Journals / Etlik Veteriner Mikrobiyoloji Dergisi / 2015 / Cilt: 26 - Sayı: 1

Antibiotic susceptibilities and isolation of Pseudomonas aeruginosa from clinical mastitis in cattle

Sığırların klinik mastitislerinden Pseudomonas aeruginosa'nın izolasyonu ve antibiyotiklere duyarlılıkları

Pages
16–20
Publication Date
2022-10-17
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a conclusion, usage of danofloxacin, lincomycine/neomycin, cefoperazone and colistin sulphate were proved to bring success in treatment.

📌 3 Key Takeaways

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Objective & Hypothesis:The aims of this study were isolation of environmental mastitis agent -Pseudomonas aeruginosa- from clinical mastitic cattles in Aydin and determination of antibiotic susceptibilities of isolates.
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Key Findings & Data:Totally 200 clinical mastitic milk samples (CMT +3) collected from different centers have been analyzed.
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Conclusion & Impact:As a conclusion, usage of danofloxacin, lincomycine/neomycin, cefoperazone and colistin sulphate were proved to bring success in treatment.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aims of this study were isolation of environmental mastitis agent -Pseudomonas aeruginosa- from clinical mastitic cattles in Aydin and determination of antibiotic susceptibilities of isolates. Totally 200 clinical mastitic milk samples (CMT +3) collected from different centers have been analyzed. Collected samples were brought to The Routine Diagnostic Laboratory of Microbiology Department, Veterinary Faculty, Adnan Menderes University under cold chain. The samples were allowed to incubate for 24 h at 37ºC. For differentiation of Pseudomonas species, selective media were used and isolated bacteria were identified based on biochemical assays. Seven P. aeruginosa strains (3,5%) were isolated and identified from 200 analyzed mastitic milk samples. All the studied strains were found as sensitive to danofloxacin, lincomycine/neomycin, cefoperazone, colistin sulphate (100 %) and resistant to penicillin/novobiocin, amoxycillin clavulanic acide, ceftiofur, kanamycin/cephalexin, erythromycin (100%). Resistant two strains, moderately sensitive 3 strains and sensitive two strains were being present against florfenicol. Five strains were found moderately sensitive (71.0%) to sulfamethoxazole-trimethoprim, while two strains were resistant to same antibiotic. Resistance to oxytetracycline occurred in 5 strains (%71), as two strains were moderately sensitive (29.0%). In this study, isolation and identification of P. aeruginosa from environmental mastitis cases were performed in Aydin province. As a conclusion, usage of danofloxacin, lincomycine/neomycin, cefoperazone and colistin sulphate were proved to bring success in treatment.

Özet

Bu çalışmada Aydın ilinde görülen sığır klinik mastitislerinden çevresel mastitis etkeni olan Pseudomonas aeruginosanın izolasyonu ve izole edilen etkenlerin antibiyotiklere duyarlılıklarının saptanması amaçlanmıştır. Çalışmada Aydın ili ve çevresinde bulunan çeşitli odaklardan toplam 200 adet klinik mastitisli (CMT +3) süt örneği incelenmiş- tir. Alınan örnekler soğuk zincir altında Adnan Menderes Üniversitesi Veteriner Fakültesi Mikrobiyoloji Rutin Teşhis Laboratuvarına getirilmiş ve ekimleri yapılmıştır. Daha sonra 37ºCde 24 saat inkubasyona bırakılmıştır. Pseudomonas türlerinin ayrımında etkene yönelik selektif besiyerleri kullanılmış ve izole edilen bakterilerin biyokimyasal yöntemlere göre identifikasyonu yapılmıştır. Araştırmada kullanılan toplam 200 mastitisli süt örneğinden 7 (%3.5) adet P. aeruginosa suşu izole ve identifiye edilmiştir. İzole ve identifiye edilen P. aeruginosa suşlarına yapılan antibiyotik duyarlılık testleri sonucunda 7 adet suşun tamamı danofloksasin, linkomisin/neomisin, sefaperazon ve kolistin sülfata duyarlı (%100), penisilin/novobiosin, amoksisilin klovulanik asit, kanamisin/sefaleksin ve eritromisine ise dirençli (%100) bulunmuştur. Florfenikole 2 suş dirençli (%29), 3 suş orta düzeyde duyarlı (%42.0) ve 2 suş ise duyarlı (%29.0), sülfamethaksasol-trimethoprime 5 suş orta düzeyde duyarlı (%71.0), 2 suş dirençli (%29.0) ve Oksitetrasiklin açısından ise 2 suş orta düzey duyarlı (%29.0) 5 suş dirençli (%71.0) olarak tespit edilmiştir. Sonuç olarak Aydın ilinde görülen çevresel mastitis vakalarında P. aeruginosanın izolasyonu ve identifikasyonları yapılmış ve danofloksasin, linkomisin/ neomisin, sefaperazon ve kolistin sülfat kullanımının sağaltımda başarı getireceği ortaya konmuştur.

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Cite This Article

ERBAŞ, G., ŞAHİN, C. (2022). Antibiotic susceptibilities and isolation of Pseudomonas aeruginosa from clinical mastitis in cattle. Etlik Veteriner Mikrobiyoloji Dergisi, Cilt: 26 - Sayı: 1, 16–20. https://ajindex.com/articles/506672

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