Journals / Erciyes Üniversitesi Veteriner Fakültesi Dergisi / 2017 / Cilt: 14 - Sayı: 2

Detection of Beta-Laktoglobulin (?-LG)/ SacII Gene Polymorphism in Hair and DamascusGoat Breeds in Turkey

Türkiye'de Yetiştirilen Kıl ve Halep Keçilerinde Beta-Laktoglobulin (?-LG)/ SacII Polimorfizminin Belirlenmesi

Pages
107–112
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Deflection from Hardy-Weinberg equilibrium was tracked in the Hair goat breed (P

📌 3 Key Takeaways

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Objective & Hypothesis:This disquisition was aim to detect the allele structures of beta-lactoglobulin (?-LG) gene in Hair (172) and Halep (Damascus) (97) breeds that have been grown in Turkey.
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Key Findings & Data:A total of 269 goats were analized for the ?-LG-SacII polymorphism by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).
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Conclusion & Impact:Deflection from Hardy-Weinberg equilibrium was tracked in the Hair goat breed (P
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

This disquisition was aim to detect the allele structures of beta-lactoglobulin (?-LG) gene in Hair (172) and Halep (Damascus) (97) breeds that have been grown in Turkey. A total of 269 goats were analized for the ?-LG-SacII polymorphism by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). In the examined breeds, digestion the PCR products with SacII restriction enzyme show two alleles (A and B) and two genotypes (AA and AB). The genotype BB was not located on both of Hair and Halep variety. Genotypic frequencies of allele B were 0.721 and 0.948 for AA and 0.279 and 0.052 for AB in order. Deflection from Hardy-Weinberg equilibrium was tracked in the Hair goat breed (P

Özet

Bu çalışmada Türkiye yerli keçi ırklarından Kıl ve Halep (Damascus) ırklarında beta-laktoglobulin (?-LG) gen polimorfizminin belirlenmesi amaçlanmıştır. Çalışmada toplam 269 baş keçide ?-LG genotipleri SacII enzim kesimi ile belirlenmiştir. Örneklere ait PCR ürünlerinin SacII enzimi ile kesimleri sonucunda A ve B olarak isimlendirilen iki allel belirlenmiştir. İncelenen ırklarda AA genotipinin frekansı Kıl keçilerinde 0.721, Halep keçilerinde ise 0.948 olarak belirlenmiştir. AB genotipinin frekansı ise Kıl keçilerinde 0.279, Halep keçilerinde ise 0.052 olarak belirlenmiştir. İncelenen her iki keçi ırkında da BB genotipine rastlanılmamıştır. Çalışma sonunda Kıl keçilerinde Hardy-Weinberg (H-W) dengesinden sapma gözlenmişken (P

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Cite This Article

ARSLAN, K., ÖZDEMİR, F., İLGAR, E. G., ÇINAR, M. U., AKYÜZ, B. (2022). Detection of Beta-Laktoglobulin (?-LG)/ SacII Gene Polymorphism in Hair and DamascusGoat Breeds in Turkey. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 14 - Sayı: 2, 107–112. https://ajindex.com/articles/503823

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References (13)

  1. Ağaoğlu ÖK, Kul BÇ, Akyüz B, Elmaz Ö, Metin MÖ, Saatci M, Ertuğrul O. Identification of ?-lactoglobulin gene SacII polymorphism in Honamli, Hair and Saanen goat breeds reared in Burdur vicinity. Kafkas Univ Vet Fak Derg 2012; 18 (3): 385-8
  2. Ahmed S, Othman OE. Detection of goat ?- lactoglobulin genotypes using restriction fragment length polymorphism. J of Genetic Eng Biotechnol 2005; 3(1): 31-41
  3. Amigo L, Recio I, Ramos M. Genetic polymorphism of ovine milk proteins: Its influence on technological properties of milk-a review. Int Dairy J 2000(1); 10: 135-49
  4. Pedrosa S, Uzun M, Arranz JJ, GutiérrezGil B, San Primitivo F, Bayón Y. Evidence of three maternal lineages in Near Eastern sheep supporting multiple domestication events. Proc Biol Sci 2005; 272(1577): 2211-7
  5. Bonfatti V, Di Martino G, Cecchinato A, Degano L, Carnier P. Effects of beta-kappacasein (CSN2-CSN3) haplotypes, betalactoglobulin (BLG) genotypes, and detailed protein composition on coagulation properties of individual milk of Simmental cows. J Dairy Sci 2010; 93(1): 3809-17
  6. El-Hanafy AA, El-Saadani MA, Eissa M, Maharem GM, Khalifa ZA. Polymorphism of ?-lactoglobulin gene in Barki and Damascus and their cross bred goats in relation to milk yield. Biotechnol Anim Husb 2010; 26(1-2): 1-12
  7. El Hanafy AAM, Qureshi MI, Sabir J, Mutawakil M, Ahmed MMM, El Ashmaoui H, Ramadan HAMI, Abou-Alsoud M, Sadek MA. Nucleotide sequencing and DNA polymorphism studies of beta-lactoglobulin gene in native Saudi goat breeds in relation to milk yield. Czech J Anim Sci 2015; 60(3): 132-8
  8. Elmacı C, Oner Y, Koyuncu M. Allelic frequency of a SacII RFLP at exon 7 of the ?- lactoglobulin gene in Turkish Hair goat breed. AJAVA 2009; 4(1):130-3
  9. Elmacı C, Öner Y, Koyuncu M. Saanen keçilerinde ?-laktoglobulin genotiplerinin PCR-RFLP yöntemi ile belirlenmesi. Hayvansal Üretim Dergisi 2008; 4(1): 1-4
  10. Ertuğrul O, Akyüz B. Halk elinde yetiştirilen Ankara keçilerinde (Capra hircus) bazı kan protein polimorfizmi. Ankara Üniv Vet Fak Derg 2000; 47(1): 23-9
  11. Food and Agriculture Organization (FAO). http://faostat3.fao.org/download/Q/QA/E, Erişim tarihi: 15.05.2015
  12. Guney O, Torun O, Ozuyanık O, Darcan N. Milk production, reproductive and growth performances of Damascus goats under northern Cyprus conditions. Small Rum Res 2006; 65(1-2): 176-9
  13. Gürcan N. Çeşitli Tiftik ve Kıl keçisi popü- lasyonlarında ?-laktoglobulin polimorfizmi. Yüksek lisans tezi, Ankara Ü Fen Bilimleri Enstitüsü, A