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Journals / Atatürk Üniversitesi Ziraat Fakültesi Dergisi / 2004 / Cilt: 35 - Sayı: 3-4

Partially prification of glucose 6-phoshate dhydrogenase from bacillus sp. (BA-142) and investigation of some kinetic properties

Bacillus sp.(BA-142) bakterisinden glukoz 6-fosfat dehidrogenaz enziminin kısmen saflaştırılması

Mustafa ERAT
Ahmet ADIGÜZEL
Mehmet ÇİFTÇİ
Fikrettin ŞAHİN
Pages
151–158
Publication Date
2022-10-10
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Optimal pH, stable pH, optimal temperature, KM and V,^ values for NADP+ and glucose 6-phosphate (G 6 P) were also determined for the enzyme.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Glucose 6-phosphate dehydrogenase (G6PD) was purified from Bacillus sp.(BA-142) bacterium cells, and some characteristics of the enzyme were investigated.
📊
Key Findings & Data:After two consecutive steps, the enzyme, specific activity was 0.23 EU/mg protein, was purified with a yield of 79% and 82 fold purification.
💡
Conclusion & Impact:Optimal pH, stable pH, optimal temperature, KM and V,^ values for NADP+ and glucose 6-phosphate (G 6 P) were also determined for the enzyme.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Glucose 6-phosphate dehydrogenase (G6PD) was purified from Bacillus sp.(BA-142) bacterium cells, and some characteristics of the enzyme were investigated. The purification procedure was composed of two steps: homogenate preparation, and ammonium sulfate precipitation. After two consecutive steps, the enzyme, specific activity was 0.23 EU/mg protein, was purified with a yield of 79% and 82 fold purification. Optimal pH, stable pH, optimal temperature, KM and V,^ values for NADP+ and glucose 6-phosphate (G 6 P) were also determined for the enzyme.

Özet

Glukoz 6-fosfat dehidrogenaz enzimi (G6PD) Bacillus sp.(BA-I42) bakterisinden homojenatın hazırlanması ve amonyum sülfat çöktürmesi vasıtasıyla kısmi olarak saflaştırıldı ve bazı kinetik özellikleri araştırıldı. Saflaştırma prosedürü iki basamaktan oluştu; homojenatın hazırlanması ve amonyum sülfat çöktürmesi. Bu iki basamak sonucunda 0,23 EÜ/mg protein spesifik aktivitesine sahip enzim % 79 verimle 82 kat saflaştırıldı. Enzim için optimum pH, stabil pH, optimum sıcaklık değerleri bulundu. Ayrıca, NADP+ ve glukoz 6-fosfat (G6P) için KM ve VITMX değerleri tespit edildi.

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Cite This Article

ERAT, M., ADIGÜZEL, A., ÇİFTÇİ, M., ŞAHİN, F. (2022). Partially prification of glucose 6-phoshate dhydrogenase from bacillus sp. (BA-142) and investigation of some kinetic properties. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, Cilt: 35 - Sayı: 3-4, 151–158. https://ajindex.com/articles/456294

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