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Journals / Erciyes Üniversitesi Veteriner Fakültesi Dergisi / 2022 / Cilt: 19 - Sayı: 2

Commonly Used Normalization Approaches in MicroRNA Expression Profiling

MikroRNA Ekspresyon Profillemesinde Yaygın Kullanılan Normalizasyon Yaklaşımları

Ali Osman TURGUT
Özgecan KORKMAZ AĞAOĞLU
Pages
152–159
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In this review, information about the most commonly used normalization approaches in miRNA expression studies is summarized.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:RT-qPCR, microarray platforms and miRNA sequencing are the most common techniques used to determine microRNA (miRNA) expressions.
📊
Key Findings & Data:One of the most important issues in studies these techniques are used is the normalization of the data by using appropriate normalization method.
💡
Conclusion & Impact:In this review, information about the most commonly used normalization approaches in miRNA expression studies is summarized.
🔬Design:Review & Systematic Survey
📍Scope:Comprehensive Sample
⚙️Variables:Mikroarray · mikroRNA · miRNA dizileme

Abstract

RT-qPCR, microarray platforms and miRNA sequencing are the most common techniques used to determine microRNA (miRNA) expressions. One of the most important issues in studies these techniques are used is the normalization of the data by using appropriate normalization method. The purpose of normalization is to eliminate the effects of biological and technical variations on study results. Numerous normalization approaches are used for normalization of data obtained from different techniques in miRNA expression studies. In this review, information about the most commonly used normalization approaches in miRNA expression studies is summarized.

Özet

MikroRNA (miRNA) ekspresyonlarının belirlenmesinde RT-qPCR, mikroarray platformları ve miRNA dizileme en yaygın kullanılan tekniklerdir. Tüm bu tekniklerin kullanıldığı çalışmalarda en önemli hususlardan biri verilerin uygun normalizasyon yöntemi ile normalize edilmesidir. Normalizasyon ile biyolojik ve teknik varyasyonların sonuçlar üzerine olan etkisinin elimine edilmesi amaçlanmaktadır. MiRNA ekspresyonu çalışmalarında, farklı tekniklerden elde edilen verilerin normalizasyonunda kullanılan çok sayıda normalizasyon yaklaşımı kullanılmaktadır. Bu derlemede, miRNA ekspresyonu çalışmalarında en yaygın kullanılan normalizasyon yaklaşımları hakkında bilgiler özetlenmiştir.

🔖

Cite This Article

TURGUT, A. O., AĞAOĞLU, Ö. K. (2022). Commonly Used Normalization Approaches in MicroRNA Expression Profiling. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 19 - Sayı: 2, 152–159. https://ajindex.com/articles/503631

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