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Journals / Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.) / 2008 / Cilt: 19 - Sayı: 2

Prostaglandin E2 ve hyaluronik asit sıçan dizinde oluşturulan osteokondral defektlerin onarımında etkilidir

Prostaglandin E2 and hyaluronic acid facilitates treatment of osteochondral deffects

Feza KORKUSUZ
Petek KORKUSUZ
Cem Nuri AKTEKİN
Abdullah Yalçın TABAK
Barış BİRİNCİ
Akif Muhtar ÖZTÜRK
Pages
78–83
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These properties of PGE2 and hyaluronic acid will have a role during the development of new strategies in osteochondral defect treatment.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: Restoration of the articular hyaline cartilage is the basic prerequisite to preserve normal function of the synovial joint after injury.
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Key Findings & Data:We have assumed combining hyaluronic acid with prostaglandin E2 (PGE2) will enhance hyaline cartilage formation by stimulating mesenchymal stem cells on fibrin clot scaffold.
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Conclusion & Impact:These properties of PGE2 and hyaluronic acid will have a role during the development of new strategies in osteochondral defect treatment.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: Restoration of the articular hyaline cartilage is the basic prerequisite to preserve normal function of the synovial joint after injury. We have assumed combining hyaluronic acid with prostaglandin E2 (PGE2) will enhance hyaline cartilage formation by stimulating mesenchymal stem cells on fibrin clot scaffold. Material and methods: Rat knee joint osteochondral defects were treated by (a) bone marrow on fibrin clot, (b) hyaluronic acid alone, © bone marrow on fibrin clot combined with PGE2, (d) bone marrow on fibrin clot combined with hyaluronic acid, (e) bone marrow on fibrin clot with hyaluronic acid and PGE2, and (f) a control group left without treatment.Results: Evaluations were carried out at week six in all groups by radiology and histology. Coverage of defective area with various grades of fibrous or hyaline cartilage was noticed in all groups. All treatment groups presented better healing patterns than the control group. Hyaline cartilage with normal matrix staining pattern was observed on Safranin-O stained sections from (1) bone marrow on fibrin clot with PGE2 and (2) bone marrow on fibrin clot with hyaluronic acid and PGE2. Healing response at these groups was statistically better then the control group.Conclusion: This study demonstrates beneficial effects of PGE2 and hyaluronic acid during chondrocyte differentiation. These properties of PGE2 and hyaluronic acid will have a role during the development of new strategies in osteochondral defect treatment.

Özet

korunabilmesi açısından gereklidir. Bu çalışmada hyaluronik asit ve Prostaglandin E2’ nin (PGE2) birlikte kullanılmasının fibrin pıhtı içerine emdirilmiş kemik iliği hücrelerinden hyaline kıkırdak dokusunun gelişmesini uyarabileceği varsayımını araştırdık. Gereç ve yöntemler: Sıçan dizlerinde oluşturulan osteokondral lezyonlar belirlen çalışma protokollerine uygun olarak tedavi edildi. Buna göre gruplar; (1) kemik iliği ve fibrin pıhtı karışımı, (2) sadece hyaluronik asit, (3) fibrin pıhtı, kemik iliği ve PGE2, (4) fibrin pıhtı, kemik iliği ve hyaluronik asit (5) fibrin pıhtı, kemik iliği karışımı ile hyaluronik asit PGE2 karışımı, (6) tedavi verilmeyen kontrol grubu olarak belirlendi. Altıncı haftanın sonunda çalışma sonuçları histolojik olarak değerlendirildi.Sonuçlar: Tüm tedavi gruplarında kontrol grubundan belirgin olarak farklı derecelerde fibröz ve hyaline kıkırdak iyileşmesi saptandı. Fibrin pıhtı, kemik iliği ve hyaluronik asit karışımın kullanıldığı grup ile fibrin pıhtı, kemik iliği karışımı ile hyaluronik asit ve PGE2’nin birlikte kullanıldığı gruplarda Safranin-O ile boyanmış kesitlerde normal hyalin kıkırdak ağ yapısı ile aynı boyama özellikleri gösteren kıkırdak matriksin oluştuğu görüldü. Bu bulgu konrol grubundan anlamlı (p

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Cite This Article

KORKUSUZ, F., KORKUSUZ, P., AKTEKİN, C. N., TABAK, A. Y., BİRİNCİ, B., ÖZTÜRK, A. M. (2022). Prostaglandin E2 ve hyaluronik asit sıçan dizinde oluşturulan osteokondral defektlerin onarımında etkilidir. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.), Cilt: 19 - Sayı: 2, 78–83. https://ajindex.com/articles/498787

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