Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2007 / Cilt: 54 - Sayı: 1

Diagnosis of infectious bronchitis in chickens by polymerase chain reaction and fliorescent antibody technique

Tavuklarda infeksiyöz bronşitisin polimeraz zincir reaksiyonu ve floresan antikor tekniği ile teşhisi

Pages
47–54
Publication Date
2022-10-12
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

RT-PCR was found to be superior to FAT in the identification of the disease and when performed after passage of suspected materials in embriyonated eggs both assays were observed to be important in determining the positive cases.

📌 3 Key Takeaways

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Objective & Hypothesis:In the study, a total of 461 materials, 42 of which werefrorn broilers, 18 were from broiler breeders and 11 were from layer flocks, were investigated.
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Key Findings & Data:In broiler flocks, clinically progressing respiratory disorders, coughing, srieezing, nasal discharge and in two (4.8%) flocks, at least one of the investigated birds was observed to have affected kidneys.
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Conclusion & Impact:RT-PCR was found to be superior to FAT in the identification of the disease and when performed after passage of suspected materials in embriyonated eggs both assays were observed to be important in determining the positive cases.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In the study, a total of 461 materials, 42 of which werefrorn broilers, 18 were from broiler breeders and 11 were from layer flocks, were investigated. In broiler flocks, clinically progressing respiratory disorders, coughing, srieezing, nasal discharge and in two (4.8%) flocks, at least one of the investigated birds was observed to have affected kidneys. Mortality rate was determined to change amongst flocks between 3-21%. In broiler breeder flocks, a drop by 2-11% in egg production, soft shelled eggs, drop in internal quality of eggs and affected kidneys in 2 (11.1%) flocks accompanying slight respiratory system problems were observed. Mortality rates in these flocks were found to be 3-8% in rearing flocks, 1-4% in laying flocks. In layer flocks, a decline between 6-14% in egg production and a decline in egg shell and internal quality accompanying respiratory distress were investigated. In rearing flocks while the mortalities were changing between 4-11% in layer flocks this ratio was found to change between 1-7%. After RT-PCR tests, IBV was detected in 16.7% of broiler flocks, 22.2% of broiler breeders and 27.3% of layers. IBV was detected from a total number of 14 out of 71 (19.7%) flocks, investigated. A positive reaction in 14.2% of materials obtained from broilers, 16.7% from broiler breeders and 18.2% from layers was observed by FAT. A total number of 11 (15.5%) out of 71 flocks were identified to have IB. According to these results, infectious bronchitis was found to be an important problem concerning chicken production in Turkey. RT-PCR was found to be superior to FAT in the identification of the disease and when performed after passage of suspected materials in embriyonated eggs both assays were observed to be important in determining the positive cases.

Özet

Çalışmada 42 broiler, 18 broiler damızlık ve 11 yumurtacı sürüden alman toplam 461 materyal değerlendirmeye alındı. Broiler kümeslerde, klinik olarak solunum bozukluğu, aksırık, tıksırık ve burun akıntısı olduğu ve 2 (% 4.8) kümeste, incelenen hayvanların en az birinde böbreklerin etkilendiği görüldü. Ölüm oranının, kümesler arasında % 3-21 arasında değiştiği belirlendi. Broiler damızlık sürülerde, hafif solunum sistemi bozukluğu ile birlikte yumurta veriminde % 2 ile % 11 arasında düşme, yumurta kabuklarında incelme, yumurtada pigment kaybı, yumurtanın iç kalitesinde bozulma ve 2 (% 11.1) kümeste böbrek lezyonlarına rastlandı. Ölüm oranı, büyütme dönemindeki kümeslerde % 3-8, verim dönemindeki kümeslerde % 1-4 olarak belirlendi. Yumurtacı sürülerde, solunum bozukluğunun yanısıra, % 6 ile % 14 arasında yumurta veriminde düşme, yumurta kabuk ve iç kalitesinde bozulma görüldü. Büyütme dönemindeki sürülerde ölüm oranı % 4-11 iken verim dönemindeki sürülerde bu oran % 1 ile % 7 arasında değişti. RT-PZR sonucunda, broilerlerde % 16.7, broiler damızlıklarda % 22.2 ve yumurtacı sürülerde % 27.3 oranında IBV pozitifliği belirlendi. Değerlendirmeye alınan toplam 71 kümesten 14 (% 19.7) kümeste IBV saptandı. FAT ile broiler kümeslerin %14.3'ünde, broiler damızlıkların % 16.7'sinde ve yumurtacı sürülerin % 18.2'sinde pozitif reaksiyon belirlendi. Toplamda ise FAT ile, 71 kümesin 11 (% 15.5)'inde IB pozitif olarak saptandı. Bu sonuçlara göre, Türkiye'de tavuklarda infeksiyöz bronşitisin önemli bir problem olduğu belirlenmiştir. Hastalığın teşhisinde polimeraz zincir reaksiyonunun floresan antikor tekniğine göre üstün ve pozitif vakaların ortaya konmasında embriyolu yumurta pasajının yararlı olduğu ortaya konmuştur.

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Cite This Article

SAREYYÜPOĞLU, B., AKAN, M., İZGÜR, M. (2022). Diagnosis of infectious bronchitis in chickens by polymerase chain reaction and fliorescent antibody technique. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 54 - Sayı: 1, 47–54. https://ajindex.com/articles/445143

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