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Journals / Experimed / 2018 / Cilt: 8 - Sayı: 3

ER, SOCS1, p15, E-cadherin and RARB are more Likely to be Members of the Methylator Phenotype of Adult and Childhood AML and Their Methylation is Primarly Regulated by an Overexpression of DNMT 3A

Erişkin ve Çocukluk Cağı AML Oluşumunda ER, SOCS1, p15, E-cadherin ve RARB Promoter Metilasyonu Metilatör Fenotip Üyesi Olmaya Aday genlerdir ve Metilasyondan DNMT 3A Başlıca Sorumlu Enzim Olarak Ön Plana Çıkmaktadır

Cumhur G. EKMEKÇİ
Uğur ÖZBEK
Journal
Experimed
Pages
62–70
Publication Date
2018-12-02
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

We analyzed the methylation statuses of ten genes (DAP-kinase, SOCS1,ER, p15, Ecadherin, RARβ, p16, GSTP1, HIC1 and p73) and correlated them with the expression of DNA methyltransferases (DNMTs).Material and Method: We analyzed the methylation profiles usingmethylation specific PCR and COBRA and the expression of DNMTs(DNMT 1, 3A and 3B) by quantitative RT-PCR in 25 pediatric and 25adult AML samples.Results: The ER, SOCS1, p15, E cadherin, and RARB genes methylated together significantly in the same patients and members ofthe methylator phenotype.Conclusion: Our study demonstrated that the gene DNMT3A wasdramatically upregulated and significantly correlated with themethylator phenotype.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: The hypermethylator phenotype is used as a term forboth acute myeloid leukemia (AML) and other tumors.
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Key Findings & Data:Objectives: The hypermethylator phenotype is used as a term forboth acute myeloid leukemia (AML) and other tumors.
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Conclusion & Impact:We analyzed the methylation statuses of ten genes (DAP-kinase, SOCS1,ER, p15, Ecadherin, RARβ, p16, GSTP1, HIC1 and p73) and correlated them with the expression of DNA methyltransferases (DNMTs).Material and Method: We analyzed the methylation profiles usingmethylation specific PCR and COBRA and the expression of DNMTs(DNMT 1, 3A and 3B) by quantitative RT-PCR in 25 pediatric and 25adult AML samples.Results: The ER, SOCS1, p15, E cadherin, and RARB genes methylated together significantly in the same patients and members ofthe methylator phenotype.Conclusion: Our study demonstrated that the gene DNMT3A wasdramatically upregulated and significantly correlated with themethylator phenotype.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Metilatör fenotipi · MSP · epigenetik

Abstract

Objectives: The hypermethylator phenotype is used as a term forboth acute myeloid leukemia (AML) and other tumors. We analyzed the methylation statuses of ten genes (DAP-kinase, SOCS1,ER, p15, Ecadherin, RARβ, p16, GSTP1, HIC1 and p73) and correlated them with the expression of DNA methyltransferases (DNMTs).Material and Method: We analyzed the methylation profiles usingmethylation specific PCR and COBRA and the expression of DNMTs(DNMT 1, 3A and 3B) by quantitative RT-PCR in 25 pediatric and 25adult AML samples.Results: The ER, SOCS1, p15, E cadherin, and RARB genes methylated together significantly in the same patients and members ofthe methylator phenotype.Conclusion: Our study demonstrated that the gene DNMT3A wasdramatically upregulated and significantly correlated with themethylator phenotype.

Özet

Amaç: Hipermetilatör fenotipi, hem akut miyeloid lösemi (AML)hem de diğer tümörler için bir terim olarak kullanılır. On geninmetilasyon durumlarını analiz ettik (DAP-kinaz, SOCS1, ER, p15,Ecadherin, RARβ, p16, GSTP1, HIC1 ve p73) ve bunları DNA metiltransferazların (DNMT’ler) ifadesiyle korele ettik.Gereç ve Yöntem: 25 pediatrik ve 25 yetişkin AML örneğinde metilasyon spesifik PCR ve COBRA ve DNMT’lerin (DNMT 1, 3A ve 3B)ekspresyonunu kullanarak metilasyon profillerini analiz ettik.Bulgular: ER, SOCS1, p15, E cadherin ve RARB genleri, aynı hastalarda ve metilasyon fenotipinin üyeleri arasında önemli ölçüdebirlikte metillendi.Sonuç: Çalışmamız, DNMT3A geninin dramatik bir şekilde yukarıregüle edildiğini ve metilasyon fenotipi ile anlamlı şekilde koreleolduğunu gösterdi.

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Cite This Article

EKMEKÇİ, C. G., ÖZBEK, U. (2018). ER, SOCS1, p15, E-cadherin and RARB are more Likely to be Members of the Methylator Phenotype of Adult and Childhood AML and Their Methylation is Primarly Regulated by an Overexpression of DNMT 3A. Experimed, Cilt: 8 - Sayı: 3, 62–70. https://ajindex.com/articles/50853

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