AI Summary & Key Takeaways
AI InsightThe pharmakokinetic investigations showed that for both IV and intra-crop applications, especially in high dose (0.5-1 ppm) aflatoxin given groups (Groups IV-V), bioavailability of drug was lowered, its excretion become rapid, therefore, its terminal phase body half-life ($t_1$/2ß) was shortened.
📌 3 Key Takeaways
Abstract
In this study, total 170, daily, unvaccinated, male, Ross-PM3 strain, broiler chicks were used. Five main groups were designed; one of them was control and the others experimental groups. While the control group received normal food (without aflatoxin) during to 30 days, the experimental groups received feed with aflatoxin, orderly, 0.05 ppm (Group II), 0.1 ppm (Group III), 0.5 ppm (Group IV), and 1 ppm (Group V). On the 15th and 30th days of experiments, blood was taken and glucose, alanine aminotranferase (ALT), aspartate aminotransferase (AST), gamma-glytamil transferase (GGT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), cholesterol, triglyceride and total protein levels were evaluated. Beside this fact, body weight gain and their liver aflatoxin levels were determined. At the end of 30th day, 7 animals were taken from control and experimental groups and 10 mg/kg.bw enrofloxacin were administrated by IV and intra-crop and pharmacokinetic parameters calculated. It was conclusion that there were negative effects of aflatoxin in poultry, given doses above, for 30 days. The pharmakokinetic investigations showed that for both IV and intra-crop applications, especially in high dose (0.5-1 ppm) aflatoxin given groups (Groups IV-V), bioavailability of drug was lowered, its excretion become rapid, therefore, its terminal phase body half-life ($t_1$/2ß) was shortened.
Özet
Çalışmada, günlük, aşı uygulanmamış, 170 adet, erkek, Ross-PM3 ırkı, etçi civciv kullanılmıştır ve biri kontrol 4'ü deneme 5 grup oluşturulmuştur. Kontrol grubuna aflatoksin içermeyen, deneme gruplarına ise sırasıyla 0,05 ppm (Grup II), 0,1 ppm (Grup III), 0,5 ppm (Grup IV) ve l ppm (Grup V) total aflatoksin içeren yem otuz gün süreyle verilmiştir. Denemenin 15 ve 30. gününde alınan kanlarda glikoz, alanin aminotransferaz (ALT), aspartat aminotransferaz (AST), gamma-glutamil transferaz (GGT), alkalen fosfataz (ALP), laktat dehidrogenaz (LDH), kolesterol, trigliserid ve total protein düzeyleri değerlendirilmiştir. Ayrıca, canlı ağırlık kazancı ile 30. gün karaciğer aflatoksin düzeyleri tespit edilmiştir. Otuzuncu günün sonunda, kontrol ve deneme gruplarından 7'şer hayvan üzerinde damar içi ve kursak içi 10 mg/kg.ca dozunda enrofloksasin verilerek farmakokinetik çalışma yapılmıştır. Sonuç olarak, otuz gün süre ile belirtilen dozlarda verilen aflatoksinin kanatlılarda olumsuz etkilerinin olduğu, yapılan çok yönlü değerlendirmelerle anlaşılmıştır. Farmakokinetik çalışma sonucunda ise, hem kursak hem de damar içi uygulamalarda, özellikle de yüksek dozda (0,5-1 ppm) aflatoksin verilen gruplarda (Grup IV-V) ilacın, biyoyararlanımı düşmüş, atılımı hızlanmış dolayısıyla da vücutta kalış süresi kısalmıştır.