Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2005 / Cilt: 52 - Sayı: 3

Light and electron microscopical investigations on structure of the Turkey (Melagris gallopavo) kidney

Hindi (Meleagris gallopavo) böbreğinin yapısı üzerinde ışık ve elektron mikroskobik çalışmalar

Pages
149–155
Publication Date
2022-10-12
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Granule like dense bodies were found in the cytoplasm of the podocytes.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The aim of this study was to investigate the histological and histochemical structures of the nephrons and the juxtaglomerular apparatus of turkey kidney by light and electron microscopy.
📊
Key Findings & Data:In this study, tissue samples taken from healty and adult 5 male, 5 female turkeys which were used as materials.
💡
Conclusion & Impact:Granule like dense bodies were found in the cytoplasm of the podocytes.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to investigate the histological and histochemical structures of the nephrons and the juxtaglomerular apparatus of turkey kidney by light and electron microscopy. In this study, tissue samples taken from healty and adult 5 male, 5 female turkeys which were used as materials. It was determined that reptilian type nephrons were located at the periphery of the cortex, were simple and very numerous. The mammalian type nephrons laid within the deeper cortex, had a more complex structure and were not abundant. In addition histochemical methods showed that, the basement membrarie of the glomerular capillar was PAS (+), weakly Ab (+), the brush border and the basement membrane of the proximal tubule cells were PAS (+), the luminal surface of the distal tubule cells, except some of them, were weakly Ab (+), and the collecting tubule cells were PAS (+) and Ab (+). Juxtaglomerular granules generally were seen in the wall of the afferent arteriol and in some mesangial cells. At the end of Bowie's staining method, it was observed that, the juxtaglomerular granules dyed red or reddish purple. Granule like dense bodies were found in the cytoplasm of the podocytes.

Özet

Bu araştırmada, hindi böbreğinde nefronların ve jukstaglomerular aparatın histolojik ve histokimyasal yapılarının ışık ve elektron mikroskobik olarak incelenmesi amaçlandı Çalışmada materyal olarak sağlıklı ve erişkin 5 adet erkek, 5 adet dişi hindiden alınan doku örnekleri kullanıldı. Reptil tipi nefronların korteksin periferinde yer aldıkları, basit yapılı ye sayıca çok oldukları belirlendi. Memeli tipi nefronların derin kortekste yer aldıkları, daha kompleks bir yapıya sahip-oldukları ve sayıca az oldukları belirlendi. Ayrıca histokimyasal metodlar, glomerular kapilarların bazal membranlarının PAS (+), zayıf Ab (+), tubulus proksimalislerin fırçamsı kenarının ve bazal membranının PAS (+), tubulus distalis hücrelerinin luminal yüzeylerinin bazı hücreler hariç zayıf Ab (+), tubulus kolektivus hücrelerinin PAS (+), Ab (+) olduklarını gösterdi. Jukstaglomerular granüllerin aferent arteriyol ve bazı mesangiyal hücrelerde bulunduğu görüldü.Bowie boyama metodu sonucunda, granüllerin kırmızı veya kırmızı morumsu tonda boyandığı gözlendi. Podositlerin sitoplazmalarında granül benzeri, yoğun cisimlere rastlandı.

🔖

Cite This Article

AKAYDIN, Y., ÖZCAN, Z. (2022). Light and electron microscopical investigations on structure of the Turkey (Melagris gallopavo) kidney. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 52 - Sayı: 3, 149–155. https://ajindex.com/articles/445208

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (19)

  1. Aughey E, Frye FL (2001): Comparative Veterinary Histology with Clinical Correlates. Iowa State University Press, USA
  2. Bancroft JD, Cook HC (1984): Manual of Histological Techniques. Churchill Livingstone, Edinburgh
  3. Braun EJ, Dantzler WH. (1972): Function of mammalian-type and reptilian-type nephrons in kidney of desert quail. Am J Physiol, 222, 617-629
  4. Casotti G, Braun EJ (2000): Renal anatomy in sparrows from different environments. J. Morphol, 243, 283-291
  5. Culling CFA., Allison RT, Barr WD (1985): Cellular Pathology Technique. 4th Ed. Butterworth Co Ltd, London
  6. Denk H, Künzele H, Plenk H, Rüschoff J, Seline W (1989): Romeis Mikroscopische Technik
  7. Aufl. Urban und Schwarzenberg, München
  8. Goldstein DL, Skadhauge E (2000): Renal and extrarenal regulation of body fluid composition. Chapter 11. In: GC Whittow (Ed), Sturkie's Avian Physiology. 5th Ed., Academic Press, San Diego
  9. Harada K (1975): A rapid and simplified staining of juxtaglomerular granules with aqueous crystal violet. Microscopica acta, 77, 354-357
  10. Hodges RD (1974): The Histology of the Fowl. Academic Press, London
  11. Karnovsky MJ (1965): Formaldehyde-glutaraldehyde fixative of high osmolalitiy for use in electron microscopy. J Cell Biol, 27,137A-138A
  12. King AS (1993). Apparatus ürogenitalis. 329-346. In: JJ Baumel, AS King, JE Breazile, HE Evans, JC Van Denberge (Ed), Hand Book of Avian Anatomy: Nomina Anatomica Avium. 2nd Ed., Published by the Club, Cambridge
  13. Kon Y, Hashimoto Y, Kitagawa H, Kudo N (1984): Morphology and quantification ofjuxtaglomerular cells of the chicken kidney. Jpn J Vet Sci, 46, 189-196
  14. Malewitz TD, Calhoun ML (1958): The gross and microscopic anatomy of the digestive tract, spleen, kidney, lungs and heart of the turkey. Poult Sci, 37, 388-398
  15. Maxwell MH, Martindale L (1985): Electron microscopy of the juxtaglomerular apparatus in young fowl fed a salt deficient diet. Res Vet Sci, 39, 340-352
  16. Meseguer J, Ayala AG, Agullerio B (1987): Ultrastructure of the nephron of freshwater turtles, Pseudomys scripta elegans and Mauremys caspica. Cell Tissue Res, 248,381-391
  17. Miller RA (1967): Regional responses ofinterrenal tissue and of chromaffin tissue to hypophysectomy and stress in pigeons. Acta Endoc, 55, 108-118
  18. Morild I, Bohle A, Christensen JA (1985): Structure of the avian kidney. Anat Rec, 212, 33-40
  19. Morild I, Monwinckel R, Bohle A, Christensen JA (1985): The juxtaglomerular apparatus in the avian ki