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Journals / Anadolu Kliniği Tıp Bilimleri Dergisi / 2021 / Cilt: 26 - Sayı: 2

Evaluation of Vascular Endothelial Growth Factor, Hypoxia-inducible Factor 1-Alpha, and Bacterial Plaque Composition in Periodontal Health and Disease, with or without Bruxism: A Non-Randomized Clinical Study

Bruksizm Varlığında ve Yokluğunda, Periodontal Sağlık ve Hastalıkta Vasküler Endotelyal Büyüme Faktörü, Hipoksi ile İndüklenebilir Faktör 1-Alfa ve Bakteriyel Plak Kompozisyonunun Değerlendirilmesi: Randomize Olmayan Klinik Çalışma

Nihat AKBULUT
Özkan KARATAŞ
Hatice BALCİ YUCE
Sibel AKBULUT
Ahmet ALTAN
Mehmet TAŞKAN
Pages
207–215
Publication Date
2021-05-29
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

However, one of the major oral pathogens, P. gingivalis, is af¬fected by the presence of bruxism, regardless of periodontitis.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: In this study, we aimed to evaluate the possible changes in hypoxia markers and bacterial compo-nents in patients with periodontitis and bruxism.
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Key Findings & Data:Methods: Four study groups were created: 20 healthy individuals without bruxism (Group 1), 20 periodontitis patients without bruxism (Group 2), 20 healthy individuals with bruxism (Group 3), and 20 periodontitis patients with bruxism (Group 4).
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Conclusion & Impact:However, one of the major oral pathogens, P. gingivalis, is af¬fected by the presence of bruxism, regardless of periodontitis.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:bruksizm · hiperkoklüzyon · hipoksi

Abstract

Aim: In this study, we aimed to evaluate the possible changes in hypoxia markers and bacterial compo-nents in patients with periodontitis and bruxism. Methods: Four study groups were created: 20 healthy individuals without bruxism (Group 1), 20 periodontitis patients without bruxism (Group 2), 20 healthy individuals with bruxism (Group 3), and 20 periodontitis patients with bruxism (Group 4). Plaque index, gingival index, and clinical attach-ment levels were recorded and gingival crevicular fluid (GCF) and dental plaque samples were taken. The GCF vascular endothelial growth factor (VEGF) and hypoxia-inducible factor 1-alpha (HIF-1α) levels were determined. Subgingival plaque samples were evaluated for 5 bacterial species (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, and Fu¬sobacterium nucleatum). Results: Compared to patients with periodontitis, plaque index, gingival index, and clinical attachment levels were significantly lower in healthy individuals. The GCF and serum levels of VEGF and HIF-1α were similar (p>0.05). The A. actinomycetemcomitans, T. denticola, and F. nucleatum counts did not differ significantly between the study groups (p>0.05). P. gingivalis counts were lowest in Group 1 and increased with both periodontitis and bruxism. T. forsythia counts were higher in Group 1 and Group 2 than in Group 3 and Group 4. The levels in Group 1 were lower than those in Group 2. Conclusion: Individuals with and without bruxism were found to be similar in terms of hypoxia in periodontal health and disease. However, one of the major oral pathogens, P. gingivalis, is af¬fected by the presence of bruxism, regardless of periodontitis.

Özet

Amaç: Bu çalışmada periodontitis ve bruksizm hastalarında hipoksi belirteçlerindeki ve bakteriyel bileşen¬lerdeki olası değişiklikleri değerlendirmek amaçlanmıştır. Yöntem: Dört çalışma grubu oluşturuldu: bruksizmi olmayan 20 sağlıklı birey (Grup 1), bruk-sizmi olmayan 20 periodontitis hastası (Grup 2), bruksizmi olan 20 sağlıklı birey (Grup 3) ve bruksizmi olan 20 periodontitis hastası (Grup 4). Plak indeksi, gingival indeks ve klinik ataçman seviyesi kaydedildi, dişeti oluğu sıvısı (DOS) ve dental plak örnekleri alındı. DOS’taki vasküler endotelyal büyüme faktörü (VEBF) ve hipoksi ile indüklenebilir faktör 1-alfa (HİF-1α) seviyeleri belirlendi. Subgingival plak örnekleri 5 bakteri türü (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola ve Fusobacterium nucleatum) için değerlendirildi. Bulgular: Plak indeksi, gingival indeks ve klinik ataçman seviyesi periodontitis hastalarına kıyasla sağlıklı bireylerde anlamlı biçimde daha düşüktü. DOS ve serum VEBF ve HİF-1α seviyeleri benzerdi (p>0,05). A. actinomycetemcomitans, T. denticola ve F. nucleatum sayıları çalışma grupları arasında anlamlı farklılık göstermedi (p>0,05). P. gingivalis sayıları Grup 1’de en düşük olup hem periodontitis hem de bruksizm ile artmaktaydı. T. forsythia sayıları Grup 1 ve Grup 2’de Grup 3 ve Grup 4’e göre daha yüksekti. Grup 1’deki seviyeler ise Grup 2’dekinden düşüktü. Sonuç: Bruksizmi olan ve olmayan bireyler, periodontal sağlık ve hastalıkta hipoksi bakımın¬dan benzer bulunmuştur. Bununla birlikte, başlıca oral patojenlerden biri olan P. gingivalis, periodontitisten bağımsız olarak bruksizm varlığından etkilenmektedir.

