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Journals / Balkan Medical Journal / 2019 / Cilt: 36 - Sayı: 2

Cytotoxic Effects of Some Flavonoids and Imatinib on the K562 Chronic Myeloid Leukemia Cell Line: Data Analysis Using the Combination Index Method

Hüsniye BİRMAN
Gülay MELİKOĞLU
Serap KURUCA
Ferdane DANIŞMAN KALINDEMİRTAŞ
Eda CANDÖKEN
Sema Bilgiç GAZİOĞLU
Pages
96–105
Publication Date
2022-10-11
DOI
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⚡ TL;DR(Too Long; Didn't Read)

These results were statistically significant (p

📌 3 Key Takeaways

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Objective & Hypothesis:Background: Flavonoids are natural compounds with antioxidant, anticarcinogenic, and anti-inflammatory effects.Aims: To determine the cytotoxic effects of flavonoids and drug resistance related to P-gp on K562 human chronic myeloid leukemia cells.
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Key Findings & Data:We also aimed to evaluate the therapeutic potential of imatinib and flavonoid combinations.Study Design: Cell culture study.Methods: In this study, K562 cells were treated with apigenin, luteolin, 5-desmethyl sinensetin and the anticancer drug imatinib mesylate.
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Conclusion & Impact:These results were statistically significant (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background: Flavonoids are natural compounds with antioxidant, anticarcinogenic, and anti-inflammatory effects.Aims: To determine the cytotoxic effects of flavonoids and drug resistance related to P-gp on K562 human chronic myeloid leukemia cells. We also aimed to evaluate the therapeutic potential of imatinib and flavonoid combinations.Study Design: Cell culture study.Methods: In this study, K562 cells were treated with apigenin, luteolin, 5-desmethyl sinensetin and the anticancer drug imatinib mesylate. The effect of flavonoids on K562 cell proliferation was detected using the 3-(4,5-dimethylthiazolyl)2,5‑diphenyl‑tetrazolium bromide assay. Concentrations of apigenin, luteolin, and 5-desmethyl sinensetin ranging from 25 to 200 μM and of imatinib from 5 to 50 μM administered for 72 h were studied. Apoptosis/necrosis and P-gp activity were measured using flow cytometry. The combined effects of different concentrations of flavonoids with imatinib were evaluated according to combination index values calculated using CompuSyn software.Results: In our study, the IC50 values for apigenin, luteolin, and 5-desmethyl sinensetin were found to be 140 μM, 100 μM, and >200 μM, respectively. Luteolin (100 μM) had the highest cytotoxic activity of these flavonoids. These results were statistically significant (p

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Cite This Article

BİRMAN, H., MELİKOĞLU, G., KURUCA, S., KALINDEMİRTAŞ, F. D., CANDÖKEN, E., GAZİOĞLU, S. B. (2022). Cytotoxic Effects of Some Flavonoids and Imatinib on the K562 Chronic Myeloid Leukemia Cell Line: Data Analysis Using the Combination Index Method. Balkan Medical Journal, Cilt: 36 - Sayı: 2, 96–105. https://ajindex.com/articles/460958

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