Journals / Bahçe / 2020 / Cilt: 49 - Sayı: 1

ANTIFUNGAL ACTIVITY OF Humulus lupulus L. FLOWER EXTRACT AGAINST SOME PLANT PATHOGEN FUNGI

BAZI BİTKİ PATOJENİ FUNGUSLARA KARŞI Humulus lupulus L. BİTKİ EKSTRAKTININ ANTİFUNGAL AKTİVİTESİ

Journal
Bahçe
Pages
113–118
Publication Date
2022-10-10
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

In the results of research; methanol and n-Hexan extracts obtained from H. lupulus flowers in vitro conditions Sclerotinia sclerotiorum and Fusarium oxysporum f. sp. lycopersici on fungicidal activities.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, methanol and n-hexan extracts obtained from flowers of Humulus lupulus L. (Hops) grown in Tokat ecological conditions were investigated by Sclerotinia sclerotiorum (Ss) and Fusarium oxysporum f. sp. lycopersici (Fol) pathogens was investigated antifungal activity against in vitro condition.
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Key Findings & Data:Mycelial Growth (MG), Mycelium Growth Inhibition (MGE) activity levels of 0.1, 0.5, 1 and 2 mg/ml doses of methanol and n-hexan extracts against pathogens were determined and lethal dose (LD₅₀) was calculated.
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Conclusion & Impact:In the results of research; methanol and n-Hexan extracts obtained from H. lupulus flowers in vitro conditions Sclerotinia sclerotiorum and Fusarium oxysporum f. sp. lycopersici on fungicidal activities.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, methanol and n-hexan extracts obtained from flowers of Humulus lupulus L. (Hops) grown in Tokat ecological conditions were investigated by Sclerotinia sclerotiorum (Ss) and Fusarium oxysporum f. sp. lycopersici (Fol) pathogens was investigated antifungal activity against in vitro condition. Agar diffusion method was used for activity studies. Mycelial Growth (MG), Mycelium Growth Inhibition (MGE) activity levels of 0.1, 0.5, 1 and 2 mg/ml doses of methanol and n-hexan extracts against pathogens were determined and lethal dose (LD₅₀) was calculated. Generally on pathogens; n-hexane extract was found to be more effective than methanol extract. For MG; n-hexan extract was effective at a dose of 2 mg/ml against Sc and Fol to 90% and 60% respectively. It was determined that it inhibited Sc and Fol at a dose of 2 mg/ml in methanol extract to 84% and 83%, respectively. LD₅₀ was calculated for Sc for n-hexane and methanol extracts; 0.021 to 0.193 mg/ml, respectively, for Fol; 0.616 to 0.273 mg/ml, respectively. In the results of research; methanol and n-Hexan extracts obtained from H. lupulus flowers in vitro conditions Sclerotinia sclerotiorum and Fusarium oxysporum f. sp. lycopersici on fungicidal activities.

Özet

Bu çalışmada, Tokat ekolojik koşullarda yetişen Humulus lupulus L.(Şerbetçiotu) bitkisinin çiçeklerinden elde edilen methanol ve n-hexan ekstraktlarının Sclerotinia sclerotiorum ve Fusarium oxysporum f. sp. lycopersici (Fol) patojenlerine karşı antifungal aktivitesi in vitro şartlarda araştırılmıştır. Aktivite çalışmalarında, agar diffuzyon yöntemi kullanılmıştır. Methanol ve n-Hexan ekstraktlarının patojenlere karşı, 0.1, 0.5, 1 ve 2 mg/ml dozlarının Miselyum Gelişimi (MG), Miselyum Gelişim Engelleme (MGE) etkinlik düzeyleri belirlenmiş ve letal doz (LD₅₀) hesaplanmıştır. Patojenler üzerine; hexan ekstratı, metanol ekstratına göre daha etkili olduğu bulunmuştur. MG için; Hexan esktraktının 2 mg/ml dozunda Sc ve Fol’e karşı sırasıyla %90 ile %60 oranında etkili olduğu, yine MG için; methanol ekstraktında 2 mg/ml dozunda Sc ve Fol’e karşı sırasıyla %84 ile %83 oranında engellediği belirlenmiştir. LD₅₀ hexan ve methanol ekstratları için Sc için; sırasıyla 0.021 ile 0.193 mg/ml, Fol için; sırasıyla 0.616 ile 0.273 mg/ml olarak hesaplanmıştır. Çalışma sonucunda; H. lupulus çiçeklerinden elde edilen Methanol ve n-Hexan ekstraktlarının in vitro şartlarda Sclerotinia sclerotiorum ve Fusarium oxysporum f. sp. lycopersici üzerine fungusidal aktivitelerinin olduğu belirlenmiştir.

