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Journals / Experimed / 2026 / Volume: 8 Issue: 3

ER, SOCS1, p15, E-cadherin and RARB are more Likely to be Members of the Methylator Phenotype of Adult and Childhood AML and Their Methylation is Primarly Regulated by an Overexpression of DNMT 3A

Erişkin ve Çocukluk Cağı AML Oluşumunda ER, SOCS1, p15, E-cadherin ve RARB Promoter Metilasyonu Metilatör Fenotip Üyesi Olmaya Aday genlerdir ve Metilasyondan DNMT 3A Başlıca Sorumlu Enzim Olarak Ön Plana Çıkmaktadır

Cumhur G. Ekmekçi(Acıbadem Labgen Genetic Diagnosis Center, İstanbul, Turkey)
Uğur Özbek(Department of Medical Genetics, Acıbadem University, İstanbul, Turkey)
Journal
Experimed
Pages
—
Publication Date
2018-12-02
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Our study demonstrated that the gene DNMT3A was dramatically upregulated and significantly correlated with the methylator phenotype.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: The hypermethylator phenotype is used as a term for both acute myeloid leukemia (AML) and other tumors.
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Key Findings & Data:We analyzed the methylation statuses of ten genes (DAP-kinase, SOCS1, ER, p15, Ecadherin, RARβ, p16, GSTP1, HIC1 and p73) and correlated them with the expression of DNA methyltransferases (DNMTs).
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Conclusion & Impact:Conclusion: Our study demonstrated that the gene DNMT3A was dramatically upregulated and significantly correlated with the methylator phenotype.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:MSP · Methylator phenotype · epigenetics

Abstract

Objectives: The hypermethylator phenotype is used as a term for both acute myeloid leukemia (AML) and other tumors. We analyzed the methylation statuses of ten genes (DAP-kinase, SOCS1, ER, p15, Ecadherin, RARβ, p16, GSTP1, HIC1 and p73) and correlated them with the expression of DNA methyltransferases (DNMTs). Material and Method: We analyzed the methylation profiles using methylation specific PCR and COBRA and the expression of DNMTs (DNMT 1, 3A and 3B) by quantitative RT-PCR in 25 pediatric and 25 adult AML samples. Results: The ER, SOCS1, p15, E cadherin, and RARB genes methylated together significantly in the same patients and members of the methylator phenotype. Conclusion: Our study demonstrated that the gene DNMT3A was dramatically upregulated and significantly correlated with the methylator phenotype.

Özet

Amaç: Hipermetilatör fenotipi, hem akut miyeloid lösemi (AML) hem de diğer tümörler için bir terim olarak kullanılır. On genin metilasyon durumlarını analiz ettik (DAP-kinaz, SOCS1, ER, p15, Ecadherin, RARβ, p16, GSTP1, HIC1 ve p73) ve bunları DNA metil transferazların (DNMT’ler) ifadesiyle korele ettik. Gereç ve Yöntem: 25 pediatrik ve 25 yetişkin AML örneğinde metilasyon spesifik PCR ve COBRA ve DNMT’lerin (DNMT 1, 3A ve 3B) ekspresyonunu kullanarak metilasyon profillerini analiz ettik. Bulgular: ER, SOCS1, p15, E cadherin ve RARB genleri, aynı hastalarda ve metilasyon fenotipinin üyeleri arasında önemli ölçüde birlikte metillendi. Sonuç: Çalışmamız, DNMT3A geninin dramatik bir şekilde yukarı regüle edildiğini ve metilasyon fenotipi ile anlamlı şekilde korele olduğunu gösterdi.

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Cite This Article

Ekmekçi, C. G., Özbek, U., *, C. G. E. (2018). ER, SOCS1, p15, E-cadherin and RARB are more Likely to be Members of the Methylator Phenotype of Adult and Childhood AML and Their Methylation is Primarly Regulated by an Overexpression of DNMT 3A. Experimed, Volume: 8 Issue: 3. https://ajindex.com/articles/1116699

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