Journals / Erciyes Üniversitesi Veteriner Fakültesi Dergisi / 2008 / Cilt: 5 - Sayı: 1

Detection of myophosphorylase deficiency (glycogen storage disease type v) with PCR-RFLP in charolais cattle in Kayseri region and in Turkey Native cattle breeds

Kayseri bölgesinde yetiştirilen şarole ırkı sığırlarda ve Türkiye yerli sığır ırklarında miyofosforilaz eksikliği (glikojen depo hastalığı tip v) allelinin PCR-RFLP ile belirlenmesi

Pages
21–25
Publication Date
2022-10-18
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

There were no positive results indicating the presence of myophosphorylase deficiency allele in Charolais, Charolais crossbreed and Turkish native cattle breeds.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The aim of this study was to investigate whether or not the myophosphorylase deficiency allele exists in Charolais and in Turkish native cattle breeds in Turkey.
📊
Key Findings & Data:Ten Charolais, 15 Charolais-Simmental crossbreds, 20 Native Black, 20 Gray Steppe, 20 South Anatolian Red, 20 East Anatolian Red and 20 Simmental breed for the control group comprised the 125 cattle used in this study.
💡
Conclusion & Impact:There were no positive results indicating the presence of myophosphorylase deficiency allele in Charolais, Charolais crossbreed and Turkish native cattle breeds.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to investigate whether or not the myophosphorylase deficiency allele exists in Charolais and in Turkish native cattle breeds in Turkey. Ten Charolais, 15 Charolais-Simmental crossbreds, 20 Native Black, 20 Gray Steppe, 20 South Anatolian Red, 20 East Anatolian Red and 20 Simmental breed for the control group comprised the 125 cattle used in this study. The PCR products were digested by StyI restriction enzyme. The resulting fragments were analyzed on 3 % NuSieve agarose gel for the presence or absence of a StyI restriction site. There were no positive results indicating the presence of myophosphorylase deficiency allele in Charolais, Charolais crossbreed and Turkish native cattle breeds.

Özet

Bu çalısmanın amacı Türkiye’de yetistirilen saf ve melez Sarole ırkı sıgırlar ile Türkiye yerli sıgır ırklarında miyofosforilaz eksikligi allelinin bulunup bulunmadıgının arastırılmasıdır. Arastırmada, on bas Sarole, 15 bas Sarole- Simmnetal melezi, 20 bas Yerli Kara, 20 bas Boz Irk, 20 bas Güney Anadolu Kırmızısı, 20 bas Dogu Anadolu Kırmızısı ve kontrol grubu olarak da 20 bas Simmental sıgırı toplam 125 sıgır kullanılmıstır. PCR ürünleri StyI restriksiyon enzimi ile kesilmistir. Elde edilen parçacıklarda StyI restriksiyon bölgesinin varlıgı veya yoklugu % 3’lük NuSieve agaroz jel elektroforezi ile bakılmıstır. Incelenen Sarole, Sarole melezi ve yerli sıgırlarda miyofosforilaz eksikligi alleline rastlanılmamıstır.

🔖

Cite This Article

BAYRAM, D., AKYÜZ, B., İŞCAN, K. M., ERTUĞRUL, O. (2022). Detection of myophosphorylase deficiency (glycogen storage disease type v) with PCR-RFLP in charolais cattle in Kayseri region and in Turkey Native cattle breeds. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 5 - Sayı: 1, 21–25. https://ajindex.com/articles/504035

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (17)

  1. Akyüz B, 2004. Türkiye’deki Holstayn sıgırlarında sıgır lökosit baglanma yetmezliginin (bovine leukocyte adhesion deficiency, BLAD) restriksiyon parçacık uzunluk polimorfizmi (restriction fragment length polymorphism, RFLP) ile belirlenmesi. Doktora Tezi. Ankara Üniv Saglık Bil Enst, Zootekni Programı. Ankara
  2. Akyüz B, Ertugrul O, 2006. Detection of bovine leukocyte adhesion deficiency (BLAD) in Turkish native and Holstein cattle. Acta Vet Hung, 54(2): 173–178
  3. Akyüz B, Ertugrul O, 2008. Türkiye’de Holstayn ve yerli sıgırlarda üridin monofosfat senteaz eksikliginin (DUMPS) belirlenmesi. Ankara Üniv Vet Fak Derg, 55: 57-60
  4. Alpan O, 1993. Sıgır Yetistiriciligi ve Besiciligi
  5. Basım. Ankara: Ankara Üniversitesi Veteriner Fakültesi Zootekni Bölümü, s. 205
  6. Anonim, 2005. Erisim (06.10.2005). http://www.tarim.gov.tr/arayuz/9/icerik.asp? efl= buyukbas/buyukbas.htm&curdir=uretim hayvancilikbuyukbas&fl=sunitohumlama/ sunitohumlama.htm
  7. Citek J, Rehout V, Hajkova J, Pavkova J, 2006. Monitoring of the genetic health of cattle in the Czech Republic. Vet Med, 51(6): 333–339
  8. Citek J, Rehout V, Vecerek L, Hajkova J, 2007. Genotyping glycogen storage disease type II and type V in cattle reared in the Czech Republic. J Vet Med A, 54: 257–259
  9. Distl O, 2005. The use of molecular genetics in eliminating of inherited anomalies in cattle Arch Tierz Dummerstorf, 48(3): 209-218
  10. Fésüs L, Zsolnai A, Anton I, Bárány I, Bozó S, 1999. Short communication BLAD genotypes and cow production traits in Hungarian Holsteins. J Anim Breed Genet, 116: 169-174
  11. Fries R, Ruvinsky A, 1999. The Genetics of Cattle, CABI Publishing, Wallingford, Chapter 6, p. 59
  12. Healy PJ, 1996. Testing for undesirable traits in cattle: an Australian perspective. J Anim Sci, 74:917–922
  13. Jánosa Á, Baranyai B, Dohy J, 1999. Comparison of milk production of the progeny of BLAD-carrier and healthy Holstein bulls in Hungary. Acta Vet Hung, 47(3): 283-289
  14. Johnstone AC, McSporran KD, Kenny JE, Anderson IL, Macpherson GR, Jolly RD, 2004. Myophosphorylase deficiency (glycogen storage disease Type V) in a herd of Charolais cattle in New Zealand: confirmation by PCR-RFLP testing. N Z Vet J, 52(6): 404–408
  15. Kaneko JJ, Harvey JW, Bruss ML, 1997. Clinical Biochemistry of Domestic Animals. 5th Ed., Academic Press London, p. 46,77,434
  16. Kehrli M, Shuster DE, Ackerman MR 1992. Leukocyte adhesion deficiency among Holstein cattle. Cornel Vet, 82(2): 103-109
  17. Kriegesmann B, J