Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2003 / Cilt: 50 - Sayı: 3

Pathological and immunohistocemical studies in experimental salmonella gallinarum infection (Fowl typhoid) in chickens

Tavuklarda deneysel salmonella gallinarum enfeksiyonunda (Tavuk tifosu) patolojik ve immunohistokimyasal çalışmalar

Pages
219–227
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

With immunoperoxidase staining strongly positive reaction were observed in the lungs and the intestines of one-day-old, whereas were observed in the bursa of Fabricius, the liver and spleen of the other groups.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, S. gallinarum 9 strain was inoculated orally in I,8xl09 doses to one-day-old, 2-week-old, one-month-old and 2-month-old White Leghorn chickens.
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Key Findings & Data:A bacterial suspension of 1 ml, 0,1 ml and 0,05 ml was used for each group.
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Conclusion & Impact:With immunoperoxidase staining strongly positive reaction were observed in the lungs and the intestines of one-day-old, whereas were observed in the bursa of Fabricius, the liver and spleen of the other groups.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, S. gallinarum 9 strain was inoculated orally in I,8xl09 doses to one-day-old, 2-week-old, one-month-old and 2-month-old White Leghorn chickens. A bacterial suspension of 1 ml, 0,1 ml and 0,05 ml was used for each group. After the inoculation, the animals were euthanasied on the twelveth day expect for the already dead ones. The animals were clinically, macroscopically, microscopically and immunohistochemically examined. The distribution of the morbidity and the mortality ratios with the lesions and the antigen were made according to the organs. Mortality rate figures for one-day-old and 2-week-old, one-month-old, 2-month-old groups were 95%, 86% and 43%, respectively. In one-day-old chicks lateral deviation and torticollis, diarrhea, depression, labored gasping were observed clinically. Lesions occurred showed variation depending on the age of affected birds and the dosage of agent. Macroscopically, it was observed gray white foci in the lungs and hemorrhages in the liver and the intestines of one-day-old chicks. It was also found hemorrhages in the thigh and pectoral muscles and involution of the bursa of Fabricius in chickens 2-week-old of age and one-month-old of age. Hemorrhages were usually present in the liver, lungs and the proventriculus of the 2-month-old chickens. The spleen was blackish in some groups while in some animals was light color. Gray white miliary foci were seen in the liver. Microscopically, pneumonia, lipidosis and passive congestion in the liver with haemorrhagic enteritis in the intestines and severe hemorrhages in the bursa of Fabricius were observed in chicks while pericarditis, diffuse parenchymatous hepatitis, necrotic enteritis were observed in adults. With immunoperoxidase staining strongly positive reaction were observed in the lungs and the intestines of one-day-old, whereas were observed in the bursa of Fabricius, the liver and spleen of the other groups.

Özet

Bu çalışmada Leghorn ırkı günlük, 2 haftalık, 1 aylık ve 2 aylık hayvanlara S. gallinarum 9 suşu I1SxIO9 CFU/M dozda, oral olarak inokule edildi. Her bir gruba 0,05 ml, 0,1 ml ve l ml bakteriyel süspansiyon uygulandı. Inokulasyondan sonra hayvanlar kendiliğinden ölenler dışında 12. günde ötanazi yapıldı. Bulgular klinik, makroskobik, mikroskobik ve immunohistokimyasal yönden değerlendirildi. Morbidite ve mortalite oranları ile lezyonların ve antijenin organlara göre dağılımı yapıldı. Mortalité günlük ve 2 haftalık civcivlerde %95, 1 aylık piliçlerde %86, 2 aylık piliçlerde ise %43 olarak bulundu. Klinik olarak, günlük civcivlerde lateral deviasyon ve tortikollis, ishal, gözlerde kapanma, solunum güçlüğü, tüylerde karışıklıkla seyreden depresyon ve ayakta duramama gözlendi. Oluşan lezyonlar hayvanların yaşı ve etkenin dozuna göre çeşitlilik gösterdi. Makroskobik olarak, günlük civcivlerde akciğerde boz beyaz odaklar, karaciğer ve bağırsaklarda konjesyon gözlendi, iki haftalık ve 1 aylık hayvanlarda pektoral ve bacak kaslarında kanama ve bursa Fabricius'da involusyon dikkati çekti, iki aylık piliçlerde bezli mide, karaciğer ve akciğerde konjesyon belirgindi. Dalak bazı gruplarda siyahımsı, bazı hayvanlarda ise oldukça açık renkteydi. Karaciğerde boz beyaz miller odaklara rastlandı, bursa Fabricius'da şiddetli kanama mevcuttu. Mikroskobik olarak, civcivlerde pnömoni, karaciğerde yağlanma ve pasif konjesyon ile bağırsaklarda hemorajik enteritis görülürken erişkinlerde perikarditis, diffuz paranşimatöz hepatitis ve nekrotik enteritise rastlandı. Immunoperoksidaz boyamada günlük civcivlerin özellikle akciğer ve bağırsaklarında diğer grupların ise bursa Fabricius, karaciğer ve dalaklarında güçlü pozitif reaksiyon gözlendi.

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Cite This Article

BEYAZ, L., KUTSAL, O. (2022). Pathological and immunohistocemical studies in experimental salmonella gallinarum infection (Fowl typhoid) in chickens. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 50 - Sayı: 3, 219–227. https://ajindex.com/articles/445301

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References (1)

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