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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2006 / Cilt: 53 - Sayı: 1

The effect of follicle stimulating hormone and human menopausal gonadotropin on in vitro maturation of bovine oocytes

Sığır oositlerinin in vitro maturasyonuna follikül uyarıcı hormon ve insan menopozal gonadotropinin etkisi

Hasan Ceyhun MACUN
Mustafa KAYMAZ
Pages
25–29
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Distribution of oocytes into different stages within the groups was significantly different (p

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to investigate the effects of FSH (Follicle Stimulating Hormone) and hMG (human Menopausal Gonadotropin) which are both easy to obtain and cheap when compared to other gonadotropins, on in vitro maturation of bovine oocytes.
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Key Findings & Data:After the in vitro maturation, Giemsa staining of oocytes in group I revealed that 14.36% of oocytes were at GV (Germinal Vesicle) stage, 6.38% of oocytes were at M I (Metaphase I) stage and 76.07% of oocytes were at M II stage while 3.19% of oocytes were degenerated.
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Conclusion & Impact:Distribution of oocytes into different stages within the groups was significantly different (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to investigate the effects of FSH (Follicle Stimulating Hormone) and hMG (human Menopausal Gonadotropin) which are both easy to obtain and cheap when compared to other gonadotropins, on in vitro maturation of bovine oocytes. According to hormone added to in vitro maturation media, three groups were formed; Group I (FSH), Group II (hMG) and Group III (Control). After the in vitro maturation, Giemsa staining of oocytes in group I revealed that 14.36% of oocytes were at GV (Germinal Vesicle) stage, 6.38% of oocytes were at M I (Metaphase I) stage and 76.07% of oocytes were at M II stage while 3.19% of oocytes were degenerated. The percentages of oocytes at stages GV, M I, M II and degenerated oocytes in groups II and III were 11.40%, 15.54%, 66.84%, 6.22% and 23.03%, 6.06%, 64.24%, 6.67% respectively. Distribution of oocytes into different stages within the groups was significantly different (p

Özet

Bu çalışmanın amacı; benzer gonadotropinlere göre daha ucuz olan ve kolay bulunabilen FSH (Follikül Uyarıcı Hormon) ve hMG (İnsan Menopozal Gonadotropini) hormonlarının sığır oositlerindeki in vitro maturasyon oranlarına etkisinin araştırılmasıdır. Oositlerin in vitro maturasyonu sırasında vasatlara eklenen hormonlara göre; FSH kullanılan Grup 1, hMG kullanılan Grup 2 ve kontrol grubu (Grup 3) olmak üzere 3 grup oluşturuldu. In vitro maturasyondan sonra, Grup 1 'de Giemsa ile boyaması yapılan oositlerin %14.36'sınm GV (Germinal Vezikül), %6.38'inin M I (Metafaz I), %76,07'sinin M II döneminde olduğu belirlenirken, %3.19'unun dejenere olduğu tespit edildi. Bu dururn-2. ve 3. Grupta sırasıyla %11.40; %15.54; %66.84; %6.22 ve %23.03; %6.06; %64.24; %6.67 olarak belirlendi. Gruplarda oluşan dağılımların birbirinden farklı olduğu tespit edildi (p

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Cite This Article

MACUN, H. C., KAYMAZ, M. (2022). The effect of follicle stimulating hormone and human menopausal gonadotropin on in vitro maturation of bovine oocytes. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 53 - Sayı: 1, 25–29. https://ajindex.com/articles/445161

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References (2)

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