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Journals / ARTEMİS / 2004 / Cilt: 5 - Sayı: 4

Komplet mol hidatidiformda bcl-2 ekspresyonu

bcl-2 Expression in complete hydatidiform mole

Müge HARMA
Mehmet İbrahim HARMA
İlyas ÖZARDALI
Journal
ARTEMİS
Pages
314–317
Publication Date
2022-10-11
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

Results for syncytiotrophoblasts were recorded semiquantitatively and analyzed by chi-square test.RESULTS: In tissue samples from patients with CHM, bcl-2 immunoreactivity occurred solely in the syncytiotrophoblasts and was significantly stronger than that found in normal placentas (11/15 moderate staining, 4/15 strong staining vs. 4/11 mild staining, 7/11 moderate staining; p

📌 3 Key Takeaways

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Objective & Hypothesis:OBJECTIVE: The objective of this study was to investigate the role of apoptosis in complete hydatidiform mole (CHM) by comparing apoptotic activity in CHM and normal placenta, using bcl-2 expression as the index of apoptotic activity.
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Key Findings & Data:MATERIALS-METHODS: Placental tissue samples were retrospectively analyzed from 15 patients with CHM and 11 healthy women undergoing first trimester termination of pregnancy.
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Conclusion & Impact:Results for syncytiotrophoblasts were recorded semiquantitatively and analyzed by chi-square test.RESULTS: In tissue samples from patients with CHM, bcl-2 immunoreactivity occurred solely in the syncytiotrophoblasts and was significantly stronger than that found in normal placentas (11/15 moderate staining, 4/15 strong staining vs. 4/11 mild staining, 7/11 moderate staining; p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

OBJECTIVE: The objective of this study was to investigate the role of apoptosis in complete hydatidiform mole (CHM) by comparing apoptotic activity in CHM and normal placenta, using bcl-2 expression as the index of apoptotic activity. MATERIALS-METHODS: Placental tissue samples were retrospectively analyzed from 15 patients with CHM and 11 healthy women undergoing first trimester termination of pregnancy. Diagnosis were confirmed histopathologically. After application of bcl-2, a classic avidin-biotin-peroxidase method and DAB chromogen were used for immunohistochemical analysis. Staining for bcl-2 was interpreted as positive (cytoplasmic staining) or negative (no staining reactivity). This immunopositivity was recorded separately for the different cellular components— cytotrophoblasts, syncytiotrophoblasts and intermediate trophoblasts. Results for syncytiotrophoblasts were recorded semiquantitatively and analyzed by chi-square test.RESULTS: In tissue samples from patients with CHM, bcl-2 immunoreactivity occurred solely in the syncytiotrophoblasts and was significantly stronger than that found in normal placentas (11/15 moderate staining, 4/15 strong staining vs. 4/11 mild staining, 7/11 moderate staining; p

Özet

Amaç: Bu çalışmanın amacı, apoptotik aktivite indeksi olarak bcl-2 ekspresyonunun kullanılmasıyla, komplet mol hidatidi-form (KMH) ve normal plasentalarda apoptotik aktiviteyi karşılaştırarak, KMH'de apoptozisin rolünü araştırmaktır. Materyal ve Metot: KMH'li 15 hasta ve ilk trimestr gebelik terminasyonu yapılan 11 sağlıklı kadının plasental doku örnekleri retrospektif olarak incelendi. Tanılar histopatolojik olarak doğrulandı, bcl-2 uygulanmasından sonra, immünohistokimyasal ölçüm için klasik avidin-biotin-peroksidaz metodu ve DAB kromogen kullanıldı, bcl-2 boyanması, pozitif (sitoplazmik boyanma) veya negatif (boyanma yok) olarak yorumlandı. Sitotrofoblastlar, sinsityotrofoblastlar ve intermediate trofoblastlardaki immünopozitiflik belirlendi. Sinsityotrofoblastlar için sonuçlar, semikantitatif olarak değerlendirildi ve ki-kare testi ile analiz edildi. Sonuçlar: KMH hastalarının doku örneklerindeki bcl-2 immünoreaktivitesi, yalnızca sinsityotrofoblastlarda normal plasentadakinden daha belirgin olarak bulundu (11/15 orta, 4/15 güçlü boyanmaya karşı, 4/11 hafif, 7/11 orta boyanma, p

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Cite This Article

HARMA, M., HARMA, M. İ., ÖZARDALI, İ. (2022). Komplet mol hidatidiformda bcl-2 ekspresyonu. ARTEMİS, Cilt: 5 - Sayı: 4, 314–317. https://ajindex.com/articles/449477

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