SavedAdd your journal

Journals / Ege Üniversitesi Ziraat Fakültesi Dergisi / 2022 / Cilt: 59 - Sayı: 3

Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey

Türkiye’nin Iğdır İli’nde sararma ve çalımsı görünüm gösteren biber bitkilerinde ‘Candidatus phytoplasma trifolii’nin varlığı

Abdullah GÜLLER
Mustafa USTA
Gülüstan KORKMAZ
Serap DEMİREL
Zeynelabidin KURT
Pages
429–437
Publication Date
2022-10-17
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

P. trifolii’ in naturally infected peppers in Iğdır province of Turkey was detected using PCR-RFLP and cladistic analysis.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: The objective of this study was to investigate the group/subgroup of phytoplasma agent in peppers showing phytoplasma symptoms.
📊
Key Findings & Data:Material and Methods: In this study, plants collected from Iğdır province in 2020 were analyzed using direct and nested PCR tests, and BLASTn, iphyClassifier, Mega 7, and pDRAW32 programs were used.
💡
Conclusion & Impact:P. trifolii’ in naturally infected peppers in Iğdır province of Turkey was detected using PCR-RFLP and cladistic analysis.
🔬Design:Research Article
📍Scope:2020 Verileri
⚙️Variables:: Clover proliferation group (16SrVI) · Nested PCR · Capsicum annuum

Abstract

Objective: The objective of this study was to investigate the group/subgroup of phytoplasma agent in peppers showing phytoplasma symptoms. Material and Methods: In this study, plants collected from Iğdır province in 2020 were analyzed using direct and nested PCR tests, and BLASTn, iphyClassifier, Mega 7, and pDRAW32 programs were used. Results: In the tests performed, approximately 1.2 kb of DNA fragments specific to phytoplasma were obtained. The 16S rRNA nucleotide sequence (1254 bp in length) (OM663745) revealed that it was showed more than 99.44% nucleotide similarity to other ‘Ca. P. trifolii’ members. The tentative RFLP and phylogenetic analyzes performed proved the ‘Ca. P. trifolii’ the infection from the Clover proliferation group (16SrVI) group and subgroup A in symptomatic pepper plants. Conclusion: The presence of ‘Ca. P. trifolii’ in naturally infected peppers in Iğdır province of Turkey was detected using PCR-RFLP and cladistic analysis.

Özet

Amaç: Bu çalışmanın amacı fitoplazma belirtisi gösteren biberlerde fitoplazma etmeninin grup/alt grubunu araştırmaktır. Materyal ve Yöntem: Bu çalışmada, 2020 yılında Iğdır ilinden toplanan bitkiler direct ve nested PCR testleri kullanılarak analiz edilmiş ve BLASTn, iphyClassifier, Mega 7 ve pDRAW32 programları kullanılmıştır. Araştırma Bulguları: Testlerde, fitoplazmaya spesifik yaklaşık 1.2 kb DNA fragmentleri elde edilmiştir. Belirlenen 16S rRNA nükleotit dizisi (1254 bp) (OM663745) %99.44'ten fazla diğer ‘Ca. P. trifolii’ üyelerine nükleotit benzerliği göstermiştir. Gerçekleştirilen Sanal RFLP ve filogenetik analizler simptom gösteren biber bitkilerinde Clover proliferation group (16SrVI) grubu ve A alt grubundaki ‘Ca. P. trifolii’ etmeninin infeksiyonunu kanıtlamıştır. Sonuç: Türkiye’nin Iğdır ilinde doğal olarak enfekte olmuş biberlerde ‘Ca. P. trifolii’ varlığı PCR-RFLP ve kladistik analiz kullanılarak tespit edilmiştir.

🔖

Cite This Article

GÜLLER, A., USTA, M., KORKMAZ, G., DEMİREL, S., KURT, Z. (2022). Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey. Ege Üniversitesi Ziraat Fakültesi Dergisi, Cilt: 59 - Sayı: 3, 429–437. https://ajindex.com/articles/496438

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (5)

  1. Alp, Ş., M. Usta, H.M. Sipahioğlu & A. Güller, 2016. First report of "Candidatus Phytoplasma solani" on a new host marigold (Tagetes erecta L.). Turkish Journal of Agriculture & Forestry, 40: 311-318. http: //doi.org/10.3906/tar-1506-58
  2. Arocha, Y., G. Plata, J. Franco, G. Maín, S. Veramendi, F. Lazcano, J.L. Crespo, V. Lino, C. Calderόn, R. Llerena, R. Andrew, O. Antezana, A. Gutiérrez, M. Coca & E. Boa, 2009. First report of a 16SrIII Phytoplasma (X-disease group) affecting bell pepper, strawberry (frutilla), Schinus molle and Arracacia xanthorrhiza in Cochabamba, Bolivia. Plant Pathology, 59 (2): 395-395. http: //doi.org/10.1111/j.1365-3059.2009.02235.x Bayram, S., U. Zeybekoglu, G. Soylemezoglu, D. Canik, M. Karavin, A. Cakir & F. Ertunc, 2014. Presence of putative insect vectors of grapevine yellows phytoplasmas in Turkey. Phytopathogenic Mollicutes, 4 (1): 22-26. http: //doi.org/10.5958/2249-4677.2014.00578.7 Bertaccini, A. & I.M. Lee, 2018. “Phytoplasmas: an update,”. in: Phytoplasmas: Plant Pathogenic Bacteria-I, in Characterization and Epidemiology of Phytoplasma-Associated Diseases. Phytoplasmas: Plant Pathogenic Bacteria-I, 1-29. http: //doi.org/10.1007/978-981-13-0119-3_1 Bertaccini, A., 2007. Phytoplasmas: diversity, taxonomy and epidemiology. Frontiers in Bioscience, 12: 673-689. http: //doi.org/10.2741/2092
  3. Beşkeçili, M., N. Güneş & M. Gümüş, 2021. Antalya ili Demre ilçesi biber yetiştiriciliğinde Tomato spotted wilt virus (TSWV) ve Cucumber mosaic virus (CMV) etmenlerinin yaygınlığının belirlenmesi, Ege Universitesi Ziraat Fakültesi Dergisi, 58 (3): 399-405. http: //doi.org/10.20289/zfdergi.799432 Bogoutdinov, D.Z., D. Valyunas, M. Navalinskene & M. Samuitene, 2008. About specific identification of Phytoplasmas in solanaceae crops, Sel'skokhozyaistvennaya Biologiya, 1: 77-80.
  4. Chiykowski, L.N., 1965. A yellows-type virus of alsike clover in Alberta. Canadian Journal of Botany, 43: 527-536. http: //doi.org/10.1139/b65-058 Choueiri, E., P. Salar, F. Jreijiri, S. El Zammar, R. Massaad, H. Abdul‐Nour, J.M. Bové, J.L. Jean-Luc Danet & X. Foissac, 2007. Occurrence and distribution of ‘Candidatus Phytoplasma trifolii’ associated with diseases of solanaceous crops in Lebanon. European Journal of Plant Pathology, 118: 411-416. http: //doi.org/10.1007/s10658-007-9142-8
  5. Erdoğan, O., 2009. Bazı pamuk çeşit adaylarının Verticillium solgunluk hastalığı etmeni (Verticillium dahliae Kleb.)'ne karşı duyarlılıklarının belirlenmesi. Adnan Menderes Üniversitesi