Journals / Trakya University Journal of Natural Sciences / 2017 / Cilt: 18 - Sayı: 2

Determination of Acute Effects of 2,4-Dichlorophenoxyacetic Acid on Antioxidant Enzymes and Lipid Peroxidation Levels in Zebrafish (Danio rerio Hamilton, 1822) Gills

2,4-D (DİKLOROFENOKSİASETİK ASİT)’İN ZEBRA BALIĞI (Danio rerio) SOLUNGAÇLARINDA ANTİOKSİDAN ENZİMLER VE LİPİD PEROKSİDASYON SEVİYESİ ÜZERİNE AKUT ETKİLERİNİN BELİRLENMESİ

Pages
143–148
Publication Date
2017-10-20
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

No significant difference was found in CAT activity compared to the control group.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study is to determine the potential of 2,4-D (2,4-Dichlorophenoxyacetic acid) herbicide in causing acute oxidative stress in the gills of zebrafish (Danio rerio Hamilton, 1822) using spectrophotometric methods.
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Key Findings & Data:Test animals except the control group (Group-K) were exposed to sublethal doses (0.1ppm, 0.5ppm and 1ppm) of 2,4-D herbicide for 96 hours.
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Conclusion & Impact:No significant difference was found in CAT activity compared to the control group.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:antioksidan enzimler · solungaç · zebra balığı

Abstract

The aim of this study is to determine the potential of 2,4-D (2,4-Dichlorophenoxyacetic acid) herbicide in causing acute oxidative stress in the gills of zebrafish (Danio rerio Hamilton, 1822) using spectrophotometric methods. Test animals except the control group (Group-K) were exposed to sublethal doses (0.1ppm, 0.5ppm and 1ppm) of 2,4-D herbicide for 96 hours. Malondialdehyde (MDA) was used to determine lipid peroxidation levels, and reduced glutathione (GSH), catalase enzyme activity (CAT) and total protein (TP) levels were determined to determine their effects on antioxidant systems in gill tissues. In this study, total protein levels in gills of zebrafish exposed to sublethal doses of 2,4-D were observed to be reduced compared to the control group. MDA levels significantly increased compared to the control group. GSH levels increased in the highest dose of 2,4-D herbicide but decreased in other doses. No significant difference was found in CAT activity compared to the control group.

Özet

Bu çalışmanın amacı; 2-4-D (2,4-Diklorofenoksiasetik asit) herbisitinin, zebra balığı (Danio rerio Hamilton, 1822) solungaçlarında akut oksidatif strese neden olma potansiyellerinin spektrofotometrik yöntemlerle belirlenmesidir. Kontrol grubu (Grup-K) dışındaki balıklar, 96 saat süreyle 2-4-D herbisitinin subletal dozlarının (0.1, 0.5 ve 1 ppm) etkisine bırakılmıştır. Solungaç dokusunda lipid peroksidasyon seviyelerinin belirlenmesi amacıyla malondialdehit (MDA), antioksidan sistemlere etkisini belirlemek amacıyla da indirgenmiş glutatyon (GSH), katalaz enzim aktivitesi (CAT) ve total protein (TP) seviyeleri spektrofotometrik yöntemler kullanılarak belirlenmiştir. Bu çalışmada 2,4-D’nin subletal dozlarına maruz bırakılan zebra balıklarının solungaçlarında total protein seviyelerinin kontrol grubuna oranla azaldığı gözlenmiştir. MDA seviyeleri kontrol grubuna oranla önemli ölçüde artmıştır. GSH seviyeleri 2-4-D herbisitinin en yüksek dozunda artarken diğer gruplarda azalmıştır. CAT aktivitesinde ise kontrol grubuna oranla önemli bir fark gözlenmemiştir.

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Cite This Article

KAYHAN, F. E., KAYMAK, G., Akbulut, C., ERTUĞ, N. D. Y. (2017). Determination of Acute Effects of 2,4-Dichlorophenoxyacetic Acid on Antioxidant Enzymes and Lipid Peroxidation Levels in Zebrafish (Danio rerio Hamilton, 1822) Gills. Trakya University Journal of Natural Sciences, Cilt: 18 - Sayı: 2, 143–148. https://ajindex.com/articles/46613

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