SavedAdd your journal

Journals / Erciyes Üniversitesi Veteriner Fakültesi Dergisi / 2005 / Cilt: 2 - Sayı: 2

Detection of antibodies in poultry produced against Bordetella avium by microagglutination (MAT) indirect fluorescence antibody (IFA) and ELISA (Enzyme Linked Immunosorbent Assay) techniques

Kanatlı hayvanlarda Bordetella avium'a karşı oluşan antikorların mikroaglutinasyon (MAT) indirekt fluoresan antikor (IFA) ve ELISA (Enzyme Linked Immunosorbent Assay) teknikleri ile saptanması

Fulya OCAK
Pages
85–89
Publication Date
2022-10-18
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was found that 27.1%, 21.0% and 7.2% of turkey sera samples were seropositive with ELISA, MAT and IFAT techniques, respectively, whereas, 14.2%, 9.6% and 17.3% of broiler sera samples were seropositive with ELISA, MAT and IFAT techniques, respectively.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study B.av/iv/rrinfections were detected in broiler and turkey with ELISA, MAT and IFAT techniques.
📊
Key Findings & Data:For this aim, 1050 turkey sera samples collected from Bolu, Izmir, Bigadiç regions and 500 broiler sera samples collected from Ankara region were analysed.
💡
Conclusion & Impact:It was found that 27.1%, 21.0% and 7.2% of turkey sera samples were seropositive with ELISA, MAT and IFAT techniques, respectively, whereas, 14.2%, 9.6% and 17.3% of broiler sera samples were seropositive with ELISA, MAT and IFAT techniques, respectively.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study B.av/iv/rrinfections were detected in broiler and turkey with ELISA, MAT and IFAT techniques. For this aim, 1050 turkey sera samples collected from Bolu, Izmir, Bigadiç regions and 500 broiler sera samples collected from Ankara region were analysed. It was found that 27.1%, 21.0% and 7.2% of turkey sera samples were seropositive with ELISA, MAT and IFAT techniques, respectively, whereas, 14.2%, 9.6% and 17.3% of broiler sera samples were seropositive with ELISA, MAT and IFAT techniques, respectively. Consequently, more seropositive results are obtained with ELISA technique, However, IFAT and MAT techniques can be used for the indirect diagnose of 0. avium infections.

Özet

Bu çalışmada, hindi ve broylerlerde B. avium infeksiyonunun varlığı ELISA, MAT ve IFAT teknikleriyle araştırıldı. Bu amaçla Bolu, İzmir, Bigadiç yörelerinden 1050 hindi ve Ankara çevresinden 500 broyler serum örneği incelendi. Çalışma sonucu hindi serum örneklerinin ELISA ile %27.1, MAT ile %21.0, IFAT ile %17.3'ü pozitif, broyler serum ör¬neklerinin ise ELISA ile %14,2, MAT ile %9.6, IFAT ile %7.2'si pozitif bulunmuştur. Sonuç olarak bu araştırmada ELISA ile B. avium'a karşı daha fazla sayıda seropozitiflik saptandı. Bununla birlikte IFAT ve MAT tekniklerinin de hastalığın indirekt tanısında kullanılabileceği gösterildi.

🔖

Cite This Article

OCAK, F. (2022). Detection of antibodies in poultry produced against Bordetella avium by microagglutination (MAT) indirect fluorescence antibody (IFA) and ELISA (Enzyme Linked Immunosorbent Assay) techniques. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 2 - Sayı: 2, 85–89. https://ajindex.com/articles/504092

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (2)

  1. Akeila MA, Saif YM, 1988. Protection of turkey poults from Bordetella avlum infection and disease by pili and bacterins. Avian Dis., 32:641-649. 2.Andral B, Louzis C, Edlinger E, Newman JA, Toquin D,1984. Respiratory Disease (föinotracheitis) in turkeys in brittany France 1981-1982. II Labarotory findings. Avian Dis., 29: 26-42 3.Anon 1985. Turkey rhinotracheitis of unknown aetiology in England and Wales, preliminary report from the British Vet Rec., 117: 653-654. 4.Arp LH, 1986. Adherence of Bordetella avium to turkey tracheal mucosa: Effects of culture conditions. Am J Vet Res., 47: 2618-2620. 5.Arp LH, Cheville NF, 1984. Tracheal lesions in young turkeys infected with Bordetella avium. Am J Vet Res., 45: 2196-2200. 6.Barbour EK, Brinton KM, Tarkelson SD, Johnson JB, Poss PE, 1991. An Enzyme - Linked immunosorbent assay for detection of Bordetella avium infection in turkey flocks: Sensitivity, Spesifity and Reproducibility. Avian Dis., 35: 308-314. 7.Blackall PJ, Farrah 1985.Isolation of Bordetella avium from poultry. Aust Vet J.,62: 370-372. 8.Conover WJ, 1999.
  2. Practical Nonparametric Statistics, Third Edition. New York: John Waley & Sons 9.Euzeby JP, Raffi AM, 1988. Mise en evidence d'Anticorps anti-Borrelia burdgorferi chez le chien Sondage Epidemiologique en Region Midi-Pyrenees. Rev Med Vet, 139: 589-593. 10.Ficken MD, Edwars JF, Lay JC, Tveter DE, 1985. Tracheal mucus transport rate in normal turkeys and in turkeys infected with Bordetella avium (Alkaligenes faecalis). Avian Dis., 30:154-159. 11.Helwig DH, Arp LH, 1990. Identification antigens recognized after experimental inoculations in turkeys. Am J Vet Res., 51:1188-1191. 12.Hopkins BA, Skeeles GE, Houghteen GE, Story JD, 1988. Development of an Enzyme-Linked immunosorbent Assay for Bordetella avium. Avian Dis., 32:353-361. 13.Jackwood MW, Saif YM, 1985. Efficacy of a commercial turkey coryza vaccine (art-vax) in Turkey poults Avian Dis., 29: 1130-1139. 14.Özdamar K, 2001. SPSS ile Bioistatistik Dör düncü baskı, Kaan Kitabevi Ankara. 15.Page RK, Fletcher OJ, Lukert PD, Rimler R, 1978. Rhinotracheitis in Turkey Poults. Avian Dis., 22:529-534. 16. Panigraphy BL, Grumbles RJ, Terry DL, Hall CF, 1981. Bacterial Coryza in Turkeys in Texas. Poult Sci., 60: 107-113. 17.Raffel RT, Register KB, Marks SA, Temple L, 2002. Prevalence of Bordetella avium in selected wild and domesticated bird in the Eastern USA. J Wildlife Dis., 38: 40-46. 18.Rimler RB, 1985. Turkey coryza: Toxin production by Bordetella