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Journals / Balkan Medical Journal / 2018 / Cilt: 35 - Sayı: 1

Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis

Nazan ATALAN
Arzu ERGEN
Leyla ACAR
E Hande KARAGEDİK
Pages
30–35
Publication Date
2022-10-11
DOI
—
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No significantdifferences were found in these 2 polymorphismsbetween the patient and control groups (p>0.05).Conclusion: Our results showed the AA genotype andthe A allele of the tumour necrosis factor-alpha (-308G/A) polymorphism may be used as a predictor ofelevated tumour necrosis factor-alpha levels in patientswith sepsis.

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Objective & Hypothesis:Background: The humoral system is activated andvarious cytokines are released due to infections intissues and traumatic damage.
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Key Findings & Data:Nuclear factor-kappa Bdimers are encoded by nuclear factor-kappa B genesand regulate transcription of several crucial proteins ofinflammation such as tumour necrosis factor-alpha.Aims: To investigate the possible effect ofpolymorphisms on tumour necrosis factor-alpha serumlevels with clinical and prognostic parameters of sepsisby determining the nuclear factor-kappa B-1-94 ins/del ATTG and tumour necrosis factor-alpha (-308 G/A)gene polymorphisms and tumour necrosis factor-alphaserum levels.Study Design: Case-control study.Methods: Seventy-two patients with sepsis and 104healthy controls were included in the study.
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Conclusion & Impact:No significantdifferences were found in these 2 polymorphismsbetween the patient and control groups (p>0.05).Conclusion: Our results showed the AA genotype andthe A allele of the tumour necrosis factor-alpha (-308G/A) polymorphism may be used as a predictor ofelevated tumour necrosis factor-alpha levels in patientswith sepsis.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background: The humoral system is activated andvarious cytokines are released due to infections intissues and traumatic damage. Nuclear factor-kappa Bdimers are encoded by nuclear factor-kappa B genesand regulate transcription of several crucial proteins ofinflammation such as tumour necrosis factor-alpha.Aims: To investigate the possible effect ofpolymorphisms on tumour necrosis factor-alpha serumlevels with clinical and prognostic parameters of sepsisby determining the nuclear factor-kappa B-1-94 ins/del ATTG and tumour necrosis factor-alpha (-308 G/A)gene polymorphisms and tumour necrosis factor-alphaserum levels.Study Design: Case-control study.Methods: Seventy-two patients with sepsis and 104healthy controls were included in the study. In order todetermine the polymorphisms of nuclear factor-kappaB-1-94 ins/del ATTG and tumour necrosis factor-alpha(-308 G/A), polymerase chain reaction–restrictionfragment length polymorphism analysis was performedand serum tumour necrosis factor-alpha levels weredetermined using an enzyme-linked immunosorbentassay.Results: We observed no significant differences intumour necrosis factor-alpha serum levels between thestudy groups. In the patient group, an increase in thetumour necrosis factor-alpha serum levels in patientscarrying the tumour necrosis factor-alpha (-308 G/A) Aallele compared to those without the A allele was foundto be statistically significant. Additionally, an increase inthe tumour necrosis factor-alpha serum levels in patientscarrying tumour necrosis factor-alpha (-308 G/A) AAgenotype compared with patients carrying the AG or GGgenotypes was statistically significant. No significantdifferences were found in these 2 polymorphismsbetween the patient and control groups (p>0.05).Conclusion: Our results showed the AA genotype andthe A allele of the tumour necrosis factor-alpha (-308G/A) polymorphism may be used as a predictor ofelevated tumour necrosis factor-alpha levels in patientswith sepsis.

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Cite This Article

ATALAN, N., ERGEN, A., ACAR, L., KARAGEDİK, E. H. (2022). Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis. Balkan Medical Journal, Cilt: 35 - Sayı: 1, 30–35. https://ajindex.com/articles/461026

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