Journals / Avrupa Bilim ve Teknoloji Dergisi / 2020 / Cilt: 0 - Sayı: 20

Genetic Diversity of Emmer Wheats Using IPBS-RetrotranspozonMarkers

Bazı Gernik Buğdaylarında IPBS Retrotranspozon Markörleri Kullanılarak Genetik Çeşitlilik Analizi

Pages
640–646
Publication Date
2022-10-11
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Results obtained from this study showedthat iPBS molecular markers could be useful in determination of genetic relationships among wheat genotypes.

📌 3 Key Takeaways

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Objective & Hypothesis:The genetic diversity among totally 21 wheat genotypes (16 Triticum dicoccum L. called emmer wheat and 5 Triticum durum L. calleddurum wheat) were investigated using 7 iPBS primers. iPBSs used in the current study generated 134 polymorphic from out of 136bands for wheat genotypes.
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Key Findings & Data:Characterization of the iPBS primers were performerd by calculation meanpolymorphism informationcontent (PIC= 0.19), heterozygosity (H= 0.23), polymorphism ratio (P%= 97.92) and number of average polymorphic (19.14).
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Conclusion & Impact:Results obtained from this study showedthat iPBS molecular markers could be useful in determination of genetic relationships among wheat genotypes.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The genetic diversity among totally 21 wheat genotypes (16 Triticum dicoccum L. called emmer wheat and 5 Triticum durum L. calleddurum wheat) were investigated using 7 iPBS primers. iPBSs used in the current study generated 134 polymorphic from out of 136bands for wheat genotypes. Characterization of the iPBS primers were performerd by calculation meanpolymorphism informationcontent (PIC= 0.19), heterozygosity (H= 0.23), polymorphism ratio (P%= 97.92) and number of average polymorphic (19.14). Jaccard'sdiversity index varied between 0.0222 and 0.7843 with a mean 0.4677 for wheat genotypes. According to the sum of AMOVA, geneticdiversity in emmer and durum wheats was significantly high with 77% variation within the population. On the other hand, geneticvariation among populations was moderate with a value of 23%. Genetic diversity of populations were calculated by parametersincluding the mean number of alleles per locus (Na=1.269), effective allele number (Ne=1.31), Shannon information index (I=0.29),Nei’s genetic diversity level (h=0.19), and Nei’s unbiased genetic diversity level (uh=0.218). Results obtained from this study showedthat iPBS molecular markers could be useful in determination of genetic relationships among wheat genotypes.

Özet

Toplam 21 buğday genotipi (emmer buğdayı olarak adlandırılan 16 Triticum dicoccum L. ve durum buğdayı olarak adlandırılan 5Triticum durum L.) arasındaki genetik çeşitlilik 7 iPBS primeri kullanılarak araştırılmıştır. Mevcut çalışmada kullanılan iPBS'ler,buğday genotipleri için 136 banttan 134’ünü polimorfik olarak üretmiştir. Çalışmada kullanılan 7 iPBS primerinin karakterizasyonuortalama polimorfizm bilgi içeriği (PIC = 0.19), heterozigotluk (H = 0.23), polimorfizm oranı (P% = 97.92) ve polimorfizm (19.14)değerleri hesaplanarak belirlenmiştir. Jaccard'ın çeşitlilik değeri, buğday genotipleri için ortalama 0.4677 olarak hesaplanmış ve 0.0222ile 0.7843 arasında değiştiği saptanmıştır. AMOVA sonucuna göre gernik ve makarnalık buğdaylarındaki genetik çeşitlilik, popülasyoniçinde %77 varyasyona sahip olduğu tespit edilmiştir. Öte yandan, popülasyonlar arasındaki genetik varyasyon %23 değeriyle ortadüzeydeyde olduğu bulunmuştur. Popülasyonların genetik çeşitliliğini belirlemek için lokus başına ortalama alel sayısı (Na = 1.269),etkili alel sayısı (Ne = 1.31), Shannon bilgi indeksi (I = 0.29), Nei'nin genetik çeşitlilik seviyesi (h = 0.19) i parametreleri hesaplanmıştır.Ayrıca, Nei'nin tarafsız genetik çeşitlilik seviyesi (uh) 0.218 olarak saptanmıştır. Bu çalışmadan elde edilen sonuçlara göre iPBSmoleküler markörlerinin buğday genotipleri arasındaki genetik ilişkilerin belirlenmesinde faydalı olabileceğini göstermiştir.

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Cite This Article

DEMİREL, F. (2022). Genetic Diversity of Emmer Wheats Using IPBS-RetrotranspozonMarkers. Avrupa Bilim ve Teknoloji Dergisi, Cilt: 0 - Sayı: 20, 640–646. https://ajindex.com/articles/457841

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