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Journals / Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.) / 2014 / Cilt: 25 - Sayı: 2

Hiyalüronik asit bupivakainin apoptotik etkisini azaltır mı?

Does hyaluronic acid decrease the apoptotic effect of bupivacaine?

Akın YILMAZ
Akif Muhtar ÖZTÜRK
Mehmet Ali ERGÜN
İrfan GÜNGÖR
Kadir KAYA
Pages
102–106
Publication Date
2022-10-18
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Our study results show that hyaluronic acid against chondrotoxicity of bupivacaine may have a protective effect in a time and dose-dependent manner

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: In this study, we aimed to study the anti- apoptotic effects of hyaluronic acid on the apoptotic effects of bupivacaine in cultured rat chondrocytes in a time and dose- dependent manner.
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Key Findings & Data:Material and methods: The rat chondrocytes were treated with 7.69 ?M, 76.9 ?M, and 384.5 ?M bupivacaine and 50 ?g/ml hyaluronic acid concentrations for six, 24, and 48 hours.
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Conclusion & Impact:Conclusion: Our study results show that hyaluronic acid against chondrotoxicity of bupivacaine may have a protective effect in a time and dose-dependent manner
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: In this study, we aimed to study the anti- apoptotic effects of hyaluronic acid on the apoptotic effects of bupivacaine in cultured rat chondrocytes in a time and dose- dependent manner. Material and methods: The rat chondrocytes were treated with 7.69 ?M, 76.9 ?M, and 384.5 ?M bupivacaine and 50 ?g/ml hyaluronic acid concentrations for six, 24, and 48 hours. At the end of the treatment period, cells were stained with mixture of acridine orange and ethidium bromide. Apoptosis was evaluated using a fluorescence microscope. Results: A significant protective effect of hyaluronic acid on chondrocytes against bupivacaine exposure at 7.69 ?M and 76.9 ?M concentrations, particularly was observed. There was also a significant protective effect in the exposure time at six and 24 hours for 7.69 ?M and 76.9 ?M bupivacaine doses. Conclusion: Our study results show that hyaluronic acid against chondrotoxicity of bupivacaine may have a protective effect in a time and dose-dependent manner

Özet

Amaç: Bu çalışmada, bupivakainin kültüre edilmiş sıçan kondrositleri üzerindeki apoptotik etkisine zaman ve doza bağlı olarak hiyalüronik asitin anti-apoptotik etkisi araştırıldı.Gereç ve yöntemler: Sıçan kondrositleri, 7.69 ?M, 76.9 ?M ve 384.5 ?M bupivakain ile 50 ?g/ml hiyalüronik asit konsantrasyonları ile altı, 24 ve 48 saatlik sürelerde uygulandı. Tedavi süresi sonunda hücreler akridin turuncusu karışımı ve etidium bromid ile boyandı. Apoptoz floresan mikroskobu ile değerlendirildi.Bulgular: Kondrositlerde özellikle 7.69 ?M ve 76.9 ?M konsantrasyonlarında hyalüronik asitin bupivakanin toksik etkisine karşı anlamlı bir koruyucu etki gösterdiği gözlendi. Ayrıca altı ve 24. saatte 7.69 ?M ve 76.9 ?M bupivakain dozlarında maruziyet süresi açısından anlamlı bir koruyucu etki tespit edildi.Sonuç: Çalışma sonuçlarımız, hiyalüronik asidin bupivakainin kondrotoksik özelliğine karşı zaman ve doza bağımlı bir şekilde koruyucu etkisi olabileceğini göstermektedir

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Cite This Article

YILMAZ, A., ÖZTÜRK, A. M., ERGÜN, M. A., GÜNGÖR, İ., KAYA, K. (2022). Hiyalüronik asit bupivakainin apoptotik etkisini azaltır mı?. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.), Cilt: 25 - Sayı: 2, 102–106. https://ajindex.com/articles/498595

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