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Journals / Annals of Medical Research / 2021 / Cilt: 28 - Sayı: 11

Thymoquinone reduced RIPK1-dependent apoptosis caused by valproic acid in rat brain

Dilek Bayram
Sedat Bilgic
Deniz Tastemir Korkmaz
Sebile Azirak
Mehmet Kaya Ozer
Pages
2005–2011
Publication Date
2022-10-12
DOI
—
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Results: RIPK1 gene expression levels were significantly increased in the VPA group compared to the controls (p

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: Valproic acid (VPA) is a commonly used antiepileptic drug and known to have a neurotoxic effect, but its mechanism is not yet understood.
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Key Findings & Data:Materials and Methods: Male Sprague–Dawley albino rats were divided into three groups: control, VPA (500 mg/kg/day) and VPA + TQ (500 mg/kg/day + 50 mg/kg/day) with seven rats in.
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Conclusion & Impact:Results: RIPK1 gene expression levels were significantly increased in the VPA group compared to the controls (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: Valproic acid (VPA) is a commonly used antiepileptic drug and known to have a neurotoxic effect, but its mechanism is not yet understood. In the present study, we aimed to determine how the VPA causes cell death in the brain and to evaluate the protective effects of thymoquinone (TQ) on VPA-induced brain damage. Materials and Methods: Male Sprague–Dawley albino rats were divided into three groups: control, VPA (500 mg/kg/day) and VPA + TQ (500 mg/kg/day + 50 mg/kg/day) with seven rats in. At the end of the experiment, rats were sacrificed and brain samples were taken to measure the expression levels of Receptor-interacting serine/threonine-protein kinase-1 (RIPK1) and -3 (RIPK3) genes by quantitative real-time PCR (qRT-PCR), NADPH oxidase-4 (NOX4) and, caspase-3 (CAS-3) expression by immunohistochemistry and the structural changes in the brain tissue by histologically. Results: RIPK1 gene expression levels were significantly increased in the VPA group compared to the controls (p

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Cite This Article

Bayram, D., Bilgic, S., Korkmaz, D. T., Azirak, S., Ozer, M. K. (2022). Thymoquinone reduced RIPK1-dependent apoptosis caused by valproic acid in rat brain. Annals of Medical Research, Cilt: 28 - Sayı: 11, 2005–2011. https://ajindex.com/articles/445830

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