Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2006 / Cilt: 53 - Sayı: 3

The effects of uterine lavage and hormonal treatments on follicular activity and some fertility parameters in the onset of breeding season in mares with reproductive failure

Reprodüktif açıdan sorunlu kısraklarda aşım sezonu başlangıcında uterus yıkamaları ve hormon kullanımının siklus aktivitesi ve fertilite parametreleri üzerindeki etkisi

Pages
169–173
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

In conclusion, it was observed that uterine lavage and concurrent oxytocin administrations followed by various hormonal treatments were increased reproductive performance in stud farm mares with the history of infertility in previous breeding season.

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Objective & Hypothesis:The aim of this study was to investigate the effects of uterine lavage and concurrent oxytocin administrations fol- lowed by various hormonal treatments on follicular activity, induction of ovulation and pregnancy rates in mares with the history of infertility in previous breeding season.
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Key Findings & Data:Uterine lavages (8-10 litres of 0.9 % NaCl for at least 3 consecutive days until clean lavage fluid is observed) were done at the onset of oestrous before hormonal admini- strations and repeated after inseminations if necessary.
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Conclusion & Impact:In conclusion, it was observed that uterine lavage and concurrent oxytocin administrations followed by various hormonal treatments were increased reproductive performance in stud farm mares with the history of infertility in previous breeding season.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to investigate the effects of uterine lavage and concurrent oxytocin administrations fol- lowed by various hormonal treatments on follicular activity, induction of ovulation and pregnancy rates in mares with the history of infertility in previous breeding season. In this study, twelve different breed mares were used. Uterine lavages (8-10 litres of 0.9 % NaCl for at least 3 consecutive days until clean lavage fluid is observed) were done at the onset of oestrous before hormonal admini- strations and repeated after inseminations if necessary. Oxytocin was administered (20 IU i.v.) for 5 consecutive days. Mares were grouped as follows; Group I (n=4): intravaginal CIDR-B (1.9 g progesterone) in diestrous for 10 days combined with i.m. $PGF_2alpha$ on day 7 and on the day of CIDR-B removal in combination with i.m. estradiolbenzoate (5 mg) and subcutaneous desloreline acetate (DA; a GnRH analogue; 2.1 mg, Ovuplant® implant) as follicles reach the diameter of 30 mm. In group II (n=4); DA alone when follicles >30 mm are present. In group III (n=4); Control group, no hormonal treatments. Mean ovulation time following DA admini- strations was 41.16±8.15 h while ovulations occurred at 90.0+22.97 h after ultrasonographic detection of 35 mm diameter follicles in control group mares. Preovulatory follicles (>35 mm) were detected at 84.0±30.7 h following CIDR-B removal and ovulation of these follicles occurred at 50.7+15.4 h of reaching preovulatory diameter. Percentages of ovulations were 70%, 100% and 40% in groups I, II and III, respectively. Six mares (75%) became pregnant in Group I and II. However, due to embryonic losses, four mares carried their pregnancies to term. Only one (25%) mare became pregnant in the control group. In conclusion, it was observed that uterine lavage and concurrent oxytocin administrations followed by various hormonal treatments were increased reproductive performance in stud farm mares with the history of infertility in previous breeding season.

Özet

Bu çalışmanın amacı oksitosin destekli uterus yıkamalarını takiben yapılan hormon uygulamalarının önceki aşım sezonlarında birkaç kez çiftleştirilmelerine karşın gebe kalmayan kısraklarda follikül aktivitesi, ovulasyonun indüksiyonu ve gebelik sonuçları üzerindeki etkisini ortaya koymaktır. Çalışmada önceki aşım sezonlarında infertilite problemi görülen değişik ırklardan 12 kısrak kullanıldı. Intrauterin yıkama (8-10 litre %0.9 NaCl solüsyonu en az üç gün süreyle akıntı temiz görülene kadar) hormon uygulamalarından önce, östrus başlangıcında tüm kısraklara uygulandı ve çiftleşmelerden sonra sorun saptananlarda yıkamalar yenilendi. Oksitosin (20 IU, i.v.) beş gün uygulandı. Kısraklar 3 gruba ayrıldı; Grup I (n=4); İnteröstrus döneminde intravaginal CIDR-B (Controlled Internal Drug Release; 1.9g progesteron; 9 gün) uygulandı, uygulamanın 7. gününde $PGF_2alpha$ (Dinolytic® 5 mg, i.m.) ve CIDR-B çıkarılırken tekrar PGF2a ve östradiol benzoat (5 mg, i.m.) yapıldı. Folliküller 30 mm büyüklüğe ulaştığında deslorelin asetat (DA; GnRH-analoğu, 2.1 mg, Ovuplant® implant) subkutan uygulandı. Grup II (n=4); Bu grupta folliküller 30 mm büyüklüğe ulaştıktan sonra DA tek başına uygulandı. Grup III (n=4); kontrol gurubu olarak alındı ve herhangi bir uygulama yapılmadı. Deslorelin uygulandıktan sonra (Grup II) ovulasyon 41.16+8.15 saatte, kontrol grubunda ise folliküller 35 mm'ye ulaştıktan sonra 90.0±22.97 saat içinde gerçekleşti. CIDR-B çıkarıldıktan 84.0±30.9 saat sonra preovulatör folliküller (>35mm) gelişti ve sonrasında ovulasyonlar 50.7±15.4 saat içerisinde saptandı. Ovulasyon oranları Grup I, II ve III için sırasıyla %70, %100 ve %40 bulundu. Grup I ve Il'den 6 kısrak gebe kaldı (%75), embriyonik ölümler sonucunda 4 kısrak gebeliğini sürdürdü. Kontrol grubundan ise 1 kısrağın (%25) gebe kaldığı ve gebeliğini sürdürdüğü saptandı. Sonuç olarak; daha önceki sezonlarda gebe kalmamış kısraklarda oksitosin 4 eşliğinde yapılan uterus yıkamaları ve tedaviye yönelik yapılan hormon uygulamalarının fertilite yönünden sorunlu işletmelerde fertilite başarısını artıracağı gözlenmiştir.

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Cite This Article

ÇETİN, Y., KAÇAR, C., HANDLER, J., BECERİKLİSOY, H. B., AY, S. S., ZONTURLU, A. K., ASLAN, S. (2022). The effects of uterine lavage and hormonal treatments on follicular activity and some fertility parameters in the onset of breeding season in mares with reproductive failure. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 53 - Sayı: 3, 169–173. https://ajindex.com/articles/445183

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