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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 1999 / Cilt: 46 - Sayı: 1

The investigation of rotavirus ifections in diarrheic and non-diarrheic sheep using PAGE and ELİSA .,

Klinik olarak sağlıklı veya diyare semptomlu koyunlarda rotavirus enfeksiyonunun enzyme lınked ımmunosorbent assay ( Elisa ) ve polyacrylamıde gel electrophoresis ( Page ) teknikleri ile araştırılması

T.Çiğdem OĞUZOĞLU
İbrahim BURGU
M.Tolga TAN
Feray ALKAN
Aykut ÖZKUL
Yılmaz AKÇA
Kadir YEŞİLBAĞ
Taner KARAOĞLU
Seval Bilge DAĞALP
Pages
143–147
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

At the end of this study, the presence of the Rotavirus infection were not detected in controlled lamb and sheep serologically and virologically.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this research, presence of Rotavirus antigen/nucleik as id was investigated using ELISA and PAGE technique in 40 diarrheic and 56 clinically normal lamb and 175 sheep in 5 intensive sheep flock.
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Key Findings & Data:Additionally, 298 serum samples collected from in 6 sheep flock were tested for antibodies by microneutralisation technique.
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Conclusion & Impact:At the end of this study, the presence of the Rotavirus infection were not detected in controlled lamb and sheep serologically and virologically.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this research, presence of Rotavirus antigen/nucleik as id was investigated using ELISA and PAGE technique in 40 diarrheic and 56 clinically normal lamb and 175 sheep in 5 intensive sheep flock. Additionally, 298 serum samples collected from in 6 sheep flock were tested for antibodies by microneutralisation technique. At the end of this study, the presence of the Rotavirus infection were not detected in controlled lamb and sheep serologically and virologically.

Özet

Bu çalışmada, 40 adedi ishalli, 56 adedi sağlıklı görünümlü 96 kuzu ile 175 koyuna ait 271 gaita örneği ELISA ve PAGE yöntemleri ile Rotavirus yönünden test edildi. Ayrıca, 6 farklı koyunculuk işletmesinde bulunan 298 adet koyun kan serumu örneğinde BRV Northern Ireland 75/447 susu kullanılarak uygulanan mikronötralizasyon testi ile Rotavirus antikorlarının varlığı araştırıldı. Elde edilen verilere göre, örneklenen koyunlarda rotavirus enfeksiyonunun varlığı serolojik veya virolojik olarak saptanamadı.

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Cite This Article

OĞUZOĞLU, T., BURGU, İ., TAN, M., ALKAN, F., ÖZKUL, A., AKÇA, Y., YEŞİLBAĞ, K., KARAOĞLU, T., DAĞALP, S. B. (2022). The investigation of rotavirus ifections in diarrheic and non-diarrheic sheep using PAGE and ELİSA .,. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 46 - Sayı: 1, 143–147. https://ajindex.com/articles/445458

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