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Cite This Article

BAYAR, Y., YILAR, M., ONARAN, A. (2022). ANTIFUNGAL ACTIVITY OF Humulus lupulus L. FLOWER EXTRACT AGAINST SOME PLANT PATHOGEN FUNGI. Bahçe, Cilt: 49 - Sayı: 1, 113–118. https://ajindex.com/articles/459394

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References (13)

  1. Anonim, 2019. Kocaeli bitkileri. https://kocaelibitkileri.com/humulus-lupulus/ (Erişim Tarihi: 25.10.2019)
  2. Bağcı, İ., 2009. Şerbetçiotunda (Humulus lupulus L.) yapraktan uygulanan gübrenin verim ve kaliteye etkileri (Doktora Tezi). Ankara Üniversitesi Fen Bilimleri Enstitüsü, Tarla Bitkileri Anabilim Dalı, 105s
  3. Bajpai, V., S., Chul, 2009. Antifungal activity of leaf essential oil and extracts of Metasequoia glyptostroboides Miki ex Hu. Journal of the American Oil Chemists Society 87(3):327-336
  4. Barbetti, M., S.K., Banga, T. D., Fu, Y.C.,Li, D., Singh, S.Y., Liu, X.T., Ge, S.S., Banga, 2014. Comparative genotype reactions to Sclerotinia sclerotiorum within breeding populations of Brassica napus and B. juncea from India and China. Euphytica. 197:47-59
  5. Bhattacharya, S., S. Virani, M. Zavro, G.J. Haas, 2003. Inhibition of Streptococcus mutants and other oral streptococci by hop (Humulus lupulus L.) constituents. Econ. Bot., 57:118-125
  6. Bocquet, L., S. Sahpaz, N. Bonneau, C. Beaufay, S. Mahieux, J. Samaillie, V. Roumy, J. Jacquin, S. Bordage, T. Hennebelle, F. Chai, J. Quetin-Leclercq, C. Neut, C. Riviere, 2019. Phenolic compounds from Humulus lupulus as natural antimicrobial products: new weapons in the fight against methicillin resistant Staphylococcus aureus, Leishmania mexicana and Trypanosoma brucei strains. Molecules 24:1-26, 1024
  7. Chadwick, L.R., G.F. Pauli, N.R. Farnsworth, 2006. The pharmacognosy of Humulus lupulus L. (hops) with an emphasis on estrogenic properties. Phytomedicine. 13(1-2):119-131
  8. Dulger, B., N. Hacıoğlu, 2008. Antifungal activity of endemic Salvia tigrina in Turkey. Tropical Journal of Pharmaceutical Research 7(3):1051-1054
  9. Elgorban, A.M., A.H. Bahkali, M.A. El-Metwally, M. Elsheshtawi, M.A. Abdel-Wahab, 2015. In vitro antifungal activity of some plant essential oils. International Journal of Pharmacology 11(1):56-61
  10. Garkoti, A., V. Kumar, H.S. Tripathi, 2013. Management of vascular wilt of lentil through aqueous plant extracts in Tarai region of Uttara hand state. The Bioscan 8(2):473-476
  11. Haas, G.J., R. Barsoumian, 1994. Antimicrobial activity of hop resins. J. Food Prot. 57:59-61
  12. Hassanein, N.M., M.A. Abou Zeid, A. Khayria, K.A. Youssef, D.A. Mahmoud, 2010. Control of tomato early blight and wilt using aqueous extract of neem leaves. Phytopathologia Mediterranea 49(2):143-151
  13. Kadıoğlu, I., Y. Yanar, 2004. Allelopathic effects of plant extracts against seed germination of some weeds