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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2005 / Cilt: 52 - Sayı: 1

Determination of alpha naphtyl acetate esterase activtiy in peripheral blood leukocytes of camel (Cemelus dromedarius)

Develerin (Camelus dromedarius) perifer kan lökositlerinde alfa-naftil esatat esteraz aktivitesinin belirlenmesi

Mustafa SANDIKÇI
Şadiye KUM
Ülker EREN
Pages
13–16
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

However negative reactions were observed in erytrocytes and granulocytes.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study was carried out to determine the alpha naphthyl acetate esterase (ANAE) activity of peripheral blood leukocytes in camels.
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Key Findings & Data:Heparinized blood samples taken from' 13 adult camels were used.
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Conclusion & Impact:However negative reactions were observed in erytrocytes and granulocytes.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

This study was carried out to determine the alpha naphthyl acetate esterase (ANAE) activity of peripheral blood leukocytes in camels. Heparinized blood samples taken from' 13 adult camels were used. ANAE stainings were applied at different pH and durations for the determination of ANAE activity. The best reactions against ANAE were obtained for three hours stainings at pH 5.8. The proportion of ANAE positive T lymphocytes were 81.33 %. There was a diffuse granular pozitivity against ANAE staining in monocytes. However negative reactions were observed in erytrocytes and granulocytes.

Özet

Bu çalışma, develerde perifer kan lökositlerinin alfa naftil asetat esteraz (ANAE) aktivitesinin belirlenmesi amacıyla yapıldı. Çalışmada materyal olarak 13 adet erişkin deveden alınan heparinize kan örnekleri kullanıldı. ANAE aktivitesinin belirlenmesi için farklı pH ve sürelerde ANAE boyaması uygulandı. ANAE boyamasına karşı en iyi reaksiyon pH 5.8'de yapılan 3 saatlik boya-malardan elde edildi. Kanda ANAE pozitif T lenfosit oranı % 81.33 olarak tespit edildi. ANAE boyamasına karşı monositlerin diffuz granuler pozitivite gösterdikleri, eritrositlerin ve granulositlerin ise negatif reaksiyon verdiği dikkati çekti.

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Cite This Article

SANDIKÇI, M., KUM, Ş., EREN, Ü. (2022). Determination of alpha naphtyl acetate esterase activtiy in peripheral blood leukocytes of camel (Cemelus dromedarius). Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 52 - Sayı: 1, 13–16. https://ajindex.com/articles/445207

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References (3)

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