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Cite This Article

AKBULUT, N., KARATAŞ, Ö., YUCE, H. B., AKBULUT, S., ALTAN, A., TAŞKAN, M. (2021). Evaluation of Vascular Endothelial Growth Factor, Hypoxia-inducible Factor 1-Alpha, and Bacterial Plaque Composition in Periodontal Health and Disease, with or without Bruxism: A Non-Randomized Clinical Study. Anadolu Kliniği Tıp Bilimleri Dergisi, Cilt: 26 - Sayı: 2, 207–215. https://ajindex.com/articles/459276

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References (6)

  1. Caton JG, Armitage G, Berglundh T, Chapple IL, Jepsen S, Kornman KS, et al. A new classification scheme for periodontal and peri‐implant diseases and conditions— Introduction and key changes from the 1999 classifica¬tion. J Periodontol. 2018;89(Suppl. 1):S1–8. Murakami S, Mealey BL, Mariotti A, Chapple IL. Dental plaque–induced gingival conditions. J Clin Periodontol. 2018;45(Suppl. 20):S17–27. Tonetti MS, Greenwell H, Kornman KS. Staging and grading of periodontitis: framework and proposal of a new classification and case definition. J Periodontol. 2018;89(Suppl. 1):S159–72. Deshpande RG, Mhatre S. TMJ disorders and occlusal splint therapy—a review. Int J Dent Clin. 2010;2(2):22–9. Hallmon WW. Occlusal trauma: effect and impact on the periodontium. Ann Periodontol. 1999;4(1):102–8.
  2. Campos M, Corrêa M, Junior F, Casati M, Sallum E, Sal¬lum A. Cigarette smoke inhalation increases the alveo¬lar bone loss caused by primary occlusal trauma in a rat model. J Periodontal Res. 2014;49(2):179–85.
  3. Ispas A, Mihu C, Crăciun A, Constantiniuc M. Mor¬pho-histological assessment of the periodontal support structures under the action of excessive occlusal forces and under the influence of nicotine. Rom J Morphol Embryol. 2018;59(1):211. Grant DA, Grant DA, Flynn MJ, Slots J. Periodontal mi¬crobiota of mobile and non‐mobile teeth. J Periodontol. 1995;66(5):386–90.
  4. Cheng R, Liu W, Zhang R, Feng Y, Bhowmick NA, Hu T. Porphyromonas gingivalis-derived lipopolysaccha¬ride combines hypoxia to induce Caspase-1 activation in periodontitis. Front Cell Infect Microbiol. 2017;7:474. Takedachi M, Iyama M, Sawada K, Mori K, Yamamoto S, Morimoto C, et al. Hypoxia‐inducible factor‐1α inhib¬its interleukin‐6 and‐8 production in gingival epithelial cells during hypoxia. J Periodontal Res. 2017;52(1):127– 34. Ebersole JL, Novak MJ, Orraca L, Martinez‐Gonzalez J, Kirakodu S, Chen KC, et al. Hypoxia‐inducible tran¬scription factors, HIF1A and HIF2A, increase in aging mucosal tissues. Immunology. 2018;154(3):452–64.
  5. Gölz L, Memmert S, Rath-Deschner B, Jäger A, Appel T, Baumgarten G, et al. Hypoxia and P. gingivalis syn¬ergistically induce HIF-1 and NF-κB activation in PDL cells and periodontal diseases. Mediators Inflamm. 2015;2015:438085. Mendes RT, Nguyen D, Stephens D, Pamuk F, Fernandes D, Hasturk H, et al. Hypoxia‐induced endothelial cell re-sponses–possible roles during periodontal disease. Clin Exp Dent Res. 2018;4(6):241–8.
  6. Weng Y, Liu Y, Du H, Li L, Jing B, Zhang Q, et al. Glyco¬sylat