, Chowdhury S, Panigrahi AK, Sengupta C, 2016. Study of HACCP and microbial quality of seafood during processing in plants around Kolkata. Asian J Anim Sci, 11(2), 140-147.
  9. Elmahdi S, DaSilva LV, Parveen S, 2