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Journals / Avrupa Bilim ve Teknoloji Dergisi / 2020 / Sayı: 19

Filamentous Fungi Associated with the Golot Cheese of the Rize-Ardesen Highlands in Turkey

Türkiye'de Rize-Ardeşen Yaylalarının Golot Peyniri ile İlişkili Filamentli Funguslar

Banu METİN
Pages
435–441
Publication Date
2020-08-31
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Previously unknown sequences of P. solitum PC4 and C. macrocarpum tef1, and a longer version of C. macrocarpum benA were submitted to GenBank.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to identify using molecular methods the filamentous fungi associated with the Golot cheese produced in Ardesen, Rize Province, East Black Sea region, Turkey.
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Key Findings & Data:The other isolates, defined as Penicillium and Cladosporium, could be identified only at the genus level using ITS; therefore, the beta-tubulin (benA) gene and the microsatellite PC4 markers were used to define the remaining Penicillium isolates as P. biforme and P. solitum.
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Conclusion & Impact:Previously unknown sequences of P. solitum PC4 and C. macrocarpum tef1, and a longer version of C. macrocarpum benA were submitted to GenBank.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Golot peyniri · Mikobiyota · Fungal identifikasyon markörleri

Abstract

The aim of this study was to identify using molecular methods the filamentous fungi associated with the Golot cheese produced in Ardesen, Rize Province, East Black Sea region, Turkey. Sixteen fungi were isolated from seven cheese samples and subjected to sequencing analysis using different fungal DNA barcodes. The first marker used, internal transcribed spacer (ITS), allowed identification of Geotrichum candidum and Penicillium roqueforti. The other isolates, defined as Penicillium and Cladosporium, could be identified only at the genus level using ITS; therefore, the beta-tubulin (benA) gene and the microsatellite PC4 markers were used to define the remaining Penicillium isolates as P. biforme and P. solitum. The Cladosporium isolate was identified as C. macrocarpum by analysis using benA and translation elongation factor 1-α (tef1) genes. The most common species associated with Golot cheeses were determined to be P. biforme (56%) and G. candidum (25%). Previously unknown sequences of P. solitum PC4 and C. macrocarpum tef1, and a longer version of C. macrocarpum benA were submitted to GenBank.

Özet

Bu çalışmanın amacı, Doğu Karadeniz Bölgesi, Rize İli, Ardeşen'de üretilen Golot peyniri ile ilişkili filamentli fungusların moleküler yöntemler kullanılarak belirlenmesidir. Yedi peynir örneğinden on altı fungus izole edilmiş ve farklı fungal DNA barkodları kullanılarak dizilim belirleme analizine tabi tutulmuştur. Kullanılan ilk markör olan internal transcribed spacer bölgesi (ITS), Geotrichum candidum ve Penicillium roqueforti'nin identifikasyonuna olanak vermiştir. Diğer izolatlar, ITS kullanılarak sadece cins düzeyinde Penicillium ve Cladosporium olarak tanımlanabilmiştir; bu nedenle, beta-tubulin (benA) geni ve mikrosatellit PC4 markörleri, kalan Penicillium izolatlarını P. biforme ve P. solitum olarak tanımlamak için kullanılmıştır. Cladosporium izolatı, benA ve translation elongation factor 1-α (tef1) genleri kullanılarak analiz edilerek C. macrocarpum olarak tanımlanmıştır. Golot peynirleriyle ilişkili en yaygın türlerin P. biforme (%56) ve G. candidum (%25) olduğu belirlenmiştir. Daha önce bilinmeyen P. solitum PC4 ve C. macrocarpum tef1 dizilimleri ve daha uzun bir C. macrocarpum benA versiyonu GenBank'a sunulmuştur.

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Cite This Article

METİN, B. (2020). Filamentous Fungi Associated with the Golot Cheese of the Rize-Ardesen Highlands in Turkey. Avrupa Bilim ve Teknoloji Dergisi, Sayı: 19, 435–441. https://ajindex.com/articles/471528

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References (10)

  1. Anelli, P., Haidukowski, M., Epifani, F., Cimmarusti, M. T., Moretti, A., Logrieco, A., & Susca, A. (2019). Fungal mycobiota and mycotoxin risk for traditional artisan Italian cave cheese. Food Microbiology, 78, 62-72.
  2. Amrouche, T., Mounier, J., Pawtowski, A., Thomas, F., & Picot, A. (2020). Microbiota associated with dromedary camel milk from Algerian Sahara. Current Microbiology, 77(1), 24-31.
  3. Bachmann, H.P., Bobst, C., Butikofer, U., Casey, M.G., Dalla Torre, M., Frohlich-Wyder, M.T., Furst, M. (2005). Occurrence and significance of Fusarium domesticum alias Anticollanti on smear-ripened cheeses. LWT- Food Science and Technology, 38(4), 399-407.
  4. Balcan, H. (2017).
  5. Personal communication, Rize-Ardesen village. Batra, N., Kaur, H., Mohindra, S., Singh, S., Shamanth, A. S., & Rudramurthy, S. M. (2019). Cladosporium sphaerospermum causing brain abscess, a saprophyte turning pathogen: Case and review of published reports. Journal de Mycologie Medicale, 29(2), 180-184.
  6. Bensch, K., Groenewald, J. Z., Meijer, M., Dijksterhuis, J., Jurjević, Ž., Andersen, B.,. & Samson, R. A. (2018). Cladosporium species in indoor environments. Studies in Mycology, 89, 177-301.
  7. Bensch, K., Braun, U., Groenewald, J. Z., & Crous, P. W. (2012). The genus Cladosporium. Studies in Mycology, 72, 1-401.
  8. Boutrou, R., & Guéguen, M. (2005). Interests in Geotrichum candidum for cheese technology. International journal of food microbiology, 102(1), 1-20.
  9. Caglar, A., Turkoglu, H., Ceylan, Z. G., Dayisoyl, K. S. (1998). Golot peynirinin uretim teknigi ve bilesimi uzerine arastirmalar (Studies on the production technique and composition of Golot cheese). V. Sut ve Sut Urunleri Sempozyumu - Geleneksel Sut Urunleri (No 621, pp. 65-78). Ankara: Milli Produktivite Merkezi Yayinlari. Cakmakci, S. (2011). Turkiye Peynirleri (Cheeses of Turkey). In Hayaloglu A. A. & Ozer B. (eds) Peynir Biliminin Temelleri (pp. 598-600). Izmir: Sidas Medya. Chabalier, C., Ratomahenina, R., Galzy, P., & Dieu, B. (1995). Remarks on the appearance of “violine” on Cantal cheese. Acta Microbiologica et Immunologica Hungarica, 42(2), 199–202.
  10. Costanzo, N., Rodolfi, M., Musarella, R., Ceniti, C., Santoro, A., Britti, D., & Casalinuovo, F. (2018). Microbial quality evaluation of grated cheese samples collected at retail level in Calabria (Italy). Journal of Food Safety, 38(6), e12530. Cuétara, M. S., Alhambra, A., Moragues, M. D., González-Elorza, E., Pontón, J., & del Palacio, A. (2009). Detection of (1→ 3)-β-D-glucan as