Journals / Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.) / 2008 / Cilt: 19 - Sayı: 2

Interaction of MC3T3-E1 cells with titanium implants

MC3T3-E1 hücrelerinin titanyum implantlarla etkileşimi

Pages
84–90
Publication Date
2022-10-18
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

In contrast, mineralized nodules were numerous on sand-blasted and sodium titanatecoated surfaces when compared to the polished ones on day 30.Conclusion: This study demonstrated that sand-blasting and sodium titanate coating provided by NaOH favored the attachment, mineralization and early differentiation of osteoblasts on titanium alloys.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: The aim of the present study was to evaluate in-vitro MC3T3- E1 preosteoblastic cell osseointegration on surfaces of polished, sand-blasted (smooth and rough) and sodium titanate coated titanium alloys.
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Key Findings & Data:Materials and methods: MC3T3-E1 cell proliferation and mineralization was assessed comparatively on polished, sand-blasted (smooth and rough) and sodium titanate coated titanium alloys.
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Conclusion & Impact:In contrast, mineralized nodules were numerous on sand-blasted and sodium titanatecoated surfaces when compared to the polished ones on day 30.Conclusion: This study demonstrated that sand-blasting and sodium titanate coating provided by NaOH favored the attachment, mineralization and early differentiation of osteoblasts on titanium alloys.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: The aim of the present study was to evaluate in-vitro MC3T3- E1 preosteoblastic cell osseointegration on surfaces of polished, sand-blasted (smooth and rough) and sodium titanate coated titanium alloys. Materials and methods: MC3T3-E1 cell proliferation and mineralization was assessed comparatively on polished, sand-blasted (smooth and rough) and sodium titanate coated titanium alloys. Cell morphology, attachment and proliferation were also comparatively evaluated using confocal (CM) and scanning electron microscopy (SEM).Results: All implants used in this study were biocompatible. Cells started to attach on the surfaces of the implants following exposure to the in vitro medium for 3 days. The cells were viable and metabolically active as observed by CM. Cell population increased exponentially from day 3 to day 22. Proliferation rate was highest on polished surfaces and lowest on sodium titanate-coated surfaces. In contrast, mineralized nodules were numerous on sand-blasted and sodium titanatecoated surfaces when compared to the polished ones on day 30.Conclusion: This study demonstrated that sand-blasting and sodium titanate coating provided by NaOH favored the attachment, mineralization and early differentiation of osteoblasts on titanium alloys.

Özet

Amaç: Çalışmanın amacı in vitro koşullarda MC3T3-E1 osteoblast öncülü hücre serisinin kemiğe integrasyonunu parlatılmış, ince veya kalın kumlama yapılmış ya da sodium titanat ile kaplanmış titanium implant yüzeylerinde karşılaştırmalı olarak değerlendirmektir. Gereç ve yöntemler: Parlak, ince, kalın kumlama yapılmış ve sodium titanatla yüzey kaplaması uygulanmış titanium implantlar üzerine uygulanan MC3T3-E1 osteoblast öncülü hücrelerde karşılaştırmalı olarak yüzeye tutunma, çoğalma ve mineralizasyon hızları saptandı. Hücre morfolofisi, yapışma ve canlılık taramalı elektron mikroskobu ve konfokal mikroskop ile değerlendirildi.Bulgular: Bu çalışmada kullanılan tüm implantlar doku ile uyumludur. Hücreler titanium yüzeylere deneyin 3. gününden itibaren tutundu. Bu hücrelerin konfokal mikroskopta canlı ve metabolik olarak aktif davranışa sahip oldukları gözlendi. Hücre sayısı 3. günden 22. güne belirgin olarak arttı. Çoğalma hızı parlak yüzeylerde en yüksek, sodium titanat kaplı olanlarda en düşük olarak saptandı. Diğer yandan 30. günde mineralize nodüller ince ve kalın kumlama yapılmış ve sodium titanat kaplı yüzeylerde daha çok sayıda izlendi. Çıkarım: Bu çalışma ince ve kalın kumlama ile sodium titanat yüzey kaplamasının osteoblastların titanium yüzeyine tutunma, mineralizasyon ve erken farklanmalarını uyardığını göstermektedir.

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Cite This Article

KORKUSUZ, F., KORKUSUZ, P., KOÇ, N., ÖZTÜRK, A., ÖNDER, E., TİMUÇİN, M., PURALI, N., GÖRÜR, İ., NOHUTÇU, R., HAKKI, S. S. (2022). Interaction of MC3T3-E1 cells with titanium implants. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.), Cilt: 19 - Sayı: 2, 84–90. https://ajindex.com/articles/498769

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