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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2008 / Cilt: 55 - Sayı: 3

Sigara dumanına maruz bırkılan sıçanlarda lipid peroksidasyonu ve antioksidan enzimler

Lipid perioxidation and antioxidant enzymes in rats exposed to cigarette smoke

Pınar Alkım ULUTAS
Funda KIRAL
Ulvi Reha FIDANCI
Pages
145–148
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result, it was concluded that high plasma lipid peroxidation and low ascorbic acid concentrations may be involved in the pathogenicity of cigarette.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Oxidative metabolism in rats exposed to long term cigarette smoking was investigated in this study.
📊
Key Findings & Data:Fourty four rats (22 males and 22 females) were used in this study. 11 males and 11 female rats were assigned into cigarette smoke group and 11 male and 11 female rats were used as control group.
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Conclusion & Impact:As a result, it was concluded that high plasma lipid peroxidation and low ascorbic acid concentrations may be involved in the pathogenicity of cigarette.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Oxidative metabolism in rats exposed to long term cigarette smoking was investigated in this study. Fourty four rats (22 males and 22 females) were used in this study. 11 males and 11 female rats were assigned into cigarette smoke group and 11 male and 11 female rats were used as control group. The rats assigned into passive smoking were exposed to smoke in a unit for 120 minutes a day for five days a week during 16 weeks. At the end of this period, blood samples were collected from all rats. Plasma MDA and ascorbic acid analysis were performed immediately. Haemoglobin levels and GSH-px, SOD and CAT activities were determined in erythrocytes. The results were evaluated to student t-test. It was observed that plazma MDA levels and GSH-px, and CAT activities were high and plasma ascorbic acid levels were low compared with the control group in male and female rats. SOD activities showed no significant differences. As a result, it was concluded that high plasma lipid peroxidation and low ascorbic acid concentrations may be involved in the pathogenicity of cigarette.

Özet

Bu çalışmada uzun süre sigara dumanına maruz bırakılan sıçanlarda oksidatif metabolizma incelenmiştir. Çalışmada toplam 44 sağlıklı sıçan (22 erkek, 22 dişi) kullanılmıştır. Kontrol grubunda 11 erkek ve 11 dişi, sigara grubunda 11 erkek ve 11 dişi rat bulunmaktadır. Pasif dumana maruz bırakılan sıçanlara 16 hafta boyunca, haftada 5 gün, günde 120 dakika duman uygulanmıştır. Bu periyodun sonunda tüm hayvanlardan kan örnekleri toplanmıştır. Plazma MDA ve askorbik asit analizleri hemen yapılmıştır. Eritrositlerde hemoglobin düzeyleri ve GSH-px, SOD, CAT aktiviteleri belirlenmiştir. Sonuçlar student t-testi ile değerlendirilmiştir. Kontrol grubu ile karşılaştırıldığında erkek ve dişi sıçanların plazma MDA düzeyi ile GSH-px ve CAT aktivitelerinin yüksek, askorbik asit düzeylerinin düşük olduğu görülmüştür. SOD aktivitesi açısından ise her iki grup arasında istatistiksel farka rastlanmamıştır. Sonuç olarak, yüksek plazma lipid peroksidasyonu ve düşük askorbik asit düzeylerinin sigaranın patojenitesini arttırdığı düşünülmektedir.

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Cite This Article

ULUTAS, P. A., KIRAL, F., FIDANCI, U. R. (2022). Sigara dumanına maruz bırkılan sıçanlarda lipid peroksidasyonu ve antioksidan enzimler. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 55 - Sayı: 3, 145–148. https://ajindex.com/articles/445114

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