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Journals / Adıyaman Üniversitesi Fen Bilimleri Dergisi / 2020 / Cilt: 10 - Sayı: 1

LCMSMS Analysis of 8-OHdG and Measuring Metallothionein Level for Evaluation of Protective Role of Geraniol in Lead Acetate Administered Rats

Aysel ALKAN UÇKUN
Ertan YOLOĞLU
Miraç UÇKUN
Ahmet ÖZKAYA
Pages
100–111
Publication Date
2020-06-25
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to the results of this study, it can be suggested that geraniol protects cells against lead-caused damage by reducing ROS production.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, DNA damage and metallothionein levels were used as biomarkers to evaluate the protective potential of geraniol, a monoterpen against lead stress, in rats.
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Key Findings & Data:Hepatic 8-hydroxy-2-deoxyguanosine (8-OHdG) level used as a marker of oxidative DNA damage was measured by LCMSMS.
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Conclusion & Impact:According to the results of this study, it can be suggested that geraniol protects cells against lead-caused damage by reducing ROS production.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:8-hydroxy-2-deoxyguanosine · Metallothionein · Geraniol

Abstract

In this study, DNA damage and metallothionein levels were used as biomarkers to evaluate the protective potential of geraniol, a monoterpen against lead stress, in rats. Hepatic 8-hydroxy-2-deoxyguanosine (8-OHdG) level used as a marker of oxidative DNA damage was measured by LCMSMS. Experimental groups were formed in four ways: control, lead acetate, geraniol and lead acetate + geraniol. Seven animals were used in each group. Geraniol and lead acetate were administered to rats for 30 days. In geraniol administered rats, 8-OHdG and metallothionein levels decreased significantly compared to lead acetate administered rats. The highest DNA damage and metallothionein levels were observed in lead acetate administered rats. According to the results of this study, it can be suggested that geraniol protects cells against lead-caused damage by reducing ROS production. In addition, studies on the measurement of 8-OHdG by LCMSMS in the literature are limited. Therefore, it is thought that the presented study will contribute to the evaluation of the applicability of this method in the literature.

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Cite This Article

UÇKUN, A. A., YOLOĞLU, E., UÇKUN, M., ÖZKAYA, A. (2020). LCMSMS Analysis of 8-OHdG and Measuring Metallothionein Level for Evaluation of Protective Role of Geraniol in Lead Acetate Administered Rats. Adıyaman Üniversitesi Fen Bilimleri Dergisi, Cilt: 10 - Sayı: 1, 100–111. https://ajindex.com/articles/451039

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References (1)

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