Journals / Etlik Veteriner Mikrobiyoloji Dergisi / 2006 / Cilt: 17 - Sayı: 1-2

"lnvestigation of antibodies against Chlamydophila abortus (Chlamydia psittaci serotip tip 1) Using Microcomplement Fixation Test (mCFT) and Enzyme-Linked Immunosorbeni Assay (ELISA)

Chlamydophıla Abortus'a (Chlamydıa Psıttacı Serotype 1) Karşı Oluşan Antikorların Mikrokomplement Fikzasyon (mCFT) Ve Enzyme-Lınked Immunosorbent Assay (Elısa) İle Araştırılması

Pages
7–12
Publication Date
2022-10-17
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The seropositivity rates were 30 % in Kahramanmaraş, 27 % in Hatay, 23.5 % in Mersin, 20 % in Kilis, 16.8 % in Gaziantep, 16.7 % in Şanlıurfa, 9.5% in Osmaniye,% 5.0 in Adıyaman, and 2.5% in Adana.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, fıve hundred and fifty serum samples collected from aborted sheep sera from the nine cities (Mersin, Adana, Gaziantep, Adıyaman, Kahramanmaraş, Şanlıurfa, Hatay, Kilis, and Osmaniye) in 2003-2004 lambing season were tested for antibodies against Chlamydophila aborttıs by indirect enzyme-linked immunosorbent assay (ELISA), and microcomplement fıxation test (mCFT).
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Key Findings & Data:In addition to aborted sheep sera, 50 sheep sera from a flock having no abort history were used as control.
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Conclusion & Impact:The seropositivity rates were 30 % in Kahramanmaraş, 27 % in Hatay, 23.5 % in Mersin, 20 % in Kilis, 16.8 % in Gaziantep, 16.7 % in Şanlıurfa, 9.5% in Osmaniye,% 5.0 in Adıyaman, and 2.5% in Adana.
🔬Design:Research Article
📍Scope:2003-2004 Verileri
⚙️Variables:Domain Parameters

Abstract

In this study, fıve hundred and fifty serum samples collected from aborted sheep sera from the nine cities (Mersin, Adana, Gaziantep, Adıyaman, Kahramanmaraş, Şanlıurfa, Hatay, Kilis, and Osmaniye) in 2003-2004 lambing season were tested for antibodies against Chlamydophila aborttıs by indirect enzyme-linked immunosorbent assay (ELISA), and microcomplement fıxation test (mCFT). In addition to aborted sheep sera, 50 sheep sera from a flock having no abort history were used as control. One h undred and seven (19 .5 %) and 161 (29 .3 %) of the 550 serum samples tes te d w ere detected to be positive with ELISA and mCFT, respectively. Although no seropositivity was detected by ELISA, 8 (16 %) sheep sera were positive by mCFT in healthy sheep. The seropositivity rates were 30 % in Kahramanmaraş, 27 % in Hatay, 23.5 % in Mersin, 20 % in Kilis, 16.8 % in Gaziantep, 16.7 % in Şanlıurfa, 9.5% in Osmaniye,% 5.0 in Adıyaman, and 2.5% in Adana.

Özet

Bu çalışmada, Mersin, Adana, Gaziantep, Adıyaman, Kahramanmaraş, Şanlıurfa , Hatay, Kilis ve Osmaniye illerinden 2003 ve 2004 kuzulama sezonunda toplanan 550 yavru atmış koyun kan serumu Chlamydophila abortus'a karşı oluşan antikorlar yönünden indirekt enzyme-linked immunosorbent assay (ELISA) ve mikrokampiement fıkzasyon (mCFT) ile incelendi. Abort yapmış koyun kan serumIanna ilave olarak, 50 abort yapmamış koyundan alınan kan serumu negatif kontrol olarak kullanıldı. İncelenen serum örneklerinin 107'sinde (% 19.5) ELISA, 161'inde (% 29.3) mCFT ile pozitiflik saptandı. Sağlıklı koyunlarda ELISA ile pozitiflik saptanamazken, mCFT ile 8 (% 16.0) serum örneği pozitif bulundu. Serapozitiflik oranları Kahramamnaraş'da % 30, Hatay'da % 27, Mersin'de % 23 .5, Kilis'te % 20, Gaziantep % 16.8, Şanlıurfa'da % 16.7, Osmaniye'de % 9.5, Adıyaman'da % 5.0 ve Adana'da % 2.5 olarak belirlendi.

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Cite This Article

TUNCA, Ş. T., MİRİOĞLU, M., ÇAYA, H., İYİSAN, A. S., ASLANTAŞ, Ö. (2022). "lnvestigation of antibodies against Chlamydophila abortus (Chlamydia psittaci serotip tip 1) Using Microcomplement Fixation Test (mCFT) and Enzyme-Linked Immunosorbeni Assay (ELISA). Etlik Veteriner Mikrobiyoloji Dergisi, Cilt: 17 - Sayı: 1-2, 7–12. https://ajindex.com/articles/506737

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