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Journals / Bozok Tıp Dergisi / 2019 / Cilt: 9 - Sayı: 3

Effct of Grape Seed Extract of Silver Nanopartiles on TNF-ɑ and BNP Expression and Histologic Changes in Endotoxic Heart Tissue

GÜMÜŞ NANOPARTİKÜLLERİNİN ÜZÜM ÇEKİRDEĞİ EKSTRAKTININ ENDOTOKSİK KALP DOKUSUNDAKİ HİSTOLOJİK DEĞİŞİKLERE VE TNF-ɑ VE BNP EKSPRESYONUNA ETKİSİ

Züleyha DOĞANYİĞİT
Aslı OKAN
Fatma Öztürk KÜP
Burçin OÇAK
Ali Tuğrul AKİN
Emin KAYMAK
Pages
87–96
Publication Date
2022-10-11
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Tumor necrosis factor-alpha (TNF- α) and BNP immunoreactiity results were signifiantly increased in the LPS group compared to the control group, whereas increased TNF- α and BNP immunoreactiity were found to be similar to the control group in AgNP treated groups with LPS.

📌 3 Key Takeaways

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Objective & Hypothesis:Objecties: Although many strategies have been developed to understand and treat the pathophysiological mechanisms of endotexemia induced by lipopolysaccharide (LPS), it is stil one of the most important problems of intensive care units.
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Key Findings & Data:In this study, we aimed to determine whether there is a protectie effct of biologically synthesized silver nanopartiles (AgNP) on endotoxic shock by using grape seed to reveal the changes in brain natriureti peptie (BNP) expressions and histological changes in heart tisue. 80 Wistar albino adult male rats were divided into 8 equal groups.
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Conclusion & Impact:Tumor necrosis factor-alpha (TNF- α) and BNP immunoreactiity results were signifiantly increased in the LPS group compared to the control group, whereas increased TNF- α and BNP immunoreactiity were found to be similar to the control group in AgNP treated groups with LPS.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objecties: Although many strategies have been developed to understand and treat the pathophysiological mechanisms of endotexemia induced by lipopolysaccharide (LPS), it is stil one of the most important problems of intensive care units. In this study, we aimed to determine whether there is a protectie effct of biologically synthesized silver nanopartiles (AgNP) on endotoxic shock by using grape seed to reveal the changes in brain natriureti peptie (BNP) expressions and histological changes in heart tisue. 80 Wistar albino adult male rats were divided into 8 equal groups. At the end of the study, while a healthy normal heart image was observed in control and AgNP treated groups, cell nuclei were reduced and structurally irregular in the LPS group. In the groups treated with LPS and AgNP, a close to normal image was observed. Tumor necrosis factor-alpha (TNF- α) and BNP immunoreactiity results were signifiantly increased in the LPS group compared to the control group, whereas increased TNF- α and BNP immunoreactiity were found to be similar to the control group in AgNP treated groups with LPS.

Özet

ipopolisakkarit (LPS) tarafıdan oluşturulan endotokseminin patofiyolojik mekanizmalarının anlaşılmasında ve tedavisinde pek çok strateji geliştiilmesine rağmen, hala yoğun bakım ünitelerinin en önemliproblemlerinden biridir. Bu çalışmada, endotoksik şok üzerine üzüm çekirdeği kullanılarak biyolojik olaraksentezlenmiş gümüş nanopartiüllerin (AgNP) koruyucu bir etkisi olup olmadığının kalp dokusundaki histolojik değişiklikler ve beyin natriüreti pepti (BNP) ekspresyonlarındaki değişimler ortaya konularak belirlenmesi amaçlanmıştı. Çalışma için 80 adet Wistar albino cinsi yetikin erkek sıçan 8 eşit gruba ayrılmıştı.Çalışma sonucunda Kontrol ve AgNP uygulanan gruplarda sağlıklı normal bir kalp görüntüsü izlenirken, LPSuygulanan grupta hücre çekirdeklerinde küçülme ve yapısal olarak düzensizlik görülmüştür. LPS ile birlikteAgNP uygulanan gruplarda normale yakın görüntü izlenmişti. Tümör nekroze edici faktör-alfa (TNF-α) veBNP immünreaktiite sonuçlarının LPS grubunda kontrol grubuna göre istatitisel olarak anlamlı bir şekildearttı, LPS ile birlikte AgNP uygulanan gruplarda ise artmış olan TNF-α ve BNP immünreaktiitesinin kontrolgrubuna benzer şekilde olduğu görülmüştür.

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Cite This Article

DOĞANYİĞİT, Z., OKAN, A., KÜP, F. Ö., OÇAK, B., AKİN, A. T., KAYMAK, E. (2022). Effct of Grape Seed Extract of Silver Nanopartiles on TNF-ɑ and BNP Expression and Histologic Changes in Endotoxic Heart Tissue. Bozok Tıp Dergisi, Cilt: 9 - Sayı: 3, 87–96. https://ajindex.com/articles/468611

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