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Journals / Bozok Tıp Dergisi / 2018 / Cilt: 8 - Sayı: 4

Investigation on the Therapeutic Effect of the Fulvic Acid upon the Testicular Damage Occurred by the Water Avoidance Stress (WAS)

KRONİK SUDAN KAÇINMA STRESİ İLE TESTİS DOKUSUNDA OLUŞTURULAN HASARI ÜZERİNE FULVİK ASİDİN TEDAVİ EDİCİ ETKİSİNİN ARAŞTIRILMASI

İbrahim SÖĞÜT
Esra ÇİKLER DÜLGER
Canan HÜRDAĞ
Cansu BAHÇIVAN
Pages
60–68
Publication Date
2022-10-11
DOI
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Conclusion: Based on these findings, it has been concluded that fulvic acid may use as a therapeutic agent against testicular damage caused by chronic stress.

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Objective & Hypothesis:Objective: The aim of this study is investigation on the therapeutic effects of a non-toxic agent that has antioxidant, antiapoptotic and anti-inflammatory properties fulvic acid upon the testicular damage occured by water avoidance stress (WAS).
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Key Findings & Data:Material and Methods: 18 Sprague-Dawley male adult rats were distributed into 3 experimental groups: Control (C), Water Avoidance Stress (WAS) and Water Avoidance Stress+Fulvic Acid (WAS+FA).
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Conclusion & Impact:Conclusion: Based on these findings, it has been concluded that fulvic acid may use as a therapeutic agent against testicular damage caused by chronic stress.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The aim of this study is investigation on the therapeutic effects of a non-toxic agent that has antioxidant, antiapoptotic and anti-inflammatory properties fulvic acid upon the testicular damage occured by water avoidance stress (WAS). Material and Methods: 18 Sprague-Dawley male adult rats were distributed into 3 experimental groups: Control (C), Water Avoidance Stress (WAS) and Water Avoidance Stress+Fulvic Acid (WAS+FA). The histological sections obtained from the testes has been stained with the Hematoxylin&Eosin (H&E) and Toluidine Blue (TB) and zonula occuludens-1 (ZO-1), and β-actin and inducible nitric oxide sentase (iNOS) immünohistochemistry labelling has been made to. Total antioksidant status (TAS), total oksidant status (TOS), oxidative stress (OSI), catalase (CAT), glutation peroksidase (GPx) and superoxide dismutase (SOD) levels has been evaluated biochemically. Results: In WAS group it was observed that damage of the seminiferous tubules, increased mast cell activity beneath the tunica albuginea, increased iNOS activitiy and decreased β-aktin and ZO-1 activity in the seminiferous tubules. It was also observed increased TOS and OSI levels and decreased TAS, SOD, CAT and GPx levels in the same group. Healing was observed in the results of KS+FA group with fulvic acid replacement. Conclusion: Based on these findings, it has been concluded that fulvic acid may use as a therapeutic agent against testicular damage caused by chronic stress.

Özet

Amaç: Çalışma, toksik olmayan bir ajan olan; antioksidan, antiapoptotik ve antiinflamatuvar özelliklere sahipfulvik asit (FA)’in kronik sudan kaçınma stresinin (KS) testis dokusunda oluşturduğu hasara karşı tedavi edicietkisinin araştırılmasıdır.Gereç ve Yöntem: Çalışmamızda 18 adet Sprague-Dawley erişkin erkek sıçan 3 eşit gruba ayrıldı: Kontrol(K), Kronik Stres (KS) ve Kronik Stres+Fulvik Asit (KS+FA). Testis dokusundan elde edilen histolojik kesitlerHematoksilen&Eozin (H&E) ve Toluidin mavisi (TM) ile boyandı; zonula okludens-1 (ZO-1), β-aktin veindüklenebilir nitrik oksit sentaz (iNOS) immünohistokimya işaretlemeleri yapıldı. Biyokimyasal olarakdokulardaki total antioksidan seviyesi (TAS), total oksidan seviyesi (TOS), oksidatif stres indeksi (OSİ), katalaz(KAT), glutatyon peroksidaz (GPx) ve süperoksit dismutaz (SOD) düzeyleri ölçüldü.Bulgular: KS grubunda hasarlı seminifer tübüller, tunika albuginea komşuluğunda artmış mast hücre aktivitesi,tübül içerisinde artmış iNOS aktivitesi ve azalmış β-aktin ve ZO-1 aktivitesi gözlendi. Bu grupta ayrıca TOSve OSI düzeylerinin arttığı; TAS, SOD, KAT ve GPx düzeylerinin azaldığı görüldü. KS+FA grubunda, fulvik asitkullanımıyla bu bulgularda iyileşme olduğu gözlendi.Sonuç: Bu bulgulara dayanarak fulvik asitin kronik stresin oluşturduğu testis hasarına karşı tedavi edici bir ajanolarak kullanılabileceği sonucuna varılmıştır.

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Cite This Article

SÖĞÜT, İ., DÜLGER, E. Ç., HÜRDAĞ, C., BAHÇIVAN, C. (2022). Investigation on the Therapeutic Effect of the Fulvic Acid upon the Testicular Damage Occurred by the Water Avoidance Stress (WAS). Bozok Tıp Dergisi, Cilt: 8 - Sayı: 4, 60–68. https://ajindex.com/articles/469028

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