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Journals / Biotech Studies / 2022 / Cilt: 31 - Sayı: 1

Effects of calcium concentration, calcium chelators, calcium channel-blockers on Hsp70a expression in Chlamydomonas reinhardtii

Tuba SEVGİ
Elif DEMİRKAN
Pages
10–16
Publication Date
2022-06-15
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These findings support that both extracellular and intracellular calcium influx is effective in the heat shock response of C. reinhardtii.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, calcium concentration, calcium chelators, and calcium channel blockers that could be effective in triggering the heat shock response in Chlamydomonas reinhardtii were investigated.
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Key Findings & Data:After a short time of heat shock at 40°C, cultures were shifted to 23°C and different concentrations of calcium (0-1 M CaCl2), EGTA (0-50 mM), BAPTA (0-2 mM), lanthanum (0-300 µM), gadolinium (0-350 µM), and verapamil (0-100 µM) applications were performed.
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Conclusion & Impact:These findings support that both extracellular and intracellular calcium influx is effective in the heat shock response of C. reinhardtii.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Arylsulfatase · Heat shock response · Membrane fluidity

Abstract

In this study, calcium concentration, calcium chelators, and calcium channel blockers that could be effective in triggering the heat shock response in Chlamydomonas reinhardtii were investigated. For this purpose, continuously expressed and heat-inducible transformant C. reinhardtii strains were used, and heterologously expressed arylsulfatase activities were detected. After a short time of heat shock at 40°C, cultures were shifted to 23°C and different concentrations of calcium (0-1 M CaCl2), EGTA (0-50 mM), BAPTA (0-2 mM), lanthanum (0-300 µM), gadolinium (0-350 µM), and verapamil (0-100 µM) applications were performed. To compare the arylsulfatase activity results at the transcript level, HSP70A expression level was analyzed. Arylsulfatase activity was increased with the increase of the calcium concentration, in the presence of calcium chelators, blockers, and parallel results were obtained in HSP70A expression level. These findings support that both extracellular and intracellular calcium influx is effective in the heat shock response of C. reinhardtii.

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Cite This Article

SEVGİ, T., DEMİRKAN, E. (2022). Effects of calcium concentration, calcium chelators, calcium channel-blockers on Hsp70a expression in Chlamydomonas reinhardtii. Biotech Studies, Cilt: 31 - Sayı: 1, 10–16. https://ajindex.com/articles/476506

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