Türkiye'deki kuzularda abomazum lezyonlarından Clostridium sordellii izolasyonu

Objective & Hypothesis:In this study, the isolation of C. sordellii from abomasum lesions of lambs and the confirmation of the isolates by polymerase chain reaction (PCR) was attempted.

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Key Findings & Data:A total of 298 abomasal specimens (swabs) obtained from lambs of 4 to 8 months of age were investigated.

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Conclusion & Impact:In conclusion, the presence of C. sordellii in abomasum lesions was demonstrated in Turkey for the first time with this study.

🔬Design:Research Article

📍Scope:298 specimens

⚙️Variables:Domain Parameters

Abstract

In this study, the isolation of C. sordellii from abomasum lesions of lambs and the confirmation of the isolates by polymerase chain reaction (PCR) was attempted. A total of 298 abomasal specimens (swabs) obtained from lambs of 4 to 8 months of age were investigated. Sampled swabs were inoculated onto blood agar plates and incubated anaerobically. Following the incubation grown colonies were identified according to their morphological and biochemical characteristics. C. sordellii was isolated from 17 of 298 specimens (5.7%). These bacteria were also confirmed to harbour C. sordellii DNA by PCR. In conclusion, the presence of C. sordellii in abomasum lesions was demonstrated in Turkey for the first time with this study.

Özet

Bu çalışmada kuzuların abomazum lezyonlarından Clostridium sordellii izolasyonu ve izolatların polimeraz zincir reaksiyonu ile doğrulanması amaçlandı. Çalışma kapsamında 4-8 aylık yaştaki koyunlardan toplanan 298 abomazumdan alınan svab örnekleri incelendi. Sıvabların kanlı agarlara ekimi gerçekleştirilerek anaerobik ortamda inkube edildi. İnkubasyonu takiben şekillenen koloniler morfolojik ve biyokimyasal özelliklerine göre identifiye edildi. İncelen 298 materyalin 17 (% 5.7)’sinde C. sordellii izole edildi. İzolatlar PCR ile C. sordellii DNA’sı yönünden doğrulandı. Sonuç olarak, bu çalışma ile abomazum lezyonlarından C. sordellii izolasyonu Türkiye’de ilk kez gerçekleştirildi.

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Cite This Article

SAREYYÜPOĞLU, B., İLHAN, Z., ÖNCEL, T., AKAN, M., CANTEKİN, Z., TEL, Y. O. (2022). Türkiye'deki kuzularda abomazum lezyonlarından Clostridium sordellii izolasyonu. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 55 - Sayı: 2, 103–106. https://ajindex.com/articles/445108

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